Biologia plantarum 61:155-168, 2017 | DOI: 10.1007/s10535-016-0642-2

Effects of nitric oxide and Fe supply on recovery of Fe deficiency induced chlorosis in peanut plants

Y. L. Song1, Y. J. Dong1,*, X. Y. Tian1, W. W. Wang1, Z. L. He2
1 College of Resources and Environment, Shandong Agricultural University, Tai'An, P.R. China
2 Institute of Food and Agricultural Sciences, University of Florida, Fort Pierce, USA

The effects of nitric oxide (NO) and/or iron (Fe) supplied to Fe deficient plants have been investigated in peanut (Arachis hypogaea L.) grown in Hoagland nutrient solution with or without Fe. Two weeks after Fe deprivation, recovery was induced by addition of 250 μM sodium nitroprusside (SNP, a NO donor) and/or 50 μM Fe (Fe-EDTA) to the Fe deprived (-Fe) nutrient solution. Activities of antioxidant enzymes, leaf chlorophyll (Chl), and active Fe content decreased, whereas activities of H+-ATPase, ferric-chelate reductase (FCR), nitrate reductase, and nitric oxide synthase and NO production increased in Fe deficient plants, consequently an Fe chlorosis symptom appeared obviously. In contrast, these symptoms disappeared gradually after two weeks with NO and/or Fe supply, which caused an increases in leaf Chl and active Fe content, especially following by co-treatment with NO and Fe to values found in Fe sufficient plants. Increased activities of antioxidant enzymes (superoxide dismutase, peroxidase, and catalase) and decreased accumulation of reactive oxygen species (H2O2, O2*- ) and malondialdehyde enhanced the ability of resistance to oxidative stress. Supplied NO alone had the obvious effect on increased NO production and on activity of H+-ATPase and FCR, whereas root length and root/shoot ratio were most effectively increased by Fe supplied alone. Co-treatment with NO and Fe did the best effects on recovery peanut chlorosis symptoms by significantly increased Chl and available Fe content and adjusted distribution of Fe and other mineral elements (Ca, Mg, and Zn) in both leaves and roots.

Keywords: active Fe; antioxidant enzymes; chlorophyll; ferric-chelate reductase; H+-ATPase; nitrate reductase; nitric oxide synthase; reactive oxygen species
Subjects: nitric oxide; iron deficiency; chlorosis; chlorophyll; ferric chelate reductase; ATPase; calcium; magnesium; zinc; iron distribution; catalase; superoxide dismutase; nitrate reductase; nitric oxide synthase; peanut

Received: October 19, 2015; Revised: January 3, 2016; Accepted: February 19, 2016; Published: January 1, 2017Show citation

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Song, Y.L., Dong, Y.J., Tian, X.Y., Wang, W.W., & He, Z.L. (2017). Effects of nitric oxide and Fe supply on recovery of Fe deficiency induced chlorosis in peanut plants. Biologia plantarum, 61(1), 155-168. doi: 10.1007/s10535-016-0642-2.
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