Book Review

Ingrid Tichá

Biologia plantarum 1990, 32:428 | DOI: 10.1007/BF02890888

Book Review

Ingrid TichÁ

Biologia plantarum 1990, 32:444 | DOI: 10.1007/BF02890891

Book Review

Jarmila Solárová, Jana Pospisilova, M. Ondřej, M. Juřiček, T. Gichner, M. Ondřej, Th. Gaspar, V. Novák, Jarmila Solárová

Biologia plantarum 1990, 32:474-480 | DOI: 10.1007/BF02890895

Potato transformation by T-DNA Cytokinin synthesis gene

M. Ondrej, Ivana MachÁĉková, J. ĈatskÝ, J. Eder, M. Hrouda, Jana Pospisilová, Helena Synkova

Biologia plantarum 1990, 32:401 | DOI: 10.1007/BF02890884

Potato plants were transformed by the A.tumefaciens vector which integrates into the plant genome two foreign genes only: pTi C58 T-DNA gene 4 (ipt) responsible for elevated synthesis of cytokinins and kanamycin resistance gene. Three teratoma clones studied showed approximately 3 times, 6 times and 9 times increased levels of zeatin riboside and zeatin in comparison with untransformed controls and slight increase of the IAA level. Shoots formed roots in agar cultures sporadically, if the increase of zeatin riboside and zeatin levels was not higher than 6 times the control level. The chlorophyll content and net photosynthetic rate decreased...

Stimulation of the regeneration capacity of tree shoot segment explantsin vitro

Blanka Civínová, Z. Sladký

Biologia plantarum 1990, 32:407 | DOI: 10.1007/BF02890885

Regeneration abilities of buds on shoot segment explants isolated from adult trees of oak (Quercus robur), aspen (Populus tremula), black locust (Robinia pseudacacia), Japan pagoda tree (Sophora japonica), and English walnut (Ailanthus glandulosa) were studied during the growing season. Optimum BAP concentrations for the regeneration of oak bud meristems were dependent on the date of sampling. Axillary shoots could be induced from winter and summer buds of oak and aspen on Dustan and Short media supplemented with activated charcoal and BAP at concentrations from 0.5 to 2 mg 1^-1. More intensive rooting of...

Somatic embryogenesis and cytoplasmic sterility inBeta vulgaris L. var.saccharifera

Marie KubalÁkovÁ

Biologia plantarum 1990, 32:414-419 | DOI: 10.1007/BF02890886

Embryogenic callus which has maintained its embryogenic ability on media without growth regulators for three years has been induced at the base of shoots of a genotype with CMS propagated for a long timein vitro by transferring the shoots onto media richer in inorganic and organic components. The effect of two basal media (MS and PG₀) on the intensity and completeness of the proliferation of somatic embryos was examined with different combinations of growth regulators. Pollen fertility was evaluated in 87 plants regenerated from somatic embryos. Cytoplasmic male sterility was conserved in all of them.

Photophysiology of turion germination in Spirodela polyrhiza (L.) Schleiden. The cause of germination inhibition by overcrowding

K. -J. Appenroth, Waltraud Hertel, H. Augsten

Biologia plantarum 1990, 32:420 | DOI: 10.1007/BF02890887

Red-light-induced (via phytochrome) germination decreased with increasing numbers of turions per germination flask (overcrowding). Three hypotheses concerning the mechanism of this germination inhibition were tested, related to abscisic acid, ethylene, and oxygen deficiency: (i) Although abscisic acid is a powerful inhibitor of turion germination it had to be excluded as a cause, because abscisic acid was not secreted from turions into the nutrient solution, (ii) Ethylene (ethrel) strongly inhibited growth of newly formed sprouts, but germination response itself was not inhibited, (iii) Germination inhibition did not appear if short light pulses...

Study of sunflower (Helianthus annuus L.) seed globulin: Accumulation and intracellular localization

E. Ferjani, G. Ledoigt

Biologia plantarum 1990, 32:429 | DOI: 10.1007/BF02890889

Protein synthesis and accumulation in growing sunflower (Helianthus annuus L.cv.Airelle) seeds are studied. The salt soluble fraction, globulin, is the main soluble protein component. The earlier stages of seed development (10 days after flowering) are characterized by high M_r polypeptides (74, 58 and 44 kDa). Later stages mainly show nature globulin polypeptides. Thus, protein synthesis in seed occurs at a specific period of seed development which follows a period of fast cell divisions (0-14 days after flowering). Protein bodies are isolated and their protein composition analyzed. Globulin subunits are the main polypeptides of protein...

Proteolytic activities in the developing seeds of ×Haynaldoticum sardoum

A. Capocchi, L. Galleschi, P. Meletti

Biologia plantarum 1990, 32:436-444 | DOI: 10.1007/BF02890890

Aminopeptidase, carboxypeptidase and proteinase activities were measured in endosperms from unripe and ripe seeds of ×Haynaldoticum sardoum. Aminopeptidase and proteinase activities were high during the early maturation stages and then decreased. In contrast, carboxypeptidase activity increased with maturation.
Localization studies demonstrated that aminopeptidase and carboxypeptidase activities were present in the three tissues examined (pericarp, green layer plus aleurone, and starchy endosperm). Proteinase activity against gliadin was located in the pericarp and in the green layer plus aleurone, but was absent in the starchy endosperm.
The...

ABam HI family of tobacco highly repeated DNA:

Blažena Koukalová, J. Reich, M. Bezděk

Biologia plantarum 1990, 32:445-449 | DOI: 10.1007/BF02890892

ABamHI family of highly repeated DNA sequences of theNicotiana tabacum nuclear genome, denoted as a HRS60-family, was recently isolated. It comprises about 2% of the tobacco nuclear genome. Monomeric units are 182-184 bp long. Members of the HRS60-family isolated till now are closely related. DNA-DNA hybridization experiments with DNA of the two tobacco progenitors,N. tomentosiformis andN. sylvestris, revealed that the HRS60-family was present in many copies inN. sylvestris, the amount being about 1.7 times that inN. tabacum. InN. tomentosiformis as well as in some other species of the genusNicotiana,...

Phyllochron and winter barley leaf growth rate

Jana Tesařová, L. Nátr

Biologia plantarum 1990, 32:450 | DOI: 10.1007/BF02890893

Growth rate of leaves of winter barley (Hordeum vulgare L. cv. Efra) plant in thermal time correlated with the final length of the corresponding leaf (r = 0.66). The sum of effective temperatures corresponding to the time of growth of a particular leaf linearly increased with leaf insertion (r = 0.82). Phyllochron value in plants grown in the field with either natural or controlled photoperiod was dependent on phyllochron order, especially up to the double ridge stage (r = 0.89 and r = 0.74, respectively). The mean value of phyllochrons in the field variants showed a very good agreement with the value calculated on the basis of the change in...

An immunochemical study of antigen expression in potato spindle tuber viroid (PSTV) - infected tomato leaves and calluses

J. Matoušek, P. Dědič, M. J. Beneš, P. Kopáček, Věra Turková, Ludmila Trněná

Biologia plantarum 1990, 32:460-473 | DOI: 10.1007/BF02890894

A polyspecific antiserum against protein extracted from PSTV-infected tomato leaves was prepared and the IgGs were separated by affinity chromatography on a beaded cellulose adsorbent with an immobilized "healthy" antigen. The antibody not adsorbed entered into a preferential reaction with the antigen from PSTV-infected leaves as estimated by an enzyme-linked immunosorbent assay. The immunochemical reactions did not significantly exceed the control background, if antigens from tomato leaves infected with potato viruses X, Y and M were analyzed. By immunoblot technique we revealed, however, that several antigens not detected in healthy leaves appeared...