Biologia plantarum 2012, 56:157-161 | DOI: 10.1007/s10535-012-0034-1

Dehydrin and proline content in Brassica napus and B. carinata under cold stress at two irradiances

M. Klíma1,*, P. Vítámvás1, S. Zelenková2, M. Vyvadilová1, I. T. Prášil1
1 Crop Research Institute, Prague, Czech Republic
2 Faculty of Science, Charles University, Praque, Czech Republic

The accumulation of cold-induced dehydrin and proline was related to the frost tolerance (FT) in several Brassica species or cultivars. A dehydrin of molecular mass 47 kDa was detected in the leaves of an Ethiopian mustard (B. carinata) and a pair of dehydrins of similar molecular mass in the three (two winter, one spring) oilseed rape (B. napus) cultivars, when plants were maintained at 4 °C for one-month under two different irradiances. More dehydrin was accumulated in oilseed rape than in Ethiopian mustard under the high irradiance. A significant correlation was observed between leaf dehydrin content and FT, and no relationship between proline content and FT or between the proline and dehydrin contents. Protoplast-derived callus cells behaved differently from leaves sampled from intact plants, as they did not accumulate dehydrin and proline in response to cold stress.

Keywords: Ethiopian mustard; frost tolerance; oilseed rape; protein marker; protoplast-derived callus
Subjects: frost tolerance; protein marker; temperature - low; dehydrin; proline; irradiance; Western blot
Species: Brassica napus; Brassica carinata

Received: May 20, 2010; Accepted: January 31, 2011; Published: March 1, 2012Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Klíma, M., Vítámvás, P., Zelenková, S., Vyvadilová, M., & Prášil, I.T. (2012). Dehydrin and proline content in Brassica napus and B. carinata under cold stress at two irradiances. Biologia plantarum, 56(1), 157-161. doi: 10.1007/s10535-012-0034-1.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Atici, Ö., Demir, Y., Kocaçalişkan, I.: Effects of low temperature on winter wheat and cabbage leaves. - Biol. Plant. 46: 603-606, 2003. Go to original source...
    2. Bhattarai, T., Fettig, S.: Isolation and characterization of a dehydrin gene from Cicer pinnatifidum, a drought-resistant wild relative of chickpea. - Physiol. Plant. 123: 452-458, 2005. Go to original source...
    3. Bravo, L.A., Close, T.J., Corcuera, L.J., Guy, C.L.: Characterization of an 80-kDa dehydrin-like protein in barley responsive to cold acclimation. - Physiol. Plant. 106: 177-183, 1999. Go to original source...
    4. Cansev, A., Gulen, H., Eris, A.: Cold-hardiness of olive (Olea europaea L.) cultivars in cold-acclimated and nonacclimated stages: seasonal alteration of antioxidative enzymes and dehydrin-like proteins. - J. agr. Sci. 147: 51-61, 2009. Go to original source...
    5. Deng, Z.X., Pang, Y.Z., Kong, W.W., Chen, Z.H., Wang, X.L., Liu, X.J., Pi, Y., Sun, X.F.M., Tang, K.X.: A novel ABAdependent dehydrin ERD10 gene from Brassica napus. - DNA Sequence 16: 28-35, 2005. Go to original source...
    6. Dörffling, K., Dörffling, H., Luck, E.: Improved frost tolerance and winter hardiness in proline overaccumulating winter wheat mutants obtained by in vitro-selection is associated with increased carbohydrate, soluble protein and abscisic (ABA) levels. - Euphytica 165: 545-556, 2009. Go to original source...
    7. Fowler, D.B.: Cold acclimation threshold induction temperatures in cereals. - Crop Sci. 48: 1147-1154, 2008. Go to original source...
    8. Fuller, M.P., Metwali, E.M.R., Eed, M.H., Jellings, A.J.: Evaluation of abiotic stress resistance in mutated populations of cauliflower (Brassica oleracea var. botrytis). - Plant Cell Tissue Organ Cult. 86: 239-248, 2006. Go to original source...
    9. Ganeshan, S., Vítámvás, P., Fowler, D.B., Chibbar, R.N.: Quantitative expression analysis of selected COR genes reveals their differential expression in leaf and crown tissues of wheat (Triticum aestivum L.) during an extended low temperature acclimation regimen. - J. exp. Bot. 59: 2393-2402, 2008. Go to original source...
    10. Gleeson, D., Lelu-Walter, M.-A., Parkinson, M.: Overproduction of proline in transgenic hybrid larch (Larix × leptoeuropaea (Dengler) cultures renders them tolerant to cold, salt and frost. - Mol. Breed. 15: 21-29, 2005. Go to original source...
    11. Gothandam, K.M., Nalini, E., Karthikeyan, S., Shin, J.S.: OsPRP3, a flower specific proline-rich protein of rice, determines extracellular matrix structure of floral organs and its overexpression confers cold-tolerance. - Plant mol. Biol. 72: 125-135, 2010. Go to original source...
    12. Gray, G.R., Chauvin, L.P., Sarhan, F., Huner, N.P.A.: Cold acclimation and freezing tolerance. A complex interaction of light and temperature. - Plant Physiol. 114: 467-474, 1997. Go to original source...
    13. Ingram, J., Bartels, D.: The molecular basis of dehydration tolerance in plants. - Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 377-403, 1996. Go to original source...
    14. Janáček, J., Prášil, I.: Quantification of plant frost injury by nonlinear fitting of an S-shaped function. - Cryo-Letters 12: 47-52, 1991.
    15. Janda, T., Szalai, G., Leskó, K., Yordanova, R., Apostol, S., Popova, L.: Factors contributing to enhanced freezing tolerance in wheat during frost hardening in the light. - Phytochemistry 68: 1674-1682, 2007. Go to original source...
    16. Janská, A., Zelenková, S., Klíma, M., Vyvadilová, M., Prášil, I.T.: Freezing tolerance and proline content of in vitro selected hydroxyproline resistant winter oilseed rape. - Czech J. Genet. Plant Breed. 46: 35-40, 2010. Go to original source...
    17. Jiménez-Bremont, J.F., Becerra-Flora, A., Hernandéz-Lucero, E., Rodriguéz-Kessler, M., Acosta-Gallegos, J.A., Ramiréz-Pimentel, J.G.: Proline accumulation in two bean cultivars under salt stress and the effect of polyamines and ornithine. - Biol. Plant. 50: 763-766, 2006. Go to original source...
    18. Kaur, N.D., Vyvadilová, M., Klíma, M., Bechyně, M.: A simple procedure for mesophyll protoplast culture and plant regeneration in Brassica oleracea L. and Brassica napus L. - Czech J. Genet. Plant Breed. 42: 103-110, 2006.
    19. Kavi Kishor, P.B., Sangam, S., Amrutha, R.N., Sri Laxmi, P., Naidu, K.R., Rao, K.R.S.S., Rao, S., Reddy, K.J., Theriappan, P., Sreenivasulu, N.: Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. - Curr. Sci. 88: 424-438, 2005.
    20. Kosová, K., Holková, L., Prášil, I.T., Prášilová, P., Bradáčová, M., Vítámvás, P., Čapková, V.: The expression of dehydrin 5 during the development of frost tolerance in barley (Hordeum vulgare). - J. Plant Physiol. 165: 1142-1151, 2008. Go to original source...
    21. Kosová, K., Prášil, I.T., Prášilová, P., Vítámvás, P., Chrpová, J.: The development of frost tolerance and DHN5 protein accumulation in barley (Hordeum vulgare) doubled haploid lines derived from Atlas68 × Igri cross during cold acclimation. - J. Plant Physiol. 167: 343-350, 2010. Go to original source...
    22. Kosová, K., Vítámvás, P., Prášil, I.T.: The role of dehydrins in plant response to cold. - Biol. Plant. 51: 601-617, 2007. Go to original source...
    23. Kumar, V., Shriram, V., Kavi Kishor, P. B., Jawali, N., Shitole, M.G.: Enhanced proline accumulation and salt stress tolerance of transgenic indica rice by over-expressing P5CSF129A gene. - Plant Biotechnol. Rep. 4: 37-48, 2010. Go to original source...
    24. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. - Nature 277: 680-685, 1970. Go to original source...
    25. Li, G., Wan, S., Zhou, J., Yang, Z., Qin, P.: Leaf chlorophyll fluorescence, hyperspectral reflectance, pigments content, malondialdehyde and proline accumulation responses of castor bean (Ricinus communis L.) seedlings to salt stress levels. - Indian Crop. Prod. 31: 13-19, 2010. Go to original source...
    26. McClinchey, S.L., Kott, L.S.: Production of mutants with high cold tolerance in spring canola (Brassica napus). - Euphytica 162: 51-67, 2008. Go to original source...
    27. Parmentier-Line, C.M., Panta, G.R., Rowland, L.J.: Changes in dehydrin expression associated with cold, ABA and PEG treatments in blueberry cell cultures. - Plant Sci. 162: 273-282, 2002. Go to original source...
    28. Patton, A.J., Cunningham, S.M., Volenec, J.J., Reicher, Z.J.: Differences in freeze tolerance of zoysiagrasses. II. carbohydrates and proline. - Crop Sci. 47: 2170-2181, 2007. Go to original source...
    29. Pociecha, E., Płażek, A., Janowiak, F., Zwierzykowski, Z.: ABA level, proline and phenolic concentration, and PAL activity induced during cold acclimation in androgenic Festulolium forms with contrasting resistance to frost and pink snow mould (Microdochium nivale). - Physiol. mol. Plant Pathol. 73: 126-132, 2009.
    30. Prášil, I. T., Prášilová, P., Pánková, K.: The relationship between vernalization requirement and frost tolerance in substitution lines of wheat. - Biol. Plant. 49: 195-200, 2005.
    31. Prášil, I., Zámečník, J.: The use of a conductivity measurement method for assessing freezing injury. I. Influence of leakage time, segment number, size and shape in a sample on evaluation of the degree of injury. - Environ. exp. Bot. 40: 1-10, 1998. Go to original source...
    32. Rapacz, M.: Frost resistance and cold acclimation abilities of spring-type oilseed rape. - Plant Sci. 147: 55-64, 1999. Go to original source...
    33. Renault, J., Hoffmann, L., Hausman, J.F.: Biochemical and physiological mechanisms related to cold acclimation and enhanced freezing tolerance in poplar plantlets. - Physiol. Plant. 125: 82-94, 2005. Go to original source...
    34. Sarhan, F., Ouellet, F., Vazquez-Tello, A.: The wheat wcs120 gene family. A useful model to understand the molecular genetics of freezing tolerance in cereals. - Physiol. Plant. 101: 439-445, 1997. Go to original source...
    35. Thomashow, M.F.: Molecular genetics of cold acclimation in higher plants. - Adv. Genet. 28: 99-131, 1990. Go to original source...
    36. Toka, I., Planchais, S., Cabassa, C., Justin, A. M., Vos, D., De Richard, L., Savoure, A., Carol, P.: Mutations in the hyperosmotic stress-responsive mitochondrial basic amino acid carrier 2 enhance proline accumulation in Arabidopsis. - Plant Physiol. 152: 1851-1862, 2010.
    37. Vítámvás, P., Kosová, K., Prášilová, P., Prášil, I.T.: Accumulation of WCS120 protein in wheat cultivars grown at 9 °C or 17 °C in relation to their winter survival. - Plant Breed. 129: 611-616, 2010. Go to original source...
    38. Vítámvás, P., Prášil, I.T.: WCS120 protein family and frost tolerance during cold acclimation, deacclimation and reacclimation of winter wheat. - Plant Physiol. Biochem. 46: 970-976, 2008. Go to original source...
    39. Vítámvás, P., Saalbach, G., Prášil, I.T., Čapková, V., Opatrná, J., Jahoor, A.: WCS120 protein family and proteins soluble upon boiling in cold-acclimated winter wheat. - J. Plant Physiol. 164: 1197-1207, 2007. Go to original source...
    40. Walker, D.J., Romero, P., Correal, P.: Cold tolerance, water relations and accumulation of osmolytes in Bituminaria bituminosa. - Biol. Plant. 54: 293-298, 2010. Go to original source...
    41. Wolfraim, L.A., Langis, R., Tyson, H., Dhindsa, R.S.: cDNA sequence, expression, and transcript stability of a cold acclimation-specific gene, cas18, of alfalfa (Medicago falcata) cells. - Plant Physiol. 101: 1275-1282, 1993. Go to original source...
    42. Yao, K., Lockhart, K.M., Kalanack, J.J.: Cloning of dehydrin sequences from Brassica juncea and Brassica napus and their low temperature-inducible expression in germinating seeds. - Plant Physiol. Biochem. 43: 83-89, 2005. Go to original source...

    Return to the content