Biologia plantarum 2010, 54:798-800 | DOI: 10.1007/s10535-010-0146-4

Chromosomal location of 45S rDNA and dfr gene in Citrus sinensis

X. Lu1,2, W. Zhou1, F. Gao1,*
1 School of Life Sciences, South China Normal University, Guangzhou, P.R. China
2 Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P.R. China

Dihydroflavonol 4-reductase (DFR) is a key enzyme in the anthocyanin biosynthesis. In this study, the localization of 45S rDNA and dfr gene (named as CsDFR-bo) of the blood orange (Citrus sinensis Osbeck cv. Ruby) on chromosomes was investigated by fluorescence in situ hybridization (FISH). A karyotype of C. sinensis was reconstructed based on the length of mitotic metaphase chromosomes. The 45S rDNAs were localized on chromosomes 2p and 7q. The detection ratios of 45S rDNA in the two chromosomes were 69.5 and 77.3 %, respectively. The CsDFR-bo was proved to be a single-copy gene and localized on chromosome 3p. The detection ratio of CsDFR-bo was 8.2 %.

Keywords: anthocyanin biosynthesis; dihydroflavonol 4-reductase gene; fluorescence in situ hybridization
Subjects: anthocyanin biosynthesis; anthocyanins; blood orange; chromosome characteristics; chromosome localization; Citrus sinensis; dihydroflavonol reductase; fluorescence in situ hybridization (FISH)

Received: April 30, 2009; Accepted: October 27, 2009; Published: December 1, 2010Show citation

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Lu, X., Zhou, W., & Gao, F. (2010). Chromosomal location of 45S rDNA and dfr gene in Citrus sinensis. Biologia plantarum54(4), 798-800. doi: 10.1007/s10535-010-0146-4.
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