Biologia plantarum 55:469-478, 2011 | DOI: 10.1007/s10535-011-0112-9

Microsporogenesis and pollen formation in cassava

C. Wang1, Z. Lentini2, E. Tabares3, M. Quintero3, H. Ceballos3,4,*, B. Dedicova3, C. Sautter5, C. Olaya3, P. Zhang6
1 South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China
2 Universidad ICESI, Cali, Colombia
3 International Center for Tropical Agriculture, Cali, Colombia
4 Universidad Nacional de Colombia, Valle del Cauca, Colombia
5 Institute of Plant Sciences, Swiss Federal Institute of Technology, Zurich, Switzerland
6 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

This article describes the complete microsporogenesis and pollen formation in cassava (Manihot esculenta Crantz) at the various developmental stages (pollen mother cell, meiosis, tetrads, early free spore, mid uninucleate, late uninucleate, binucleate and mature pollen grain). Light microscopy, transmission electron microscopy and confocal laser scanning microscopy were used for the study. Floral bud size and other inflorescence characteristics were correlated with specific stages of the microspore development. This association allows a rapid selection of floral buds with similar microspore developmental stages, useful when a large number of homogeneous cells are needed for analysis and for in vitro induction of androgenesis. This article also compares methods for digestion the exine wall in microspores.

Keywords: CLSM; FISH; exine digestion; Manihot esculenta; ultrastructure

Received: December 10, 2009; Accepted: March 17, 2010; Published: September 1, 2011Show citation

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Wang, C., Lentini, Z., Tabares, E., Quintero, M., Ceballos, H., Dedicova, B., ... Zhang, P. (2011). Microsporogenesis and pollen formation in cassava. Biologia plantarum, 55(3), 469-478. doi: 10.1007/s10535-011-0112-9.
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