Biologia plantarum - Latest articles

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Zinc-alleviating effects on iron-induced phytotoxicity in roots of Triticum aestivumOriginal Papers

T. Ma, X. H. Duan, Y. Y. Yang, J. Yao, T. P. Gao

Biologia plantarum 2017, 61:733-740 | DOI: 10.1007/s10535-017-0720-0

The mechanisms of growth inhibition and antioxidative response were investigated in wheat roots exposed to 300 μM iron together with different zinc concentrations (0, 50, and 250 μM). All Zn concentrations decreased Fe content but increased Zn content in the roots and leaves of Fe-treated seedlings. Compared with Fe stress alone, 50 or 250 μM Zn + Fe treatment stimulated root growth, and increased cell viability but decreased malondialdehyde content, which were correlated with the decreases of total and apoplastic hydrogen peroxide and superoxide anion radical (O₂ ^.-) content along with apoplastic hydroxyl radical content....

Application of X-ray absorption near edge spectroscopy to the study of the effect of sulphur on selenium uptake and assimilation in wheat seedlingsOriginal Papers

Q. Q. Huang, Q. Wang, Y. N. Wan, Y. Yu, R. F. Jiang, H. F. Li

Biologia plantarum 2017, 61:726-732 | DOI: 10.1007/s10535-016-0698-z

Selenium (Se) is an essential trace element for humans and animals. A hydroponic experiment was performed to study the effects of sulphur (S) on Se uptake, translocation, and assimilation in wheat (Triticum aestivum L.) seedlings. Sulphur starvation had a positive effect on selenate uptake and the form of Se supplied greatly influenced Se speciation in plants. Compared with the control plants, Se uptake by the S-starved plants was enhanced by 4.81-fold in the selenate treatment, and selenate was readily transported from roots to shoots. By contrast, S starvation had no significant effect on selenite uptake, and selenite taken up by roots was...

The cytotoxic targets of anatase or rutile + anatase nanoparticles depend on the plant speciesOriginal Papers

S. Silva, H. Oliveira, A. M. S. Silva, C. Santos

Biologia plantarum 2017, 61:717-725 | DOI: 10.1007/s10535-017-0733-8

The potential toxicity of nanoparticles (NPs) is under debate. Information about TiO₂ NPs phytotoxicity is still limited partly due to the different TiO₂ NP forms that may be found in the environment. The present work investigated the impact of different TiO₂ NPs forms (rutile and anatase) on germination, growth, cell cycle profile, ploidy level, and micronucleus formation in Lactuca sativa (lettuce) and Ocimum basilicum (basil). Seeds were exposed to anatase (ana) or rutile + anatase (rut+ana) at concentrations 5 - 150 mg dm^-3 for 5 d and after that different parameters were analyzed. Rut+ana...

Picea asperata pioneer and fibrous roots have different physiological mechanisms in response to soil freeze-thaw in springOriginal Papers

C. Yin, Q. Xiao, Y. Sun, Q. Liu, X. Pang

Biologia plantarum 2017, 61:709-716 | DOI: 10.1007/s10535-017-0728-5

About 70 % of the total land area in the world are affected by soil freeze and thaw (FT) cycles. Root is the first organ of plant to sense soil environment and it is unclear how it copes with the soil FT. Based on the different functions of firstorder pioneer and fibrous roots in woody plants, we hypothesize that pioneer and fibrous roots respond differently. The experiment was conducted in a growth chamber using Picea asperata seedlings. We designed the FT based on field observation data. The physiological responses in fibrous and pioneer roots were examined. Fibrous roots had higher root vitality and N content, whereas pioneer roots exhibited...

Non-thermal plasma modified growth and physiology in Triticum aestivum via generated signaling molecules and UV radiationOriginal Papers

A. Iranbakhsh, M. Ghoranneviss, Z. Oraghi Ardebili, N. Oraghi Ardebili, S. Hesami Tackallou, H. Nikmaram

Biologia plantarum 2017, 61:702-708 | DOI: 10.1007/s10535-016-0699-y

The current research was carried out to reveal the possible impacts of cold plasma on growth and physiology of wheat, as a new approach in plant science. Short and long-term impacts of different types of plasma (nitrogen and helium) with surface power density of 0.4 W cm^-2, exposure times (15, 30, 60, and 120 s), and repetitions (1, 2, and 4 times with 24 h intervals) were evaluated. Single-time applied helium or nitrogen derived plasma significantly promoted total root and shoot lengths, in contrast to four times application, and the root system was more sensitive than the shoot one. In addition, seedlings were more sensitive to nitrogen...

Overexpression of ERF96, a small ethylene response factor gene, enhances salt tolerance in ArabidopsisOriginal Papers

X. Wang, C. Hou, K. Zheng, Q. Li, S. Chen, S. Wang

Biologia plantarum 2017, 61:693-701 | DOI: 10.1007/s10535-017-0734-7

Salt stress is one of the abiotic stresses limiting the yield of crops worldwide. However, the molecular mechanisms underlying the regulation of plant response to salt stress are not completely elucidated. Ethylene response factors (ERFs) are a subfamily of the AP2 (APETALA2)/ERF transcription factor family that regulates multiple aspects of plant growth and development, and plant responses to biotic and abiotic stresses. ERF96 is one of the small ERFs that is involved in plant defense response and abscisic acid signaling in Arabidopsis. By using real time quantitative PCR, we found that the expression of ERF96 in the wild type Arabidopsis...

BrEXL6, a GDSL lipase gene of Brassica rapa, functions in pollen developmentOriginal Papers

R. Ji, H. Wang, X. Xin, S. Peng, Y. Hur, Z. Li, H. Feng

Biologia plantarum 2017, 61:685-692 | DOI: 10.1007/s10535-017-0735-6

Multiple allele-inherited male sterility has been widely used by breeders of Brassica rapa L. ssp. pekinensis, but the molecular mechanisms of male sterility are not yet clear. In this study, we isolated the full-length cDNA of a new gene (not included in the Brassica database). This gene, comprising 1 054 bp, encodes a 39.99 kDa protein with a Gly-Asp- Ser-Leu (GDSL)-lipase domain that is a member of the lipolytic protein GDSL family. The sequence of candidate gene is the most similar to extracellular lipase 6 (EXL6) of Arabidopsis and was therefore designated BrEXL6 and submitted to NCBI (accession...

Sulphur deficiency inhibits nitrogen assimilation and recycling in barley plantsOriginal Papers

C. G. Veliz, I. N. Roberts, M. V. Criado, C. Caputo

Biologia plantarum 2017, 61:675-684 | DOI: 10.1007/s10535-017-0722-y

Sulphur (S) is incorporated into diverse primary and secondary metabolites that play important roles in proper growth and development of plants. In cereals, a fraction of the nitrogen (N) accumulated in developing grains is guaranteed by amino acid remobilization from vegetative tissues, a contribution that becomes critical when soil nutrients are deficient. Glutamine synthetase (GS) and amino acid transporters (AAT) are key components involved in N assimilation and recycling. The aim of the present study was to evaluate the effect of S availability on the expressions of HvGS and several selected HvAAT genes in barley plants and on the...

RNA-seq analysis reveals a key role of brassinolide-regulated pathways in NaCl-stressed cottonOriginal Papers

H. M. Shu, S. Q. Guo, Y. Y. Gong, L. Jiang, J. W. Zhu, W. C. Ni

Biologia plantarum 2017, 61:667-674 | DOI: 10.1007/s10535-017-0736-5

Brassinolide (BL) alleviates salt injury in cotton seedlings; however, little is known about the molecular mechanisms of this response. In this study, digital gene expression analysis was performed to better understand the regulatory pathways of BL in NaCl-stressed cotton (Gossypium hirsutum L.). Compared with control plants (CK), a total of 1 162 and 7 659 differentially expressed genes (DEGs) were detected in the leaves and roots of NaCl-treated plants, respectively. Most of the DEGs in NaCl-treated plants, compared to CK, were regulated by BL. Moreover, expression patterns of DEGs in BL+NaCl treated plants were similar to those in CK plants;...

A protoporphyrinogen oxidase gene expression influences responses of transgenic rice to oxyfluorfenOriginal Papers

J. Park, L. H. Tran, S. Jung

Biologia plantarum 2017, 61:659-666 | DOI: 10.1007/s10535-017-0732-9

We compared the defense against photoinhibitions and oxidative stress in transgenic rice expressing a modified Myxococcus xanthus protoporphyrinogen oxidase (MxProtox) gene and in wild-type (WT) rice. Although the MxProtox transgenic lines had higher content of protoporphyrin IX (Proto IX) than the untreated controls, they did not exhibit a drastic accumulation of Proto IX as in the WT after 2 d of 50 μM oxyfluorfen (OF) treatment. In the transgenic lines S4 and S11, the transcriptions of OsProtox and modified MxProtox genes were almost sustained in response to OF, although transcription of OsProtox was...

Transcription factor NnDREB1 from lotus improved drought tolerance in transgenic Arabidopsis thalianaOriginal Papers

L. B. Cheng, J. J. Yang, L. Yin, L. C. Hui, H. M. Qian, S. -Y. Li, L. -J. Li

Biologia plantarum 2017, 61:651-658 | DOI: 10.1007/s10535-017-0718-7

Dehydration responsive element binding factor (DREB) is believed to be a stress-tolerance enhancer in plants. In the present study, a cold-binding factor (CBF)/DREB homologous gene NnDREB1 (XP_010242642.1) was isolated from lotus roots using rapid amplification of cDNA ends (RACE) and reverse transcription (RT)-PCR methods. Analysis of the deduced amino acid sequence and phylogeny classified NnDREB1 into the A-1 group of the DREB1 subfamily. Expression profiling using a quantitative PCR method revealed that NnRDEB1 was significantly induced by NaCl, mannitol, and polyethylene glycol, but not by low temperature and abscisic acid. To evaluate...

Na⁺/H⁺ and K⁺/H⁺ antiporters AtNHX1 and AtNHX3 from Arabidopsis improve salt and drought tolerance in transgenic poplarOriginal Papers

L. Yang, H. Liu, S. M. Fu, H. M. Ge, R. J. Tang, Y. Yang, H. H. Wang, H. X. Zhang

Biologia plantarum 2017, 61:641-650 | DOI: 10.1007/s10535-017-0724-9

The tonoplast and plasma membrane localized sodium (potassium)/proton antiporters have been shown to play an important role in plant resistance to salt stress. In this study, AtNHX1 and AtNHX3, two tonoplast Na⁺(K⁺)/H⁺ antiporter encoding genes from Arabidopsis thaliana, were expressed in poplar to investigate their biological functions in the resistance to abiotic stresses in woody plants. Transgenic poplar plants expressing either gene exhibited increased resistance to both salt and water-deficit stresses. Compared to the wild type (WT) plants, transgenic plants accumulated more sodium and potassium...

The identification of almond GIGANTEA gene and its expression under cold stress, variable photoperiod, and seasonal dormancyOriginal Papers

P. M. Barros, S. Cherian, M. Costa, H. Sapeta, N. J. M. Saibo, M. M. Oliveira

Biologia plantarum 2017, 61:631-640 | DOI: 10.1007/s10535-017-0711-1

Seasonal growth is characteristic for many tree species including almond. Varying conditions during the season are responsible for growth cessation, bud set, dormancy entry, cold hardening, and bud burst. Here, we report the characterization of an almond homologue of the Arabidopsis GIGANTEA (AtGI) gene (designated as PdGI, GenBank accession No. KJ502316). We propose a role for this gene in the transition to dormancy and cold acclimation. The complementary DNA (cDNA) sequence of PdGI was 4 322 bp long and contained an open reading frame of 3 512 bp. The deduced amino acid sequence of PdGI shared 76 % identity with AtGI. The expression...

Effect of salicylic acid on freezing injury in peach floral organs and the expressions of CBF genesOriginal Papers

B. B. Zhang, L. Guo, Z. Z. Song, M. L. Yu, R. J. Ma

Biologia plantarum 2017, 61:622-630 | DOI: 10.1007/s10535-017-0730-y

We used flowering branches at the budding stage of two peach cultivars Xiahui 6 and Xiacui with different cold resistance to explore the effect of exogenous salicylic acid (SA) on the freezing injury of peach floral organs and the molecular mechanism. Using water application as the negative control, the effects of spraying with SA at concentrations of 20 or 100 mg dm^-3 on stigma receptivity, frost damage characteristics of floral organs, and the expressions of C-repeat-binding factor (PpCBF) gene family members were investigated at 0 °C. No significant frost damage was observed on petals in all treatments. No frost damage was seen...

Two novel WRKY genes from Juglans regia, JrWRKY6 and JrWRKY53, are involved in abscisic acid-dependent stress responsesOriginal Papers

G. Y. Yang, W. H. Zhang, Y. D. Sun, T. T. Zhang, D. Hu, M. Z. Zhai

Biologia plantarum 2017, 61:611-621 | DOI: 10.1007/s10535-017-0723-x

Genes encoding plant WRKY transcription factors are important for stress response. In the current study, two WRKY transcription factor genes (JrWRKY6 and JrWRKY53) were identified from walnut (Juglans regia L.), and their function and involvement in stress responses were characterized. Under NaCl stress, JrWRKY6 and JrWRKY53 were upregulated in a short time (within 6 h of seedling exposure to salt) except in roots, in which the highest induction occurred at 24 and 48 h of salt exposure. The gene expression patterns under polyethylene glycol stress were similar to those under NaCl stress. Under heat stress,...

Cloning and characterization of four TpSnRK2s from dwarf Polish wheatOriginal Papers

Y. Jiang, Y. Wang, Z. Huang, H. Kang, L. Sha, X. Fan, H. Zhang, S. Gao, Y. Zhou, X. Yu

Biologia plantarum 2017, 61:601-610 | DOI: 10.1007/s10535-017-0719-6

Protein phosphorylation/dephosphorylation is a major signalling event induced by abiotic stresses in plants. Sucrose nonfermenting 1-related protein kinase 2 (SnRK2) plays important roles in response to osmotic stress. In the present study, four SnRK2s, TpSnRK2.1/3/7/8, were cloned and characterized from Triticum polonicum L. (dwarf Polish wheat, DPW, AABB). All of these were individually located on 2AL, 1AL, 2AL, and 5BL. Two spliced isoforms of TpSnRK2.8 (TpSnRK2.8a and TpSnRK2.8b) were observed. TpSnRK2.1 and TpSnRK2.3 were classified into the group II; TpSnRK2.7 was classified into the group I; and...

Lead induced oxidative stress and alteration in the activities of antioxidative enzymes in rice shootsBrief Communications

S. Thakur, L. Singh, A. W. Zularisam, M. Sakinah, M. F. M. Din

Biologia plantarum 2017, 61:595-598 | DOI: 10.1007/s10535-016-0680-9

Physiological responses of Oryza sativa L. to lead excess (10 and 50 μM) were studied in a hydroponic system after 48- and 96-h exposure. Accumulation of Pb in stressed rice shoots was concomitant with an increased metal concentration in the growth media and duration of exposure. The Pb stress resulted in an enhanced lipid peroxidation accompanied by altered activities of antioxidants. A substantial increase in α-tocopherol content of the Pb stressed rice shoots was observed suggesting its important role as an antioxidant. Among the antioxidant enzymes studied, activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX)...

Response of Arabidopsis thaliana root growth to phosphorus and its relation to media chemical compositionOriginal Papers

M. L. Strieder, K. G. Pinto, C. Bertoldi, A. de B. Schneider, C. A. Delatorre

Biologia plantarum 2017, 61:587-594 | DOI: 10.1007/s10535-017-0713-z

The interaction between phosphorus (P) and other media components alters root development and masks the plant response and thus limits the ability to correctly identify P-deficiency response (pdr) mutants. This study aims to assess changes in root development caused by different composition of growth media normally used in Arabidopsis research and to study their effects on pdr-mutant screening. Primary root growth of four genotypes was analyzed in media differing in P concentrations: half-strength Murashige and Skoog (1/2 MS) and Somerville and Ogren (SO). The effects of nitrogen source and Fe on root growth were investigated in...

Root characteristics of grafted peppers and their resistance to Fusarium solaniOriginal Papers

X. Duan, H. G. Bi, T. Li, G. X. Wu, Q. M. Li, X. Z. Ai

Biologia plantarum 2017, 61:579-586 | DOI: 10.1007/s10535-016-0677-4

Root rot caused by Fusarium solani, is one of the most severe diseases in pepper (Capsicum annuum L.). Grafting has been attempted as an effective means to control the disease, but little is known about the disease resistance mechanism in grafted pepper. Therefore, we investigated the changes of biomass, cell structure, and the secondary metabolism in roots of control (non-grafted pepper) and grafted peppers using cvs. Weishi and Buyeding as rootstocks and the cv. Xinfeng 2 as a scion. After a manual inoculation, less F. solani invaded grafted pepper roots and consequently less serious injury to the root cell ultra-structure compared...

Effects of melatonin on photosynthetic performance and antioxidants in melon during cold and recoveryOriginal Papers

Y. P. Zhang, S. J. Yang, Y. Y. Chen

Biologia plantarum 2017, 61:571-578 | DOI: 10.1007/s10535-017-0717-8

Melatonin (MT), a tryptophan derivative, plays an important role in the function and survival of organisms. To better understand the role of MT in cold tolerance, the melon (Cucumis melo L.) were sprayed with various concentrations of MT (0, 50, 100, 200 or 400 μM), exposed to cold stress (day/night temperature of 12/6 °C) for 7 d, and then returned to optimal conditions (28/18 °C) for 7-d recovery. The foliar application of MT (especially 200 μM) significantly alleviated cold-induced growth suppression, and MT-treated plants recovered more quickly than untreated plants. Further, MT-treated plants had higher chlorophyll content, photosynthetic...

Foliar-application of α-tocopherol enhanced salt tolerance of Carex leucochloraOriginal Papers

Y. R. Ye, W. L. Wang, C. S. Zheng, D. J. Fu, H. W. Liu, X. Shen

Biologia plantarum 2017, 61:565-570 | DOI: 10.1007/s10535-017-0709-8

Several different concentrations of α-tocopherol were applied to Carex leucochlora after plants had been treated with high salinity (0.8 % NaCl) in a greenhouse for one month. The results revealed that 0.8 mM α-tocopherol treatment showed the greatest alleviation of growth inhibition and cell membrane damage induced by salt stress. In comparison with NaCl alone, the 0.8 mM α-tocopherol application significantly decreased the content of hydrogen peroxide and the rate of superoxide radical generation, and increased the content of chlorophyll b, carotenoids, free proline, and soluble protein, but had no effect on the content...

Physiological and molecular responses to drought and salinity in soybeanOriginal Papers

H. R. Liu, G. W. Sun, L. J. Dong, L. Q. Yang, S. N. Yu, S. L. Zhang, J. F. Liu

Biologia plantarum 2017, 61:557-564 | DOI: 10.1007/s10535-017-0703-1

Drought and salinity are severe environmental stresses and limit soybean growth. In this study, a comparative analysis of physiological and molecular responses of two soybean (Glycine max L.) genotypes to these stresses was carried out. Plants of drought-tolerant genotype RD (cv. FD92) and sensitive genotype SD (cv. Z1303) were exposed to 15 % (m/v) PEG 6000, which simulated drought stress, or 150 mM NaCl. The RD plants maintained larger leaf area and higher net photosynthetic rate, chlorophyll content, stomatal conductance, and relative water content compared with the SD plants. Leaf proline content increased under both stresses more in RD...

Lanthanum improves the cadmium tolerance of Zea mays seedlings by the regulation of ascorbate and glutathione metabolismOriginal Papers

H. Dai, C. Shan, H. Zhao, G. Jia, D. Chen

Biologia plantarum 2017, 61:551-556 | DOI: 10.1007/s10535-016-0669-4

The effect of lanthanum on the metabolism of ascorbate (AsA) and glutathione (GSH) in the leaves of maize seedlings under cadmium stress was investigated. The findings showed that Cd remarkably increased electrolyte leakage (EL), the activities of ascorbate peroxidase, dehydroascorbate reductase, monodehydroascorbate reductase (MDHAR), glutathione reductase, L-galactono-1,4-lactone dehydrogenase, and γ-glutamylcysteine synthetase, and the content of reduced AsA, reduced GSH, total AsA, total GSH, malondialdehyde (MDA), and Cd, compared with control. However, Cd significantly decreased the dry biomass of roots and shoots. Treatment with...

Functional characterization of the antioxidant enzymes in rice plants exposed to salinity stressOriginal Papers

I. L. Vighi, L. C. Benitez, M. N. Amaral, G. P. Moraes, P. A. Auler, G. S. Rodrigues, S. Deuner, L. C. Maia, E. J. B. Braga

Biologia plantarum 2017, 61:540-550 | DOI: 10.1007/s10535-017-0727-6

The objective of this study was to relate the activation of enzymatic antioxidant system to the production of reactive oxygen species induced by salt stress. Rice (Oryza sativa L.) genotypes BRS Bojuru and BRS Pampa, tolerant and sensitive to salinity, respectively, were subjected to 150 mM NaCl for 0, 6, 24, 48, and 72 h. A significant increase of superoxide anion and H₂O₂ and a decrease in malondialdehyde (MDA) content were observed in the tolerant genotype, whereas in the sensitive genotype, there was no change in superoxide anion content, reduced H₂O₂ content, and increased MDA content. The superoxide...

Glucose-6-phosphate dehydrogenase plays critical role in artemisinin production of Artemisia annua under salt stressOriginal Papers

J. W. Wang, H. Tian, X. Yu, L. P. Zheng

Biologia plantarum 2017, 61:529-539 | DOI: 10.1007/s10535-016-0674-7

Artemisinin, a natural sesquiterpenoid isolated from Artemisia annua L., is regarded as the most efficient drug against malaria in the world. Artemsinin production in NaCl-treated A. annua seedlings and its relationships with the glucose-6-phosphate dehydrogenase (G6PDH) activity and generation of H₂O₂ and nitric oxide (NO) were investigated. Results revealed that artemisinin content in the seedlings was increased by 79.3 % over the control after 1-month treatment with 68 mM NaCl. The G6PDH activity was enhanced in the presence of NaCl together with stimulated generation of H₂O₂ and NO. Application...

Improving tobacco freezing tolerance by co-transfer of stress-inducible CbCBF and CbICE53 genesOriginal Papers

P. Lin, C. Shen, H. Chen, X. H. Yao, J. Lin

Biologia plantarum 2017, 61:520-528 | DOI: 10.1007/s10535-016-0687-2

Cold stress is one of the major limitations to crop productivity worldwide. We investigated the effects of multiple gene expression from cold tolerant Capsella bursa-pastoris in transgenic tobacco (Nicotiana tabaccum) plants. We combined CblCE53 and CbCBF into a reconstruct vector by isocaudomers. Plant overexpression of CbICE53 under the stress inducible CbCOR15b promoter and CbCBF under a constitutive promoter showed increased tolerance to both chilling and freezing temperatures in comparison to wild-type plants, according to the electrolyte leakage and relative water content. The expressions of endogenous...

Detection of DNA methylation pattern in thidiazuron-induced blueberry callus using methylation-sensitive amplification polymorphismOriginal Papers

A. Ghosh, A. U. Igamberdiev, S. C. Debnath

Biologia plantarum 2017, 61:511-519 | DOI: 10.1007/s10535-016-0678-3

During the normal developmental process, programmed gene expression is an essential phenomenon in all organisms. In eukaryotes, DNA methylation plays an important role in the regulation of gene expression. The extent of cytosine methylation polymorphism was evaluated in leaf tissues collected from the greenhouse grown plants and in in vitro-derived callus of three lowbush and one hybrid blueberry genotypes, using methylation-sensitive amplification polymorphism (MSAP) technique. Callus formation started from the leaf segments after 4 weeks of culture on a thidiazuron (TDZ) containing medium. Maximum callus formation (98 %) was observed in the...

Molecular characterization and subcellular localization of salt-inducible lipid transfer proteins in riceOriginal Papers

K.-C. Lin, T.-M. Wu, N. N. P. Chandrika, S.-J. Chou, C.-Y. Hong

Biologia plantarum 2017, 61:501-510 | DOI: 10.1007/s10535-016-0671-x

Rice (Oryza sativa L.) is a salt-sensitive species. Salt stress can cause injury to the plant cellular membrane. Plant lipid transfer proteins (LTPs) are abundant lipid binding proteins that are important in membrane vesicle biogenesis and trafficking, however, the biological importance of LTPs on salt-stress response in rice remains unclear. Therefore, salt-responsive rice LTPs were identified and characterized in this study. Microarray analysis showed seven genes positively regulated by salinity, including five Ltp genes (LtpII.3, LtpII.5, LtpII.6, LtpV.1, and LtpV.2) and two Ltp-like (LtpL;...

Constitutive expression of SlTrxF increases starch content in transgenic ArabidopsisOriginal Papers

F. B. Wang, W. L. Kong, Y. R. Fu, X. C. Sun, X. H. Chen, Q. Zhou

Biologia plantarum 2017, 61:494-500 | DOI: 10.1007/s10535-016-0675-6

The plastidic thioredoxin F-type (TrxF) protein plays an important role in plant saccharide metabolism. In this study, a gene encoding the TrxF protein, named SlTrxF, was isolated from tomato. The coding region of SlTrxF was cloned into a binary vector under the control of 35S promoter and then transformed into Arabidopsis thaliana. The transgenic Arabidopsis plants exhibited increased starch accumulation compared to the wild-type (WT). Real-time quantitative PCR analysis showed that constitutive expression of SlTrxF up-regulated the expression of ADP-glucose pyrophosphorylase (AGPase) small subunit (AtAGPase-S1...

Identification of alternatively spliced MsRan transcripts involved in low temperature response in Musa spp.Original Papers

Y. L. Zhang, Z. Z. Fang, Z. X. Lai

Biologia plantarum 2017, 61:483-493 | DOI: 10.1007/s10535-016-0682-7

Ran is involved in response to external stimuli. In this study, six MsRan gene cDNA sequences were isolated from wild banana (Musa spp. AB group) from Sanming City, China. Sequence analysis reveals that MsRan3A, MsRan3A-1a, and MsRan3C contained Ran protein domains including a GTP hydrolysis domain, a RanGAP-binding domain, and an acidic tail, whereas two G boxes (G4 and G5) were absent in MsRan3A-6a. The physicochemical property of MsRan3A, MsRan3A-1a, MsRan3A-6a, and MsRan3C appeared to differ significantly. Real time quantitative PCR (qPCR) analysis indicates that MsRan3A-1, MsRan3A-5, MsRan3A-6, MsRan3A-6a,...