Biologia plantarum 2015, 59:316-324 | DOI: 10.1007/s10535-015-0487-0

Identification of a putative stearoyl acyl-carrier-protein desaturase gene from Saussurea involucrata

H. -L. Liu1,2,*, H. -T. Shen1, C. Chen1, X. -R. Zhou3, H. Liu4,*, J. -B. Zhu1,*
1 Laboratory of Agricultural Biotechnology, College of Life Science of Shihezi University, Shihezi, Xinjiang, P.R. China
2 Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai, P.R. China
3 CSIRO Plant Industry, Canberra, Australia
4 China ColoredCotton Group Co., Urumqi, P.R. China

Saussurea involucrata Kar. et Kir. tolerates severe abiotic stresses including cold, and the level of membrane fatty acid desaturation is associated with its cold acclimation. We discovered and characterized a full-length cDNA of stearoyl acyl-carrier-protein desaturase (sikSACPD) which encodes a protein consisted of 396 amino acids. A sequence alignment of the SikSACPD protein showed that it shares 91 and 86 % identity with the SACPDs of Carthamus tinctorius and Helianthus annuus, respectively. Semi-quantitative RT-PCR showed that the expression of sikSACPD increased in S. involucrata leaves as the temperature decreased from 20 to -10 °C. Agrobacterium tumefaciens was used to transform fatty acid biosynthesis 2 (FAB2):SikSACPD and FAB2:FAB2 constructs into tobacco to investigate resistance to a freezing stress and fatty acid composition of the transgenic plants. The FAB2:SikSACPD transgenic plants showed a slightly more resistance to the freezing stress than the FAB2:FAB2 transgenic plants and the wild-type. The proportion of oleic acid (C18:1) in the leaves of SikSACPD transgenic tobacco increased from approximately 5 to 20 % compared with the leaves of non-transgenic tobacco when both were exposed to cold stress treatments. This study demonstrates that the SikSACPD transgene, when expressed in tobacco, conferred a higher cold tolerance in comparison with that observed in non-transgenic tobacco. Thus, this gene may be a candidate for enhancing cold tolerance in other crop plants.

Keywords: Agrobacterium tumefaciens; cold resistance; fatty acids; Nicotiana tabaccum; sikSACPD; transgenic plants
Subjects: stearoyl acyl-carrier-protein; fatty acids; desaturase; transgenic plant; cold tolerance; temperature; malondialdehyde; electrolyte leakage; chlorophyll fluorescence
Species: Saussurea involucrata; Nicotiana nudicaulis; Agrobacterium tumefaciens

Received: February 19, 2014; Revised: June 27, 2014; Accepted: July 9, 2014; Published: June 1, 2015Show citation

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Liu, H.-L., Shen, H.-T., Chen, C., Zhou, X.-R., Liu, H., & Zhu, J.-B. (2015). Identification of a putative stearoyl acyl-carrier-protein desaturase gene from Saussurea involucrata. Biologia plantarum, 59(2), 316-324. doi: 10.1007/s10535-015-0487-0.
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    References

    1. Carmak, I., Horst, J.H.: Effect of aluminium on lipid peroxidation, SOD, CAT and peroxidase activities in root tips of soybean. - Physiol. Plant. 83: 463-468, 1991. Go to original source...
    2. Chen, F.J., Yang, Y.G., Zhao, D.X.: Advances in studies of species, habitats distribution and chemical composition of snow lotuses (Saussurea) in China. - Chin. Bull. Bot. 16: 561-566, 1999.
    3. Chen, J.M., Yu X.P., Cheng, J.A.: The application of chlorophyll fluorescence kinetics in the study of physiological responses of plants to environmental stresses. - Acta. agr. zhejiangensis 18: 51-55, 2006.
    4. Chen, X.B., Zhang, A.P., Yao, Q.H.: Research advances on gene engineering of plant for cold resistance. - Lett. Biotechnol. 12: 318-323, 2001.
    5. Dellaporta, S.L., Wood, J., Hicks, J.B.: A plant DNA miniprepration version II. - Plant mol. Biol. Rep. 1: 19-21, 1983. Go to original source...
    6. Doyle, J.J., Doyle, J.L.: Isolation of plant DNA from fresh tissue. - Focus 12: 13-15, 1990.
    7. Gao, S.H., Guo, J. P., Zhang, G.M., Wang, L.M., Wang, L.Z.: The impacts of low temperature on corn seedling physiological response. - Quart. J. appl. Meteorol. 10: 238-242, 1999.
    8. Gong, Z., Lee, H., Xiong, L., Jagendorf, A., Stevenson, B., Zhu, J.K.: RNA helicase-like protein as an early regulator of transcription factors for plant chilling and freezing tolerance. - Proc. nat. Acad. Sci. USA. 99: 11507-11512, 2002. Go to original source...
    9. Guo, X.Y., Chen, C., Wang, Z., Wang, H.L., Yu, N., Wang, A.Y., Zhu, J.B.: Cold resistance identification of tobacco plants transformed by siCOR of Saussurea involucrata Kar. et Kir. - Acta. bot. boreal-occident. sin. 32: 1-10, 2012.
    10. Guo, Y., Xiong, L., Ishitani, M., Zhu, J.K.: An Arabidopsis mutation in translation elongation factor 2 causes super-induction of CBF/DREB1 transcription factor genes but blocks the induction of their downstream targets under low temperature. - Proc. nat. Acad. Sci. USA. 99: 7786-7791, 2002. Go to original source...
    11. He, H.Y., Tian, L.P., Xue, L.: Research progress on physiology and biochemistry of plant cold hardiness. - Tianjin agr. Sci. 13: 10-13, 2007.
    12. Hirayama, T, Shinozaki, K.: Research on plant abiotic stress responses in the post-genome era: past, present and future. - Plant J. 61:1041-1052, 2010. Go to original source...
    13. Huang, J.H., Tan, D.Y.: Advances in studies of snow lotuses (Saussurea). - J. Xinjiang. Agr. Univ. 25: 8-13, 2002.
    14. Ishitani, M., Xiong, L., Lee, H., Stevenson, B., Zhu, J.K.: HOS1, a genetic locus involved in cold-responsive gene expression in Arabidopsis. - Plant Cell 10: 1151-1161, 1998. Go to original source...
    15. Knutzon, D.S., Thompson, G.A., Radke, S.E., Johnson, W.B., Knauf, V.C., Kridl, J.C.: Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene. - Proc. nat. Acad. Sci. USA. 89: 2624-2628, 1992. Go to original source...
    16. Kodama, H., Hamada, T., Horiguchi, G., Nishimura, M., Iba, K.: Genetic enhancement of cold tolerance by expression of a gene for chloroplast ω-3 fatty acid desaturases in transgenic tobacco. - Plant Physiol. 105: 601-605, 1994. Go to original source...
    17. Kodama, H., Horiguchi, G., Nishiuchi, T., Nishimura, M., Iba, K.: Fatty acid desaturation during chilling acclimation is one of the factors involved in conferring low temperature tolerance to young tobacco leaves. - Plant Physiol. 107: 1177-1185, 1995. Go to original source...
    18. Los, D., Horvath, I., Vigh, L., Murata, N.: The temperature-dependent expression of the desaturase gene desA in Synechocystis PCC6803. - FEBS. Lett. 318: 57-60, 1993. Go to original source...
    19. Ma, J., Liu, D., Fu, Y.: Nucleotide sequence of a cDNA clone encoding spinach stearoyl-acyl carrier protein desaturase (SAD). - J. Agr. Biotechnol. 4: 33-37, 1996a.
    20. Ma, J., Liu, D., Tang, P.: Cloning of spinach SAD gene, its construction and transformation to tobacco. - Plant Physiol. 111(Suppl.): 814, 1996b.
    21. Mauro, S., Dainsese, P., Lannoye, R., Bassi, R.: Cold resistant and cold susceptible maize lines differ in the phosphorylation of the photosystem II subunit. - Plant Physiol. 115: 171-177, 1997. Go to original source...
    22. Maxwell, K, G.N. Johnson.: Chlorophyll fluorescence. A practical guide. - J. exp. Bot. 51: 659-668, 2000. Go to original source...
    23. McKeon, T.M., Stump, P.K.: Purification and characterization of the stearoyl acyl carrier protein desaturase and the acyl acyl carrier protein thioesterase from maturing seeds of safflower. - J. biol. Chem. 257: 12141-12147, 1992.
    24. Moellering, E.R., Muthan, B., Benning, C.: Freezing tolerance in plants requires lipid remodeling at the outer chloroplast membrane. - Science 330: 226-228, 2010. Go to original source...
    25. Nishida, I., Beppu, T., Matsuo, T., Murata, N.: Nucleotide sequence of a cDNA clone encoding a precursor to stearoyl(acyl-carrier-protein) desaturase from spinach, Spinach oleacea. - Plant mol. Biol. 19: 711-713, 1992. Go to original source...
    26. Ohlrogge, J., Browse, J.: Lipid biosynthesis. - Plant Cell 7: 957-970, 1995. Go to original source...
    27. Qiu, H.L., Zhang, L.H., Liu, C., He, L., Wang, A.Y., Liu, H.L., Zhu, J.B.: Cloning and characterization of a novel dehydrin gene, SiDhn2, from Saussurea involucrata Kar. et Kir. - Plant mol. Biol. 84: 707-718, 2014. Go to original source...
    28. Sato, A., Becker, C.K., Knauf, V.C.: Nucleotide sequence of a complementary DNA clone encoding stearoyl-acyl carrier protein desaturase from Simmondsia chinensis. - Plant Physiol. 99: 362-363, 1992. Go to original source...
    29. Severin, K., Schöffl, F.: Heat-inducible hygromycin resistance in transgenic tobacco. - Plant mol. Biol. 15: 827-33, 1990. Go to original source...
    30. Shanklin, J., Somerville, C.R.: Stearyl-acyl carrier protein desaturase from higher plants is structurally unrelated to the animal and fungal homologs. - Proc. nat. Acad. Sci. USA. 88: 2510-2514, 1991. Go to original source...
    31. Slocombe, S.P., Cummins, I., Jarvis, R.P., Murphy, D.J.: Nucleotide sequence and temporal regulation of a seedspecific Brassica napus cDNA encoding a stearoyl-acyl carrier protein (ACP) desaturase. - Plant mol. Biol. 20: 151-155, 1992. Go to original source...
    32. Taylor, M.A., Smith, S.B., Davies, H.V., Burch, L.R.: The primary structure of a cDNA clone of the stearoyl-acyl carrier protein desaturase gene from potato (Solanum tuberosaum L.). - Plant Physiol. 100: 533-534, 1992. Go to original source...
    33. Thomashow, M.F.: Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. - Annu. Rev. Plant Physiol. Plant mol. Biol. 50: 571-599, 1999. Go to original source...
    34. Thompson, G.A., Scherer, D.E., Foxall-Van Aken, S, Kenny, J.W., Young, H.L., Shintani, D.K., Kridl, J.C., Knauf, V.C.: Primary structures of the precursor and mature forms of stearoyl-acyl carrier protein desaturase from safflower embryos and requirement of ferredoxin for enzyme activity. - Proc. nat. Acad. Sci. USA. 88: 2578-2582, 1991. Go to original source...
    35. Tong, L., Shu, M.P., Wu, Y.D., Dan, W.M., Ying, X., Meng, X., Fang, C.: Characterization of a new stearoyl-acyl carrier protein desaturase gene from Jatropha curcas. - Biotechnol. Lett. 28: 657-662, 2006. Go to original source...
    36. Wada, H., Gombos, Z., Murata, N.: Enhancement of chilling tolerance of a cyanobacterium by genetic manipulation of fatty acid desaturation. - Nature 347: 200-203, 1990. Go to original source...
    37. Xin, Z., Browse, J.: Eskimo1 mutants of Arabidopsis are constitutively freezing-tolerant. - Proc. nat. Acad. Sci. USA 95: 7799-7804, 1998. Go to original source...
    38. Yan, M.L., Li X.D., Lin, Y.J., Wang, L.L., Zhou, L.Y.: Effects of drought during seedling stage on physiological traits, sin. 33: 113-119, 2007.
    39. Zhang, Q.L., Zhao, Y., Li, X.W., Zhai, Y., Zhang, Y., Wang, Y., Li, J.W., Wang, Q.Y.: Cloning and activity analysis of soybean SACPD-C promoter. - Acta. agron. sin. 37: 1205-1211, 2011. Go to original source...
    40. Zhu, J.B., Liu, H.L., Wang, Z., Zhou, P.: Construction of fulllength cDNA library from the Saussurea involucrata Kar. et Kir laminae. - Acta. agr. boreal-occident. sin. 15: 170-173, 2006.

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