Biologia plantarum 2014, 58:456-468 | DOI: 10.1007/s10535-014-0416-7

The expression of BrMDHAR gene in chloroplasts and mitochondria enhances tolerance to freezing stress in Arabidopsis thaliana

S. Y. Shin1,4, Y. S. Kim1, I. S. Kim1, Y. H. Kim3, H. M. Park3, H. S. Yoon1,2,*
1 Advanced-Bioresource Research Center, Kyungpook National University, Daegu, Republic of Korea
2 Department of Biology, Kyungpook National University, Daegu, Republic of Korea
3 National Institute of Crop Science, Rural Development Administration, Suwon, Republic of Korea
4 National of Horicultural & Herbal Science, Rural Development Administration, Suwon, Republic of Korea

In chloroplasts and mitochondria, antioxidant mechanisms include the ascorbate-glutathione cycle, and monodehydroascorbate reductase (MDHAR) is important for regeneration of ascorbate (AsA) from monodehydroascorbate (MDHA). To improve detoxification of reactive oxygen species (ROS), we established a construct of the MDHAR gene from Brassica rapa fused to the targeting signal peptides of Pisum sativum glutathione reductase (GR), which was controlled by a stress-inducible SWPA2 promoter, and introduced this expression system into Arabidopsis thaliana. Transgenic (TG) plants overexpressing BrMDHAR targeted to chloroplasts and mitochondria through signal peptides showed an elevated MDHAR activity and an increased ratio of AsA to dehydroascorbate (DHA) when compared to wild-type (WT) plants under a freezing stress. These led to increased photosynthetic parameters, redox homeostasis, and biomass in TG plants when compared to the WT plants. Our results suggest that the overexpression of the BrMDHAR gene targeted to chloroplasts and mitochondria conferred an enhanced tolerance against the freezing stress, and a stress adaptation of dual-targeted BrMDHAR was better than that of single BrMDHAR.

Keywords: ascorbate-glutathione cycle; ascorbate regeneration; Brassica rapa; chlorophyll content; dual targeting; Pisumsativum; redox homeostasis; ROS; transgenic plants
Subjects: monodehydroascorbate reductase; chloroplast; mitochondria; temperature - low; ascorbate-glutathione cycle; chlorophyll; redox homeostasis; transgenic plans; malondialdehyde; hydrogen peroxide; carotenoids
Species: Brassica rapa

Received: February 27, 2013; Revised: November 13, 2013; Accepted: January 10, 2014; Published: September 1, 2014Show citation

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Shin, S.Y., Kim, Y.S., Kim, I.S., Kim, Y.H., Park, H.M., & Yoon, H.S. (2014). The expression of BrMDHAR gene in chloroplasts and mitochondria enhances tolerance to freezing stress in Arabidopsis thaliana. Biologia plantarum, 58(3), 456-468. doi: 10.1007/s10535-014-0416-7.
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