Biologia plantarum, 1984 (vol. 26), issue 2

Article

Book reviews

Ingrid Tichá

Biologia plantarum 1984, 26:127 | DOI: 10.1007/BF02902277

Book reviews

Danuše Hodáňová, J. Šatava, Libuše Pavlová, J. Čatský, T. Gichner, Jana Pospíšilová

Biologia plantarum 1984, 26:154-158 | DOI: 10.1007/BF02902283

In memoriam of Professor Kurt Mothes

Milan KutáCek, Miloslav Sosna

Biologia plantarum 1984, 26:159 | DOI: 10.1007/BF02902284

Original Papers

Possibilities to reduce adverse effects of salinity by indole-3-acetic acid

A. M. Abdel-Rahman, A. H. Abdel-Hadi

Biologia plantarum 1984, 26:81 | DOI: 10.1007/BF02902270

Salinity caused a consistent reduction in the growth of cowpea plants and water content in their leaves. The total as well as the pigment fractions, except carotenoids, exhibited lower values than those of control plants at almost all salinity levels. With the rise of salinization, the total nitrogen and potassium contents in the leaves were decreased but the sodium content was increased and phosphorus content was not significantly affected as compared with the controls.
The application of IAA to salt-treated plants increased the water content in the leaves but it had no effect on the number of leaves and the stem length. The pigment contents in...

Induction of free radicals in seeds by high intensive flashes and the relevant phosphorus metabolism in the seedling

A. K. Srivastava, A. G. Chetverikov, S. A. Stanko, G. V. Novikova

Biologia plantarum 1984, 26:88-98 | DOI: 10.1007/BF02902271

Influence of high intensive flashes on the yield of free radicals in intact seeds and excised embryonic axis, endosperm, and seed coat, and its resulting effect on seedling growth, total biomass production and phosphorus metabolism in wheat (Triticum aeativum), vetch (Vicia sativa L.) and onion (Allium cepa L.) was studied. Free radicals (f.r.) were formed mainly in seed coat and not in the endosperm. Vetch seeds after irradiation had 20.76 X 1013 f.r. g-1 dry intact seed and 17.30 X 1013 f.r. g-1 dry seed coat. Excised seed coats exposed to irradiation also yielded 17.28 × 1013...

Detection and evaluation of serine proteinase by affinity chromatography on immobilized-aprotinin inRicinus communia

Tsuneo Watanabe, Noriaki Kondo, Kazutaka Kano

Biologia plantarum 1984, 26:99 | DOI: 10.1007/BF02902272

Neutral proteinase was found in the leaves ofRicinus communie as assayed with α-casein and H-D-Val-Leu-Lys-pNA as substrates. The enzyme is maximally active at pH around 7.4. A selective adsorbent for serine proteinase was prepared by attaching aprotinin to aminoalkyl-porous glass.
When partially purified leaf proteinase was passed through a column containing this adsorbent, the proteinase activity present was bound to the porous glass. The proteinase eluted at IM NaCl was inhibited by aprotinin, leupeptin, DFP, phenylmethylsulfonyl fluoride (PMSF) and serine proteinase inhibitor fromR, communis leaves, whereas pepstatin, EDTA,...

Metabolism of14C-abscisic acid during its IAA-promoted transport in etiolated segments of pea (Pisum sativum L.) epicotyls†

V. Borkovec, Hanno Lehmann, S. Prochazka

Biologia plantarum 1984, 26:108-112 | DOI: 10.1007/BF02902273

The metabolism of exogenously supplied abscisic acid (ABA) during translocation attracted under the influence of indolyl-3-acetic acid (IAA) was studied in etiolated segments of pea (Pisum sativum L.). After 8 and 24 h 90% and 60% of the ABA, respectively, were found in the segments in unchanged form. Phaseic acid, dihydrophaseic acid and the glucose ester of ABA were found as ABA metabolites. Results indicated that the growth processes initiated by the application of IAA were associated neither with an increased immobilization nor increased metabolization of this growth regulator.

Lectin fromCanavalia brasiliensis (MART.). isolation, characterization and behavior during germination

R. A. Moreira, B. S. Gavada

Biologia plantarum 1984, 26:113 | DOI: 10.1007/BF02902274

A lectin was isolated fromCanavalia brasiliensis Mart. seeds by combining solubility fractionation with affinity chromatography on Sephadex G-50. The lectin showed a carbohydrate specificity for D-mannose (D-glucose) binding and a requirement for Ca2+ and Mn2+. All the hemagglutinating activity was found in the cotyledons and the presence of the lectin was followed during the first 15 days of plant germination, through the activity against rabbit erythrocytes, the presence of the "lectin peak" in Sephadex G-50 affinity chromatography, presence of the "lectin bands" in SDS-polyacrylamide gel electrophoresis and the "lectin...

Specification of the effect of chelating complex of iron ions in androgenesisin vitro by means of cation-free minimal medium

J. Vagera, M. Jílek

Biologia plantarum 1984, 26:121 | DOI: 10.1007/BF02902276

The addition of Na2EDTA to a minimal medium (agar, sucrose) deprived of cations produces no morphoregulatory effect. This effect is induced only by the addition of iron. Traces of iron present in the agar are sufficient for the development of globular embryoid into a complete plant. The traces of iron in distilled water and sucrose (p. a.) are insufficient for the morphoregulation. The marked difference between the necessary limiting amount of chelate and necessary limiting amount of iron in non-modified media is explained by the presence of other cations, which saturate and thereby inactivate a greater part of Na2EDTA....

The effect of plasmolysis and deplasmolysis on the permeability of plant membranes

L. Adamec

Biologia plantarum 1984, 26:128 | DOI: 10.1007/BF02902278

The overall washing out of ions, especially86Rb+ (as the tracer for K+), from hypocotyl segments of pumpkin (Cucurbita pepo L.) into distilled water or a CaCl2 solution was studied, during plasmolysis with a saccharose solution and during deplasmolysis. Compartimental analysis was used to evaluate the86Rb+ washing out kinetics. During plasmolysis, the washing out of86Rb+ increases, due to two processes whose half-times are lower than those during washing out into the CaCl2 solution. During deplasmolysis, the permeability of plasmalemma and tonoplast...

Stabilization of the synthetic media for plant tissue and cell cultures

B. Vyskot, M. Bezděk

Biologia plantarum 1984, 26:132-143 | DOI: 10.1007/BF02902279

In standardMurashige-Skoog medium, particularly at pH higher than 5.0 and after heat sterilization, there is a tendency for turbidity or a sediment to appear, and for the acidity to increase by 0.2 to 0.5 degrees pH. The sediment is an amorphous precipitate of ferric phosphate and partly also of ferrous phosphate. In a stock iron solution prepared by chelation of ferrous sulphate with an equimolar quantity of the complexone Na2EDTA. up to 10% free FeII ions could be detected. By titration of a concentrated complexon solution it was found that in the presence of an excess of Na2EDTA (at the approximate molar ratio...

The effect of 9-(2,3-dihydroxypropyl) adenine (DHPA) on seedling roots ofVicia faba L. in comparison with adenine, adenosine and some cytokinins

K. Benes, A. Holý, O. Melichar

Biologia plantarum 1984, 26:144 | DOI: 10.1007/BF02902281

3-day-old seedlings ofVicia faba L. were put onto a diluted Knop's solution containing the tested substances within the intentional concentration range and left there for 7 days. In comparison with controls in plain nutrient solution, the DHPA treated plants revealed a suppression of shoot and root development. The main root growth was less sensitive than root branching. 8 h exposure was sufficient to gain a distinct effect. Microscopically the block of mitosis and/or cytokinesis, the formation of binucleate cells and local tissue damage were revealed. Under the given conditions, the cytokinins (BAP, (9R)BAP, iP, (9R)iP) proved to be much more...

Book Review

Ude, J., Koch, M.: Die Zelle. Atlas Der Ultrastruktur

Ingrid Tichá

Biologia plantarum 1984, 26:120 | DOI: 10.1007/BF02902275

Plimmer, J. R. (ed.): Pesticide Residues and Exposure.

T. Gichner

Biologia plantarum 1984, 26:143 | DOI: 10.1007/BF02902280

Brief Communications

Nitrate reductase, nitrite reductase activities and leaf diffusive resistance in wheat under water deficit

S. K. Sharma, O. P. Garg

Biologia plantarum 1984, 26:151 | DOI: 10.1007/BF02902282

The flag and second leaves of wheat showed physiological and biochemical differences in the normal and water stressed plants. Although the flag leaf had lower enzyme activities in the control plants, in stressed plants it could continue nitrate assimilation better than the second and other leaves. The flag leaf also exhibited a higher tendency to resume normal metabolic activity after the release of stress as is indicated by higher NRA, NiRa, lower rad and rab.