Biologia plantarum 2015, 59:106-114 | DOI: 10.1007/s10535-014-0465-y

Responses of two barley cultivars differing in their salt tolerance to moderate and high salinities and subsequent recovery

K. Kosová1,*, P. Vítámvás1, I. Hlaváčková2, M. O. Urban1, E. Vlasáková1, I. T. Prášil1
1 Department of Plant Genetics, Breeding and Crop Quality, Crop Research Institute, Prague, Czech Republic
2 Department of Biochemistry and Microbiology, Institute of Chemical Technology Prague, Prague, Czech Republic

Two barley (Hordeum vulgare L.) cultivars, Czech spring cv. Amulet and Syrian landrace Tadmor, were subject to different salinity treatments: 1) the NaCl concentration was gradually increased from 0 (the control) to either 100 (a moderate salt stress) or 300 mM NaCl (a high salt stress), 2) the NaCl concentration was increased directly either from 0 to 300 mM NaCl or from 100 to 300 mM NaCl, and 3) a recovery when all variants were transferred back to control conditions and cultivated for seven additional days before sampling. The following parameters were determined: water saturation deficit (WSD), osmotic potential (ψs), leaf proline content, maximum quantum yield of photosystem (PS) II photochemistry (measured as variable to maximum chlorophyll a fluorescence ratio, Fv/Fm), and relative accumulation of dehydrins (DHN). Both quantitative and qualitative differences in dehydrins were found between NaCl-treated Amulet and Tadmor. A principal component analysis (PCA) of all experiment data revealed a differential ability of Amulet and Tadmor to recover after the 300 mM NaCl treatments indicating better salt tolerance in Tadmor. Correlation analyses have shown statistically significant correlations between WSD, ψs, proline, and DHN.

Keywords: chlorophyll fluorescence; dehydrins; Hordeum vulgare; proline; osmotic potential; principal component analysis
Subjects: salt tolerance; relative water content; osmotic potential; proline; chlorophyll fluorescence; dehydrin; barley; principal component analysis
Species: Hordeum vulgare

Received: April 10, 2014; Revised: August 11, 2014; Accepted: August 12, 2014; Published: January 1, 2015Show citation

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Kosová, K., Vítámvás, P., Hlaváčková, I., Urban, M.O., Vlasáková, E., & Prášil, I.T. (2015). Responses of two barley cultivars differing in their salt tolerance to moderate and high salinities and subsequent recovery. Biologia plantarum, 59(1), 106-114. doi: 10.1007/s10535-014-0465-y.
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