Biologia plantarum, 1968 (vol. 10), issue 6
Article
Water balance of plants during root application of high concentrations of growth substances
V. Kozinka
Biologia plantarum 1968, 10:398 | DOI: 10.1007/BF02920983
Using hydroponic cultures, the effect of high concentrations (10-3 m) of 2-methyl-4-chlorophenoxyacetic acid (MCPA) in the root medium on the water balance of 8-9 week old plants ofPisum sativum L. and of 9-10 week-old plants ofSinapis alba L. was studied. The water balance was determined in the light and in the dark gravimetrically by measuring the intensity of water uptake and transpiration in plants cultivated by the method of root bridges according to Werner. MCPA present in the root medium in illuminated plants decreased rapidly the intensity of both the uptake and the loss components of the water balance. In permanent...
Contribution to the estimation of nucleic acids in wheat roots
Svatava Fialová
Biologia plantarum 1968, 10:409 | DOI: 10.1007/BF02920984
Several methods of NA isolation and estimation were examined in order to determine RNA in the whole root systems of young wheat plants. It was found out that during the hydrolysis of roots with perchloric acid according toOgur andRosen (1950),Spirin (1958) andHeitefuss (1965) a considerable amount of orcine-positive compounds is released which cannot be adequate to the RNA content. Therefore the separate RNA determination in the presence of DNA was excluded even after the NA fractionation by hydrolysis at various temperature and perchloric acid concentration. Besides NA hydrolysates contained a high amount of other compounds absorbing in the UV-region....
Some properties of the virus causing the mosaic of sweet peas in Czechoslovakia
J. Polák, J. Chod
Biologia plantarum 1968, 10:424-434 | DOI: 10.1007/BF02920985
Identification trials were carried out to determine what virus causes a mosaic disease of sweet peas in Czechoslovakia. The found properties of the identified sweet pea mosaic virus, the character of its transmission and its host range prove that sweet peas in Czechoslovakia are attacked by common pea mosaic virus (CPMV). Some insignificant differences in properties between our virus isolate and CPMV were observed in the course of the determination of properties of our virus isolate on various host plants. The possible existence of more strains of CPMV is discussed. The transmission of sweet pea mosaic to the plants ofPhaseolus vulgaris L. was...
Frequency of polyvalents in relation to fertility of autogamized autotetraploid kohlrabi (Brassica oleracea var.gongylodes L.), cabbage (Brassica oleracea var.capitata L.) and their hybrids
Božena Zdráhalová
Biologia plantarum 1968, 10:435-444 | DOI: 10.1007/BF02920986
The present work is a study of the frequency of polyvalents in autotetraploid kohlrabi, cabbage and their intervarietal hybrids. Polyvalents were stated in the final phases of heterotypic prophasis, usually in diakinesis.
In none of both varieties any significant relationship between frequency of polyvalents and fertility expressed by mean number of seeds per siliqua was found, while in intervarietal hybrids some correlation was observed between mentioned characters. This leads to the conclusion that the frequency of polyvalents is not always a measure of the degree of fertility in auto-polyploids.
Autotetraploid intervarietal hybrids were characterized...
Mechanical rigidity and lignine content in different developmental phases of winter rape siliqua
V. Kratochvíl
Biologia plantarum 1968, 10:445-448 | DOI: 10.1007/BF02920987
The lignine content and mechanical rigidity of siliquae ofBrassica napus L. ssp.oleifera f.biennis, cv. Třebíčská were studied. Experimental plants were harvested in five consecutive periods and developmental stages of siliquae were determined on the basis of the weight of 1000 seeds. During the development of siliquae a gradual accumulation of lignine takes place; the content reached its maximum in certain developmental phases and then decreased. The mechanical rigidity of siliquae had a similar tendency. There were statistically significant differences in both properties studied within individual developmental phases. A highly...
The antagonism of (2-Chlorethyl) trimethylammonium chloride and indole 3-acetic acid in epicotyl and axillary buds growth in the pea seedlings
J. Šebánek
Biologia plantarum 1968, 10:449 | DOI: 10.1007/BF02920988
The 0.03-0.06% indole-3-acetic acid (IAA) in lanoline paste smeared on the cut surface of decapitated epicotyl of the pea seedling inhibited the growth of the axillary buds of the cotyledons. 0.03-0.06% solution of (2-Chlorethyl) trimethylammonium chloride (CCC) considerably weakened the inhibitory effect of IAA when applied simultaneously to the roots. In a similar way CCC diminishes even the growth inhibition of intact pea epicotyls caused by 0.06% IAA lanoline paste. 0.03% solution of CCC administered to the roots of the decapitated pea seedlings significantly increased growth of the axillary buds. This effect may play a role in the demonstrated...
Contribution to the exodermis in the rootlets ofFraxinus excelsior L.
Jarmila Kubíková
Biologia plantarum 1968, 10:455-461 | DOI: 10.1007/BF02920989
The exodermis of ash roots is initiated early in the apical meristem. When fully differentiated, it is composed of alternating "long" and "short" cells measuring approx. 70×25×25 μm and 25×28×25 μm respectively. At a short distance from the apex, the long cells undergo structural and histochemical changes from a "primary" towards a "secondary" stage: an impermeable suberized lamella is formed, cellulose lamellae become impregnated by lignin, and the protoplast dies off. The short cells show a distinctly thickened outer wall ("cap") which is composed exclusively of cellulose, and possess abundant cytoplasm with a large nucleus. In the process...
Dynamics of amino acids in pea stem sections during root formation and its inhibition by kinetin and ethionine
M. Kamínek
Biologia plantarum 1968, 10:462 | DOI: 10.1007/BF02920990
An investigation was conducted to study the interrelation of free amino acid metabolism and root formation in etiolated pea stem sections as dependent on time and on inhibition of root formation by kinetin and ethionine.
The rise in the level of aspartic acid and increase in the rate of conversion of14C-labeled glucose to free amino acids were found to be characteristic features of the formation of foci of meristematic cells in pericyclo region. The formation of roots was reflected, in general, much more in the rate of conversion of labeled glucose to free amino acids than in the levels of corresponding amino acids.
The total amount...