Biologia plantarum 54:141-144, 2010 | DOI: 10.1007/s10535-010-0022-2

Transformation of potato via Agrobacterium coated microparticle bombardment

T. T. Nguyen1,2, P. J. Dix1, G. D. Nugent1,3,*
1 Institute of Bioengineering and Agroecology, National University of Ireland, Maynooth, Ireland
2 Institute for Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam
3 School of Applied Sciences, Biotechnology and Environmental Biology, RMIT University, Victoria, Australia

The transformation of potato (Solanum tuberosum L. cv. Désirée) was extended by the Agrobacterium-mediated biolistic method. Using this approach transgenic shoots could be obtained at a similar frequency to that achieved through conventional biolistics. Leaves from shoot cultures were bombarded with gold particles coated in Agrobacterium tumefaciens cells harboring a binary plasmid encoding three genes of interest in the T-DNA. Nine shoots were obtained from 20 shots, with selection of transgenic shoots on a series of media containing progressively increasing concentrations of hygromycin from 5 to 20 mg dm-3.

Keywords: Agrobacterium transformation; ipt gene; Solanum tuberosum; transgenic potato
Subjects: Agrobacterium tumefaciens; β-glucuronidase; isopentenyltransferase; particle bombardment; polymerase chain reaction (PCR); potato; Solanum tuberosum; Southern blot analysis; transgenic plants

Received: April 15, 2008; Accepted: September 10, 2008; Published: March 1, 2010Show citation

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Nguyen, T.T., Dix, P.J., & Nugent, G.D. (2010). Transformation of potato via Agrobacterium coated microparticle bombardment. Biologia plantarum, 54(1), 141-144. doi: 10.1007/s10535-010-0022-2.
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