Biologia plantarum 2013, 57:684-690 | DOI: 10.1007/s10535-013-0347-8

Cucumber BAX inhibitor-1, a conserved cell death suppressor and a negative programmed cell death regulator under cold stress

X. H. Chen1, H. Yu2, H. J. Deng1, J. X. Chen1, H. B. Mi1, L. C. Mao1,*
1 College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, P.R. China
2 Institute of Insect Sciences, Zhejiang University, Hangzhou, P.R. China

Programmed cell death (PCD) is a genetically controlled and conserved process in eukaryotes during development as well as in response to pathogens and other stresses. BAX inhibitor-1 (BI-1) has been implicated as an anti-PCD factor which is highly conserved in plants. Sequence of putative cucumber BI-1 protein exhibited 77.7 % identity and 91.2 % positive value with the homologue Blast BI-1 protein of Arabidopsis thaliana (AtBI-1). This highly homologous protein to the AtBI-1 protein was named CsBI-1. This protein contains an open reading frame (ORF) of 250 amino acids with a BAX inhibitor domain and five transmembrane regions conserved among members of the BI-1 family. Primers designed by the cDNA of CsBI-1gene were used for further sequencing. Cell death in cold-stored cucumber developed concomitantly with increased expression of the CsBI-1 gene and reached maximum at day 6. However, cell death accelerated significantly after 9 d when sharp decrease of the CsBI-1 expression occurred. After warming to 20 °C, expression of the CsBI-1 gene was the highest at day 3, decreased afterwards, and the lowest expression was detected at day 9 when PCD obviously appeared. The overall results indicate that CsBI-1 is cucumber homologue of Arabidopsis thaliana AtBI-1 gene. CsBI-1 is a conserved cell death suppressor induced by cold stress and a negative regulator of PCD.

Keywords: Arabidopsis thaliana; Cucumis sativus; RT-PCR
Subjects: BAX inhibitor; cell death; temperature - low; phylogenetic tree; amino acid sequence; cucumber
Species: Cucumis sativus

Received: January 11, 2013; Accepted: March 18, 2013; Published: December 1, 2013Show citation

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Chen, X.H., Yu, H., Deng, H.J., Chen, J.X., Mi, H.B., & Mao, L.C. (2013). Cucumber BAX inhibitor-1, a conserved cell death suppressor and a negative programmed cell death regulator under cold stress. Biologia plantarum, 57(4), 684-690. doi: 10.1007/s10535-013-0347-8.
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