Biologia plantarum 2014, 58:411-418 | DOI: 10.1007/s10535-014-0409-6

Somatic embryogenesis and plantlet regeneration of Cassia angustifolia from immature cotyledon-derived callus

S. Parveen1, A. Shahzad1,*
1 Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, India

Plant regeneration through indirect somatic embryogenesis was attempted from the immature cotyledon-derived explant of Cassia angustifolia Vahl. - a valuable leguminous shrub. The highest frequency (90.5 %) of somatic embryos was obtained on a Murashige and Skoog (MS) medium augmented with 10.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 μM benzyladenine (BA) with the production of a maximum of 22.8 embryos per explant, of which 35.3 % germinated on the same medium after 6 weeks of culture. A half strength MS medium without plant growth regulators facilitated better conversion of embryos into complete plantlets compared to a full strength MS medium. Regenerated plantlets were successfully acclimatized in sterile Soilrite and transferred to field conditions with a 70 % survival rate. Histological studies performed at different stages of embryogenesis revealed the mode of differentiation of embryos from the callus. The content of chlorophylls (a + b) and carotenoids, and the net photosynthetic rate (PN) in the regenerated plantlets were tested during different periods of acclimatization.

Keywords: acclimatization; auxin; carotenoids; chlorophyll; cytokinin; histology; net photosynthetic rate; senna
Subjects: somatic embryogenesis; callus induction; auxin; cytokinin; ex vitro acclimatization; carotenoids; chlorophyll; histology; net photosynthetic rate; senna
Species: Cassia angustifolia

Received: July 4, 2013; Revised: October 8, 2013; Accepted: January 2, 2014; Published: September 1, 2014Show citation

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Parveen, S., & Shahzad, A. (2014). Somatic embryogenesis and plantlet regeneration of Cassia angustifolia from immature cotyledon-derived callus. Biologia plantarum, 58(3), 411-418. doi: 10.1007/s10535-014-0409-6.
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