Biologia plantarum 2012, 56:50-56 | DOI: 10.1007/s10535-012-0015-4

Isolation and functional characterization of MxCS1: a gene encoding a citrate synthase in Malus xiaojinensis

D.-G. Han1,2, Y. Wang1, L. Zhang1, L. Ma1, X.-Z. Zhang1, X.-F. Xu1, Z.-H. Han1,*
1 Institute for Horticultural Plants, China Agricultural University and Key Laboratory of Beijing Municipality of Stress Physiology and Molecular Biology for Fruit Tree, Beijing, P.R. China
2 College of Horticulture, Northeast Agricultural University, Haerbin, P.R. China

Iron is one of the essential micronutrients required by all living organisms. In this study, we isolated a gene encoding putative citrate synthase (CS) from Malus xiaojinensis, designated as MxCS1. The MxCS1 gene encodes a protein of 473 amino acid residues with a predicted molecular mass of 52.5 kDa and a theoretical isoelectric point of 8.67. The expression of MxCS1 was enriched in the leaf rather than in phloem and root, however, its expression was hardly detected in the xylem. The gene expression was strongly induced by Fe stress treatment in the M. xiaojinensis seedlings. Over-expression of MxCS1 improved Fe deficiency tolerance in transgenic Arabidopsis. We argued that MxCS1 is a new member of the CS genes, and it may function as a regulator in response to iron stress in plants.

Keywords: gene expression; iron deficiency; transgenic Arabidopsis
Subjects: gene expression; iron deficiency; transgenic Arabidopsis; citrate synthase; benzyladenine; indolebutyric acid; RACE; PCR; nucleotide sequence; amino acid sequence; phylogenetic tree; vascular bundles; chlorophyll; citric acid
Species: Malus xiaojinensis; Arabidopsis thaliana

Received: April 13, 2010; Accepted: September 21, 2010; Published: March 1, 2012Show citation

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Han, D.-G., Wang, Y., Zhang, L., Ma, L., Zhang, X.-Z., Xu, X.-F., & Han, Z.-H. (2012). Isolation and functional characterization of MxCS1: a gene encoding a citrate synthase in Malus xiaojinensis. Biologia plantarum, 56(1), 50-56. doi: 10.1007/s10535-012-0015-4.
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