Biologia plantarum 2012, 56:705-710 | DOI: 10.1007/s10535-012-0244-6

Cytosolic NADP-isocitrate dehydrogenase in Arabidopsis leaves and roots

M. Leterrier1, J. B. Barroso2, J. M. Palma1, F. J. Corpas1,*
1 Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Granada, Spain
2 Grupo de Señalización Molecular y Sistemas Antioxidantes en Plantas, Unidad Asociada al CSIC, Departamento de Bioquímica y Biología Molecular, Universidad de Jaén, Jaén, Spain

NADP-dependent isocitrate dehydrogenase (NADP-ICDH) catalyses the production of NADPH, which is an essential component in the cellular homeostasis. In Arabidopsis, the kinetic parameters (K m and V max) of cytosolic NADP-ICDH were different in leaves and roots. In vitro applied H2O2 did not affect the NADP-ICDH activity in either organ, however, the reduced glutathione inhibited the activity in leaves but not in roots. On the other hand, S-nitrosoglutathione (a NO donor) and peroxynitrite depressed NADP-ICDH activity in leaves and roots.

Keywords: hydrogen peroxide; nitric oxide; peroxynitrite; reactive nitrogen species; S-nitrosoglutathione
Subjects: NAPD-isocitrate dehydrogenase; hydrogen peroxide; nitric oxide; peroxynitrite; reactive oxygen species; nitrosoglutathione; cellular homeostasis; citrate
Species: Arabidopsis thaliana

Received: September 2, 2011; Accepted: November 11, 2011; Published: December 1, 2012Show citation

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Leterrier, M., Barroso, J.B., Palma, J.M., & Corpas, F.J. (2012). Cytosolic NADP-isocitrate dehydrogenase in Arabidopsis leaves and roots. Biologia plantarum, 56(4), 705-710. doi: 10.1007/s10535-012-0244-6.
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