Biologia plantarum 2012, 56:135-139 | DOI: 10.1007/s10535-012-0029-y

Transformation with a gene for myo-inositol O-methyltransferase enhances the cold tolerance of Arabidopsis thaliana

B. Zhu1,2, R. -H. Peng1, A. -S. Xiong1, J. Xu3, X. -Y. Fu1, W. Zhao1, X. -F. Jin1, X. -R. Meng4, J. -J. Gao3, R. Cai2,*, Q. -H. Yao1,*
1 Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, P.R. China
2 College of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P.R. China
3 College of Horticulture, Nanjing Agricultural University, Nanjing, P.R. China
4 College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, P.R. China

In this study, we report a function of myo-inositol-O-methyltransferase (Imt1) in response to low temperature stress using transgenic Arabidopsis thaliana. Imt1 gene was constructed identical to the Imt1 gene from a halophyte Mesembryanthemum crystallinum. After cold stress, the Imt1 transgenic plants exhibited stronger growth than the wild type plants. The elevated cold tolerance of the Imt1 over-expressing plants was confirmed by the lower electrolyte leakage and accumulation of malondialdehyde, but higher proline and soluble sugar contents in transgenic than wild type plants.

Keywords: electrolyte leakage; malondialdehyde; proline; soluble sugars
Subjects: electrolyte leakage; malondialdehyde; proline; myo-inositol O-methyltransferase; temperature - low; PCR; gene construct; sugars
Species: Arabidopsis thaliana

Received: July 31, 2010; Accepted: October 15, 2010; Published: March 1, 2012Show citation

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Zhu, B., Peng, R.-H., Xiong, A.-S., Xu, J., Fu, X.-Y., Zhao, W., ... Yao, Q.-H. (2012). Transformation with a gene for myo-inositol O-methyltransferase enhances the cold tolerance of Arabidopsis thaliana. Biologia plantarum, 56(1), 135-139. doi: 10.1007/s10535-012-0029-y.
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