Biologia plantarum 54:177-180, 2010 | DOI: 10.1007/s10535-010-0030-2

Optimization of Algerian fir somatic embryos maturation

B. Vooková1,*, J. Machava2, A. Šalgovičová3, A. Kormuťák1
1 Institute of Plant Genetics and Biotechnology, SAS, Nitra, Slovak Republic
2 Department of Natural Environment, Faculty of Forestry, Zvolen, Slovak Republic
3 Institute of Forest Ecology, SAS, Nitra, Czechoslovakia

The effect of maturation pretreatment on development and growth of Abies numidica De Lann. somatic embryos was studied. The most beneficial was pre-culturing on Schenk and Hildebrandt medium without growth regulator for 2 weeks. Dry mass accumulation of emblings was lower than that of seedlings after 50 d of culturing. Contents of microelements in seedlings were higher than in emblings, but macroelements contents were higher in emblings. Contents of chlorophyll a and chlorophyll b in cotyledons were higher in seedlings than in emblings while no qualitative differences were detected between the protein profiles of seedlings and emblings.

Keywords: Abies numidica; chlorophyll; germination; macroelements; preculturing; proteins
Subjects: Abies numidica; Algerian fir; calcium; chlorophyll a,b; copper; in vitro culture, somatic embryogenesis; iron; magnesium; manganese; myo-inositol; phosphorus; potassium; sodium; zinc

Received: November 28, 2008; Accepted: July 23, 2009; Published: March 1, 2010Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Vooková, B., Machava, J., Šalgovičová, A., & Kormuťák, A. (2010). Optimization of Algerian fir somatic embryos maturation. Biologia plantarum, 54(1), 177-180. doi: 10.1007/s10535-010-0030-2.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Bertamini, M., Muthuchelian, K., Nedunchezhian, N.: Shade effect alters leaf pigments and photosynthetic responses in Norway spruce (Picea abies L.) grown under field conditions. - Photosynthetica 44: 227-234, 2006. Go to original source...
    2. Blödner, C., Majcherczyk, A., Kües, U., Polle, A.: Early drought-induced changes to the needle proteome of Norway spruce. - Tree Physiol. 27: 1423-1431, 2007. Go to original source...
    3. Fernández, M., Royo, M., Gil, L., Pardos, J.A.: Effects of temperature on growth and stress hardening development of phytotron-grown seedlings of Aleppo pine (Pinus halepensis Mill.). - Ann. forest. Sci. 60: 277-284, 2003.
    4. Fulgosi, H, Lepeduš, H., Cesar, V., Ljubešić, N.: Differential accumulation of plastid preprotein translocon components during spruce (Picea abies L. Karst.) needle development. - Biol. Chem. 368: 777-783, 2005. Go to original source...
    5. Hong, P.I., Chen, J.T., Chang, W.C.: Promotion of direct somatic embryogenesis of Onicidium by adjusting carbon sources. - Biol. Plant. 52: 597-600, 2008. Go to original source...
    6. Hu, Z., Hu, Y., Gao, H.H., Guan, X.Q., Zhuang, D.H.: Callus production, somatic embryogenesis and plant regeneration of Lycium barbarum root explants. - Biol. Plant. 52: 93-96, 2008. Go to original source...
    7. Jensen, U., Lixue, C.: Abies seed protein profile divergent from other Pinaceae. - Taxon 40: 435-440, 1991. Go to original source...
    8. Krajňáková, J., Gömöry, D., Häggman, H.: Somatic embryogenesis in Greek fir. - Can. J. Forest. Res. 38: 760-769, 2008. Go to original source...
    9. Laemmli, V.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. - Nature 227: 680-6851, 1970. Go to original source...
    10. Lichtenthaler, H.K.: Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. - Methods Enzymol. 148: 350-382, 1987. Go to original source...
    11. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobaco tissue cultures. - Physiol. Plant. 5: 473-497, 1962. Go to original source...
    12. Robakowski, P., Wyka, T., Samardakiewicz, S., Kierzkowski, D.: Growth, photosynthesis, and needle structure of silver fir (Abies alba Mill.) seedlings under different canopies. - Forest Ecol. Manage. 201: 211-227, 2004. Go to original source...
    13. Salajová, T., Salaj, J.: Somatic embryo formation on mature Abies alba × Abies cephalonica zygotic embryo explants. - Biol. Plant. 47: 7-11, 2003/04.
    14. Schenk, R.V., Hildebrandt, A.C.: Medium and techniques for induction and growth of monocotyledonous plant cell cultures. - Can. J. Bot. 50: 199-204, 1972. Go to original source...
    15. Schleppi, P., Tobler, L., Bucher, J.B., Wyttenbach, A.: Multivariate interpretation of the foliar chemical composition of Norway spruce (Picea abies). - Plant Soil. 219: 251-262, 2000. Go to original source...
    16. Szymura, T.H.: Differences in elemental composition of silver fir seedlings from four different populations in eastern Poland. - Forest Ecol. Manage. 179: 497-505, 2003. Go to original source...
    17. Šalgovičová, A., Kmet', J.: Influence of site conditions on physiological status of black walnut (Juglans nigra L.) stands. - Folia oecol. 31: 100-110, 2004.
    18. Von Aderkas, P., Kong, L., Hawkins, B., Rohr, R.: Efects of non-freezing low temperature on quality and cold tolerance of mature somatic embryos of interior spruce (Picea glauca (Moenoch) Voss × P. engelmannii Parri ex. Engelm.). - Propag. ornam. Plants 7: 112-121, 2007.
    19. Von Arnold, S., Sabala, I., Bozhkov, P., Dyachok, J., Filonova, L.: Developmental pathways of somatic embryogenesis. - Plant Cell Tissue Organ Cult. 69: 33-249, 2002. Go to original source...
    20. Vooková, B., Gajdošová, A., Matúšová, R.: Somatic embryogenesis in Abies alba × Abies alba and Abies alba × Abies nordmanniana hybrids. - Biol. Plant. 40: 523-530. 1997/98. Go to original source...
    21. Vooková, B., Kormut'ák, A.: Some futures of somatic embryo maturation of Algerian fir. - In Vitro cell. dev. Biol. Plant. 38: 549-551, 2002. Go to original source...
    22. Vooková, B., Kormut'ák, A., Hřib, J.: Effect of myo-inositol on somatic embryogenesis of Abies numidica. - J. appl. Bot. 75: 46-49, 2001.
    23. Wang, J., Sun, Y., Yan, S., Daud, M.K., Zhu, S.: High frequency plant regeneration from protoplasts in cotton via somatic embryogenesis. - Biol. Plant. 52: 616-620, 2008. Go to original source...
    24. Yang, X.M., An, L.Z., Xiong, Y.C., Zhang, Y.Li., Yu, S.J.: Somatic embryogenesis from immature zygotic embryos and monitoring the genetic fidelity of regenerated plants in grape vine. - Biol. Plant. 52: 209-214, 2008. Go to original source...

    Return to the content