Biologia plantarum 2015, 59:325-330 | DOI: 10.1007/s10535-015-0502-5

Anthocyanin accumulation rate and the biosynthesis related gene expression in Dioscorea alata

J. M. Yin1,*, R. X. Yan1, P. T. Zhang1, X. Y. Han1, L. Wang1
1 Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, P.R. China

In this study, the anthocyanin content and real-time quantitative expression of the anthocyanin biosynthesis-related genes were investigated in leaves, stems, and tubers of purple yam (Dioscorea alata L.). The anthocyanin content, its accumulation, and the expression of genes encoding phenylalanine ammonia lyase (PAL), flavanone-3-hydroxylase (F3H), anthocyanidin synthase (ANS), and UDP-glycose flavonoid glycosyl transferase (UFGT) were studied. The anthocyanin content in the leaves and stems was high at early stages of growth, but it decreased and remained at a similar level from the 35th day onward. The anthocyanin content in the tubers firstly increased, reached a high peak at the 110th day of growth, after which decreased. Anthocyanin accumulation rates and the expressions of the anthocyanin biosynthesis genes were high at the early stages of growth in the leaves and stems, but in tubers, two peaks were observed: at days 80 and 140 for the gene expression and at days 125 and 170 for the anthocyanin accumulation rate. Thus, there was coordination between the gene expressions and the anthocyanin accumulation rates in the various organs as well as in the entire plants.

Keywords: anthocyanidin synthase; flavanone 3-hydroxylase; phenylalanine ammonia lyase; plant ontogeny; purple yam; UDP-glycose flavonoid glycosyl transferase
Subjects: anthocyanin accumulation; anthocyanin biosynthesis-related genes; plant ontogeny; gene expression; purple yam
Species: Dioscorea alata

Received: May 28, 2014; Revised: October 11, 2014; Accepted: October 30, 2014; Published: June 1, 2015Show citation

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Yin, J.M., Yan, R.X., Zhang, P.T., Han, X.Y., & Wang, L. (2015). Anthocyanin accumulation rate and the biosynthesis related gene expression in Dioscorea alata. Biologia plantarum, 59(2), 325-330. doi: 10.1007/s10535-015-0502-5.
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