Translocation of leaf-fed ³²P in pea plants as influenced by foliar and root applications of CCC and Phosfon

N. O. Adedipe

Biologia plantarum 1975, 17:161-166 | DOI: 10.1007/BF02920828

In controlled environment growth chambers, the effects of foliar and root applications of 2-chloroethyltrimethylammonium chloride (CCC) and 2,4-dichlorobenzyltributylphosphonium chloride (Phosfon) on the translocation of³²P fed to leaves, were investigated. When applied to leaves or to root, CCC had no effect on the relative amounts of³²P radioactivity retained by the fed leaf 5, 20 and 80 h after feeding. At 20 and 80 h after feeding, Phosfon concentrations of 0.01 and 0.1mg l^-1 reduced retention of the applied³²P. 80 h after³²P feeding, CCC concentration of 1 mg l^-1 applied as a foliar...

Effects of different 2,4-D concentrations on the cytogenetic behaviour of plant cells culturedin vitro

B. D. Singh, B. L. Harvey

Biologia plantarum 1975, 17:167-174 | DOI: 10.1007/BF02920829

Cytogenetic effects of different concentrations of 2,4-D on suspension cultures ofVicia hajastanaGrossh. andHaplopappus gracilis(Nutt.) Gray were studied using a simple, chemically defined medium. InVicia, the frequency of dicentric chromosomes showed a negative association with the 2,4-D concentration. In both the species, the frequencies of anaphase bridges were negatively associated, while those of the diploid cells showed a positive association with the 2,4-D concentration in the culture medium. A decrease in the frequency of diploid cells was observed in the cultures transferred to a lower concentration of 2,4-D. This decrease...

Production of plant growth substances by rhizosphere mycoflora of broad bean and cotton

Y. A. Youssef, A. T. Mankarios

Biologia plantarum 1975, 17:175-181 | DOI: 10.1007/BF02920830

The culture filtrates of the rhizosphere fungi of broad bean (Vicia faba L.) and cotton (Gossypium barbadense L.) were analysed for the presence of plant growth substances of auxin and gibberellins nature. Bioassay test and chromatographic analysis indicated that these fungi, each synthesized different auxins in their culture medium. These auxins were indole compounds. Similarly the rhizosphere fungi produced in their culture medium some gibberellins and gibberellin-like substances.

Linkage relationships of recessive chlorophyll mutations in Arabidopsis thaliana (L.)Heynh

Helena Fischerová

Biologia plantarum 1975, 17:182-188 | DOI: 10.1007/BF02920831

A new method enabling to localize recessive alleles controling lethal embryonal or chlorophyll mutations in linkage groups has been devised and verified. The information on the linkage was obtained in B₁ in repulsion after the crosses with recessive visible markers representing the individual linkage groups. The distinction of four B₁ genotypes was achieved by means of Müller's embryotest. Altogether eight mutant alleles were localized. The allelesch 2411, ch 4062 andX 3 are carried by the first linkage group, the allelesM 33 andM 25 by the third and the allelech 1378 by the fourth linkage group....

A comparative study of the effect of morphactin and Niagara on the leaf epidermis

Nilima Paliwal, Bhaskar Barma, G. S. Paliwal

Biologia plantarum 1975, 17:189-197 | DOI: 10.1007/BF02920832

Treatment of two-week-oldBrassica campestris andTrigonella foenum-graecum plants with morphactin andVicia faba, Antirrhinum orontium, andPapaver somniferum with Niagara, induced marked variations in the orientation and ontogeny of stomata and the epiderma cells. Morphactin-chlorflurenol at 12.5, 62.5, 125, 250, and 500 ppm, caused marked damage of the shoot apices and changes in the epidermal tissue, such as divisions of the guard cells, reduction in the size of the stomata, and epidermal cells. Niagara-ethyl-hydrogen-1-propylphosphonate at 100, 500, 1 000 5 000, and 10 000 ppm caused thickenings of the epidermal cell walls...

Role of abscisic acid in the closure of stomata in some arid zone plant species

D. N. Sen, D. D. Chawan

Biologia plantarum 1975, 17:198-201 | DOI: 10.1007/BF02920834

Initially closed stomata in isolated leaf epidermal peelings ofCalotropis procera, Prosopis cineraria andTephrosia purpurea, opened when incubated in distilled water in light or in darkness. Water-induced open stomata closed down on incubation in abscisic acid (0.01 ppm) solution within half an hour. Sodium chloride induced the opening of stomatal aperture. NaCl-induced open stomata also completely closed down when incubated in abscisic acid solution, but open again when transferred to NaCl.

Recovery from ethyl methanesulphonate-induced genetical damage in barley. Independence of sodium azide treatment

T. Gichner, Jiřina Švachulová, J. Velemínský, V. Pokorný

Biologia plantarum 1975, 17:202-206 | DOI: 10.1007/BF02920835

Barley seeds were treated for 3 h at 25°C with 240 mM ethyl methanesulphonate (EMS), washed for 18 h, treated with various concentrations of unbuffered sodium azide (pH 6.7-7.3) for 3 h at 25°C, re-dried to 30% water content and either sown immediately or stored at 25°C for 12 days and then sown. The synergistic action of sodium azide post-treatment has been demonstrated only for the EMS-induced M₁ germination reduction, while the EMS-induced M₁ sterility and the yield of M₂ chlorophyll mutants were unaffected. The "storage" recovery from EMS-induced mutagenic effects was insensitive to sodium azide post-treatment....

Indolylacetic acid oxidase in dormant apple embryos

Katarzyna Dziewanowska, St. Lewak

Biologia plantarum 1975, 17:207-213 | DOI: 10.1007/BF02920836

The IAA oxidase activity was studied during the culture of dormant apple embryos. The effect of different factors on this enzyme activity was investigated either by adding them to the reaction mixture or to the culture medium. Phloridzin was found to be the best phenolic cofactor. The development of IAA oxidase activity was stimulated by phloridzin and GA₃. The properties of apple embryos IAA oxidase allow to postulate the presence of two enzyme systems able to oxidize IAA in the material studied. The involvement of peroxidase activity in IAA oxidation was also investigated. The differences in the changes of peroxidase and IAA oxidase activities...

Invaginations in plant cell protoplasts containing mycoplasma-like bodies (MLO)

O. Králík, J. Brčák

Biologia plantarum 1975, 17:214-218 | DOI: 10.1007/BF02920837

MLO containing invaginations were found in protoplasts of phloem parenchyma cells in symptomless young leaves ofRibes houghtonianumJancz. infected with a yellows disease. The invaginations originate between the cell wall and plasmalemma, usually at plasmodesmata, and change apparently into superficial vesicles in the protoplast; they are entirely or partially limited by host plasmalemma. The formations mentioned occur in parenchyma cells which contain normal organelles. Sometimes they are divided by a smooth membrane system enclosing MLO. Besides MLO the invaginations contain in some cases slimy fibrils resembling the P-protein in sieve tubes....

Kozubov, G. M., Danilova, M. F. (ed.): Atlas ultrastruktury rastiteInykh kletok [Atlas der ultrastruktur von pflanzenzellen]

Ingrid Tichá

Biologia plantarum 1975, 17:197 | DOI: 10.1007/BF02920833

Shul'gin, I. A.:Rastenie i Solntse [Die Pflanze und die Sonne]

Ingrid Tichá

Biologia plantarum 1975, 17:218 | DOI: 10.1007/BF02920838

Burns, G. W.:The plant Kingdom

Inorid Tichá

Biologia plantarum 1975, 17:230 | DOI: 10.1007/BF02920843

Moore, T. C.: Research experiences in plant physiology. A laboratory manual

Ingrid Tichá

Biologia plantarum 1975, 17:234 | DOI: 10.1007/BF02920845

Isolation of the protoplasts fromAsteraceae by means of xylonase

Běla Landová, Z. Landa

Biologia plantarum 1975, 17:219-222 | DOI: 10.1007/BF02920839

The enzyme xylonase was used to isolate the protoplasts from the leaves ofCalendula officinalis L.,Gazania splendensMoore,Tithonia rotundifoliaBlake,Zinnia elegansJacq- and from the petals ofDahlia variabilis(Willd.) Desf. The recovery of spherical undamaged protoplasts differed. The same method did not lead to the isolation of the protoplasts from callus cultures derived fromCalendula, Gazania andTithonia leaves respectively.

The effect of calcium deficiency on nitrate absorption and assimilation in pumpkin plants

I. Prášil

Biologia plantarum 1975, 17:223-225 | DOI: 10.1007/BF02920840

The absorption of nitrate and the activity of nitrate reductase were much lower in Ca-deficient plants ofCururbita pepo L., cv. 'Kveta' than in normal plants grown in complete nutrient solution for a period of 8 days. After the addition of nitrate to the nutrient medium, nitrate reductase activity in the roots of NO₃-deficient plants sharply rose during the first 6 h and then remained constant during the following 6 h; the content of endogenous NO₃^- rose slowly and continuously. These processes were depressed in (Ca, NO₃)-deficient plants independently of the addition of Ca²⁺ to the medium...

Etiology of two virus diseases of lilac (Syringa vulgaris L.) occurring in Czechoslovakia

J. B. Novák, Julie Lanzová

Biologia plantarum 1975, 17:226-227 | DOI: 10.1007/BF02920841

Virus origin of lilac ringspot and chlorotic ringspot of lilac was identified serologically by means of double gel diffusion method. The former of these diseases is due to Arabis mosaic virus, the latter to a mixture of Arabis mosaic virus and cherry leaf roll virus. The occurrence of these viruses has been detected for the first time in Czechoslovakia.

Regulation of glutamate dehydrogenase level in excised pea roots by some exogenously supplied compounds with auxin activity

J. Sahulka, Alena Gaudinová

Biologia plantarum 1975, 17:228-230 | DOI: 10.1007/BF02920842

Besides IAA, other compounds with auxin activity (of both indole and non-indole nature) can also cause an increase in glutamate dehydrogenase level in excised pea roots. The effect of these substances is probably not mediated by ethylene, depends on proteosynthesis, is independent of the regulatory effect of sugars and need not be influenced by the antagonism between auxins and cytokinins.

Flower promoting effect of 2-chloroethylphosphonic acid in apple trees

S. Schmidt, M. Katzfuss, T. Buban

Biologia plantarum 1975, 17:231-234 | DOI: 10.1007/BF02920844

Experiments with the growth regulator ethephon (2-chloroethylphosphonic acid) were made to stimulate the flower differentiation in the 'Carola' and 'Golden Delicious' apple cultivars in Pillnitz (GDR) and 'Jonathan' and 'Golden Delicious' in Ujfehértó (Hungaria). A twofold application of 150 ppm ethephon in June proved most effective. Our results indicate that it is possible to promote flower differentiation in apple trees without growth retardation and fruit thinning.

Peroxidase, polyphenol oxidase and catalase isoenzymes during germination and early plant development of tall and dwarf wheats (Triticum aestivum L.)

R. Singh, D. Singh

Biologia plantarum 1975, 17:235-240 | DOI: 10.1007/BF02920846

Isoenzyme pattern of peroxidase, polyphenol oxidase and catalase at different intervals during germination and in different plant parts during early development of 'C-306' and 'Hira' cultivars, representing tall and dwarf wheats, respectively were investigated. The number of isoenzymic components of the three enzymes increased progressively with the concomitant increase in the stage of germination. The isoenzymic patterns, in general, were more clear and better developed during the later stages of germination. Different tissues or organs of the wheat plant possessed specific isoenzymic pattern of these enzymes. Differences between cultivars were observed...