Expression of Kanamycin resistance introduced byAgrobacteriutn binary vector intoNicotiana tabacum andAtropa belladonna

M. Ondrej, J. Vlasák

Biologia plantarum 1987, 29:161-166 | DOI: 10.1007/BF02876820

Kanamycin resistance gene was introduced into tobacco and Atropa belladonna cells by binary vectors, based on Agrobacterium, by means of inoculation of seedlings. The plasmid pGA472, which carries chimaeric kanamycin resistance gene expressed in plants was introduced by transformation into A. tumefaciens Bo542, harbouring pTiBo542 plasmid and A. rhizogenes 8196, carrying pRi8196 plasmids and the resulting two strains were used as binary vectors. Tobacco tumors induced by A. tumefaciens Bo542(pGA472) grew as undifferentiated, kanamycin resistant tissues. Those induced by A. rhizogenes 8196(pGA472) differentiated into transformed plants. When cultivated...

Book review

J. Neuzil

Biologia plantarum 1987, 29:166 | DOI: 10.1007/BF02876821

Activity of Mg²⁺-ATPase and cytochrome c-Oxidase in microsporocytes and anther wall during microsporogenesis inLarix europaea D.C.

Wiesława B. Chwirot, Alicja Górska-Brylass

Biologia plantarum 1987, 29:167-174 | DOI: 10.1007/BF02876822

Studies have been made on the activity of two mitochondrial enzymes, Mg²⁺ ATPase (E.C.3.6.1.3.) and cytochrome c-oxidase (E.C.I.9.3.2.) in microsporocytes and somatic cells of anther in larch.
The material for study were homogeneous fractions of microsporocytes from 15 stages of meiosis and the attendant anther somatic cells. The results have demonstrated that cells undergoing meiosis exhibit considerable mitochondrial metabolic activity. It is characterized by considerable variations in the activity level of both enzymes studied. The level and dynamics of variations of Mg²⁺-ATPase and cytochrome c-oxidase activity in microsporocytes...

Book review

Jana Pospíšilová

Biologia plantarum 1987, 29:174 | DOI: 10.1007/BF02876823

Anodic oxidation of coumarie and caffeic acids and their effects on nitrate uptake and nitrate reducta se inNicotia na tabacum cell suspension

F. Pospíšil, Marie Hrubcová, Milena Cvikková

Biologia plantarum 1987, 29:175-182 | DOI: 10.1007/BF02876824

Anodic oxidation of coumaric acid led to the inhibition of the process at the electrode due to a film which was formed after one-electron oxidation of the acid to phenoxy radical.By contrast, caffeic acid is oxidized in two steps-the phenoxy radical is formed in the first step, quinone in the second step. The inhibition of nitrate uptake by coumaric and caffeic acids is dependent on their concentration. 10^-4 M eaffeic acid totally inhibited nitrate uptake and the growth ofNicotiana tabacum cell suspension. 10^-6 M caffeic acid markedly inhibited nitrate uptake especially in the first three days after inoculation. 10^-6...

Book review

P. Žák

Biologia plantarum 1987, 29:182 | DOI: 10.1007/BF02876825

Histochemical study of vegetative and floral bud formation in tobacco stem expiants

Jana Opatrná, Tamara V. Bavrina, M. Kh. Chailakhyan

Biologia plantarum 1987, 29:183-188 | DOI: 10.1007/BF02876826

Primary explants from the inflorescence stem of tobacco and primary explants from the stem of vegetative plants, cultivatedin vitro under the same conditions, display different morphogenetic ability. The former give rise mostly to floral buds, whereas the latter exclusively to vegetative ones. Histological and histochemical analyses of both original andin vitro cultivated explants were made. They showed differences in chlorophyll content and alcohol dehydrogenase (AD) activity of the original explants reflecting their different metabolic status. Bud primordia were initiated in the superficial meristematic layer derived from epidermal...

Book review

Dagmar DykyjovÁ

Biologia plantarum 1987, 29:188 | DOI: 10.1007/BF02876827

Plant growth regulators and adventitious root development in relation to auxin

B. C. Jarvis, S. Yasmin

Biologia plantarum 1987, 29:189-198 | DOI: 10.1007/BF02876828

Adventitious root formation in stem cuttings of mung bean was enhanced by ethrel, which had an additive effect when employed simultaneously with indolebutyric acid (IBA). Abscisic acid (ABA) did not influence the number of roots per cutting whereas gibberellic acid (GA₃) and kinetin were without effect on rooting at lower concentrations but were inhibitory at higher concentrations. Nevertheless, all three of these chemicals showed synergistic interactions with IBA and/or indol-3-ylacetic acid (IAA) and thereby significantly promoted root formation. A localised application of morphactin to the epicotyl of cuttings totally inhibited root production...

The effect of methyl jasmonate on ethylene and l-aminocyclopropane-1-carboxylic acid production in apple fruits

M. Saniewski, J. Czapski, J. Nowacki, E. Lange

Biologia plantarum 1987, 29:199-203 | DOI: 10.1007/BF02876829

Methyl jasmonate (JA-Me) at concentration of 0.5 % and 1.0 % in lanolin paste applied to the surface of postclimacteric apples cultivars McIntosh, Spartan, and Cortland inhibited ethylene production in slices of cortex with a skin cut to a depth of about 2 mm. The level of 1-aminocyclopropane-l-carboxylic acid (ACC) was decreased in tissues of apples treated with methyl jasmonate. Methyl jasmonate stimulated ethylene production in preclimacteric apples cv. McIntosh.

Book review

J. Velemïnský

Biologia plantarum 1987, 29:203 | DOI: 10.1007/BF02876830

The function of metabolism in phosphorus accumulation in plant roots and its transport over long distances

I. Michalík

Biologia plantarum 1987, 29:204-213 | DOI: 10.1007/BF02876831

The values of influx (Ji) and efflux (Jo) of phosphates through intact maize roots (primary, seed roots) have confirmed the dependence of the P concentration in nutrient medium on the activity and efficiency of transport mechanism with respect to the accumulation of phosphates (J) by roots. The phosphate accumulation is about 97-99 % of the total uptake. If the P concentration is < 1 mM the efflux is negligible, and Ji <=g Jo. In contrast, if the P concentration is τ 1 mM, the proportion of efflux significantly increases, up to 45 % of the whole influx. The approximation to the conditions of equilibrium of phosphate flows ( Ji = Jo) depends...

Book reviews

P. Apel, J. Velemínský

Biologia plantarum 1987, 29:213 | DOI: 10.1007/BF02876832

Peptide hydrolases in senescing ragi leaves

K. B. Kumar, G. B. Mohanty, S. Patnaik, A. Bhuyan, P. A. Khan

Biologia plantarum 1987, 29:214-220 | DOI: 10.1007/BF02876833

Two peptide hydrolases - one active at pH 7.0 and the other at pH 3.4 (SH-dependent) were detected in ragi (Eleusine coracana Gaertn. cv. PR 202) leaves. The absolute activities of both acid and neutral peptide hydrolases were move or less stable in attached leaves and exhibited a mild rise in excised leaves, whereas the specific activities exhibited a significant rise. Light and growth regulators were ineffective in changing the course of absolute activity whereas cytokinins stimulated specific activities of both acid and neutral peptide hydrolases. Though the rates were different, protein decline was positively correlated with the changes...

Diurnal variations of potassium content in lucerne plants

F. Plhák

Biologia plantarum 1987, 29:221-229 | DOI: 10.1007/BF02876834

Diurnal changes in K content in leaf blades, petioles, stems and roots of eleven lucerne genotypes were followed. Significant positive correlations between changes in K content in petioles and upper half of stems and significant negative correlations between changes of K content in leaf blades and lower half of stems reflected rapid K movement. The velocity - up to 60 μmol g-1 (f.m.)h-1 - of changes in K content from leaf blades to lower part of stems and the other way round showed that long distance phloem transport occurred. Only moderate increase of K content contemporarily took place in roots. When total K amount in the whole plant was calculated...

Possible role of cytokinins in cereals with regard to the resistance to obligate fungus parasites

Gabriela VizÁrovÁ

Biologia plantarum 1987, 29:230-233 | DOI: 10.1007/BF02876835

The cultivars of barley and wheat resistant to mildew had a higher level of free zeatin and its derivatives during the whole ontogeny than the susceptible cultivars. The effect of exogenous application of cytokinins on the growth ofErysiphe graminis DC. was different. Kinetin and benzylaminopurine showed only a slight inhibitory effect. Zeatin and its derivatives completely inhibited growth of this fungus.

Book review

Z. Šesták, L. Nátr, V. Škrdleta, T. Gichner

Biologia plantarum 1987, 29:237-238 | DOI: 10.1007/BF02876837

Light gap detection by the photoblastic seeds ofCecropia obtusifolia andPiper auritum, two tropical rain forest trees

C. Vazquez-Yanes, A. Orozco-Segovia

Biologia plantarum 1987, 29:234-236 | DOI: 10.1007/BF02876836

The photoblastic seeds of two pioneer trees from the tropical rain forest-Cecropia obtusifolia andPiper auritum - require long diurnal periods of red light to germinate. In the field, the establishment of the trees takes place only in light gaps produced by falling trees. Experiments were performed in order to ascertain the relationship between the light requirements of the seeds and the germination control in the field. Results show that phytochrome regulated germination allows the detection of light gaps.

Dr. František Pospišil, DrSc. sexagenarian

Biologia plantarum 1987, 29:239-240 | DOI: 10.1007/BF02876838