Biologia plantarum 2018, 62:721-731 | DOI: 10.1007/s10535-018-0811-6

Mapping QTLs for chlorophyll content and chlorophyll fluorescence in wheat under heat stress

N. Bhusal1, P. Sharma2, S. Sareen2,*, A. K. Sarial3,4
1 Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar, Haryana, India
2 ICAR-Indian Institute of Wheat and Barley Research, Aggarsain Marg, Karnal, Haryana, India
3 Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Kaithal, Haryana, India
4 CSK Himachal Pradesh Krishi Vishva Vidyalaya, Palampur, Himachal Pradesh, India

Heat stress, one of the major abiotic stresses in wheat, affects chlorophyll fluorescence and chlorophyll content and thereby photosynthesis. To identify quantitative trait loci (QTLs) associated with these traits under terminal heat stress, 251 recombinant inbred lines (RILs) derived from a cross HD 2808/HUW510 were phenotyped. Using composite interval mapping, 40 QTLs were identified; 17 were related to conditions after timely sowing and 23 to heat stress after late sowing. The various parameters of chlorophyll fluorescence were associated with 23 QTLs, which were located on chromosomes 1A, 2A, 3A, and 2D and explained 3.67 to 18.04 % of phenotypic variation, whereas chlorophyll content was associated with 17 QTLs on chromosomes 2A, 2B, 2D, 5B, and 7A explaining 3.49 to 31.36 % of phenotypic variation. Most of the identified QTLs were clustered on chromosome 2D followed by 2A and 1A. The QTL Qchc.iiwbr-2A for chlorophyll content linked with marker gwm372 was stable over conditions and explained 3.81 to 18.05 % of phenotypic variation. In addition, 7 epistatic QTL pairs were also detected which explained 1.67 to 11.0 % of phenotypic variance. These identified genomic regions can be used in marker assisted breeding after validation for heat tolerance in wheat.

Keywords: composite interval mapping; phenotypic variation; RILs; timely and late sowing; Triticum aestivum
Subjects: QTL mapping; chlorophyll; chlorophyll fluorescence; RILs; linkage mapping; timely and late sowing; wheat
Species: Triticum aestivum

Received: September 27, 2017; Revised: March 23, 2018; Accepted: March 27, 2018; Published: August 1, 2018Show citation

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Bhusal, N., Sharma, P., Sareen, S., & Sarial, A.K. (2018). Mapping QTLs for chlorophyll content and chlorophyll fluorescence in wheat under heat stress. Biologia plantarum, 62(4), 721-731. doi: 10.1007/s10535-018-0811-6.
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