Biologia plantarum 2010, 54:715-719 | DOI: 10.1007/s10535-010-0127-7

Analysis of the role of mitochondrial and endoplasmic reticulum localized small heat shock proteins in tomato

P. C. Nautiyal1,*, M. Shono2
1 Directorate of Groundnut Research, Junagadh, India
2 Okinawa Sub-tropical Research Station, Ishigaki, Japan

This communication examines the role of small heat shock proteins (sHsps) targeted to mitochondria (Mt) and endoplasmic reticulum (ER) in tomato plants (Lycopersicon esculentum Mill.) under heat stress. Genetic response of transgenic and wild type plants varied under optimum, moderately elevated and elevated temperature. In optimum temperature higher biomass was recorded in wild type than the transgenic lines, whereas in moderately elevated temperature biomass increased in Mt-sHsp line. Also, net photosynthetic rate (PN) increased in Mt-sHsp line in both the elevated temperatures, though higher in moderately elevated. Cell membrane stability (CMS) improved in all the lines after exposure to elevated temperatures, but always remained higher in transgenic lines. Transgenic lines expressed sHsps in different temperature regimes in both vegetative and reproductive parts, while wild type expressed such proteins only after 1 h of heat shock.

Keywords: cell membrane stability; elevated temperatures; Lycopersicon esculentum; plant growth; photosynthesis; thermotolerance
Subjects: Agrobacterium tumefaciens; chlorophyll a,b; electrolyte leakage; endoplasmatic reticulum; growth and growth analysis; heat shock proteins (HSP); leaf growth; Lycopersicon esculentum; mitochondria; photosynthetic rate; proteins; stomatal conductance; tomato; transgenic plants

Received: November 22, 2008; Accepted: October 15, 2009; Published: December 1, 2010Show citation

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Nautiyal, P.C., & Shono, M. (2010). Analysis of the role of mitochondrial and endoplasmic reticulum localized small heat shock proteins in tomato. Biologia plantarum, 54(4), 715-719. doi: 10.1007/s10535-010-0127-7.
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