Biologia plantarum 2012, 56:493-501 | DOI: 10.1007/s10535-012-0057-7

Ectopic expression of peanut acyl carrier protein in tobacco alters fatty acid composition in the leaf and resistance to cold stress

G. -Y. Tang1,2,3, L. -Q. Wei4, Z. -J. Liu1,2,3, Y. -P. Bi1,2,3, L. Shan1,2,3,*
1 High-Tech Research Center, Shandong Academy of Agricultural Sciences, Jinan, P.R.China
2 Key Laboratory for Genetic Improvement of Crop, Animal and Poultry of Shandong Province, Jinan, P.R.China
3 Key Laboratory of Crop Genetic Improvement and Biotechology, Huanghuaihai, Ministry of Agriculture, Jinan, P.R.China
4 Life Sciences College, Shandong Normal University, Jinan, P.R. China

Acyl carrier protein (ACP), as an essential protein cofactor, plays an important role in de novo synthesis of fatty acids in plastids. In this study, the expression profile of peanut (Arachis hypogaea) AhACP1-1 and AhACP1-2 was analyzed in different tissues. The expression level of AhACP1-1 was highest in the seed, whereas expression was barely detected in the shoot, and AhACP1-2 was expressed in every tissue analyzed with the highest expression level detected in the leaf and seed. Overexpression (OE) and antisense-inhibition (AT) of AhACP1 in transgenic tobacco modified the transcript level of endogenous NtACPs, and the content of total lipids and composition of fatty acid in leaves were altered compared with the wild-type control. Transgenic OE-AhACP1 or AT-AhACP1 tobacco exhibited a significant increase or decrease in polyunsaturated C18:2 and C18:3 fatty acid content, and were more tolerant or sensitive to cold stress, respectively. It is suggested that AhACP1 bound with C18:1 might be the specific substrate of oleoyl-ACP thioesterase or glycerol-3-phosphate acyltransferase, and participates in membrane lipid synthesis.

Keywords: antisense; Arabidopsis thaliana; Nicotiana tabacum; overexpression; transgenic tobacco
Subjects: antisense; overexpression; transgenic plants; acyl carrier protein; fatty acids; lipids; chlorophyll fluorescence; nucleotide sequence; phylogenetic tree; malondialdehyde
Species: Nicotiana tabacum; Arachis hypogaea

Received: March 29, 2011; Accepted: June 20, 2011; Published: September 1, 2012Show citation

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Tang, G.-Y., Wei, L.-Q., Liu, Z.-J., Bi, Y.-P., & Shan, L. (2012). Ectopic expression of peanut acyl carrier protein in tobacco alters fatty acid composition in the leaf and resistance to cold stress. Biologia plantarum, 56(3), 493-501. doi: 10.1007/s10535-012-0057-7.
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