Biologia plantarum 2016, 60:419-426 | DOI: 10.1007/s10535-016-0624-4

Identification and expression analysis of nuclear factor Y families in Prunus mume under different abiotic stresses

J. Yang1, X. L. Wan1, C. Guo1, J. W. Zhang1,*, M. Z. Bao1
1 Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, P.R. China

The nuclear factor Y (NF-Y) is one of the largest transcription factor families in plants consisting of NF-YA, NF-YB, and NF-YC subunits. It could play important roles in various processes such as flowering time, seed development, and response to drought. In this study, 6 NF-YA, 13 NF-YB, and 8 NF-YC proteins were identified and characterized in Prunus mume. Analyses of a conserved domain indicated that the PmNF-Y subunits shared an elevated degree of homology with the corresponding Arabidopsis NF-Y ones. Phylogenetic analysis showed that each NF-Y subunit family from Prunus mume and Arabidopsis could be divided into 4 or 2 clades based on their full-length proteins. The gene expression patterns of all 27 PmNF-Y genes were examined under abscisic acid (ABA), osmotic, salt, and H2O2 treatments using real-time quantitative PCR analyses. PmNF-YA1/2/4/5/6, PmNF-YB3/4/8/10/11/13, and PmNF-YC1/2/4/5/6/8 were found to be up-regulated under the ABA and osmotic treatments. PmNF-YA1/2/3/4/5/6, PmNF-YB1/3/8/10/11/13, and PmNF-YC1/2/5/6/8 were obviously induced by the H2O2. In addition, only PmNF-YA2 and PmNF-YB3 expressions were enhanced under the salt stress. These findings could provide an entry point to investigating the roles of PmNF-Y genes during abiotic stress responses.

Keywords: abscisic acid; Arabidopsis thaliana; gene expression; H2O2; osmotic stress; salinity
Subjects: expression analysis; transcription factor; abscisic acid; osmotic stress; salinity; phylogenetic tree; hydrogen peroxide
Species: Prunus mume; Arabidopsis thaliana

Received: August 5, 2015; Revised: December 4, 2015; Accepted: December 7, 2015; Published: September 1, 2016Show citation

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Yang, J., Wan, X.L., Guo, C., Zhang, J.W., & Bao, M.Z. (2016). Identification and expression analysis of nuclear factor Y families in Prunus mume under different abiotic stresses. Biologia plantarum, 60(3), 419-426. doi: 10.1007/s10535-016-0624-4.
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