Biologia plantarum 55:479-484, 2011 | DOI: 10.1007/s10535-011-0113-8

Histomorphological changes in shoot apices of Lactuca sativa treated with gibberellic acid

O. Lee1,*, N. Sugiyama1
1 Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan

Lettuce plants were treated with gibberellic acid (GA3) and uniconazole (UZ; a gibberellin synthesis inhibitor) to investigate the influence of GA3 on cell division frequency in the shoot apical meristem (SAM) during stem elongation and flower initiation in lettuce (Lactuca sativa) grown in a greenhouse. GA3 (0.1 mM) was sprayed on the surface of outer leaves and uniconazole solution (0.86 mM) was applied to the soil. GA3 increased cell division frequency in the peripheral zone and the rib meristem of shoot apices, and this was associated with the stimulation of stem elongation. UZ treatment decreased cell division frequency in the peripheral zone, rib meristem and subapical pith, and this was associated with restricted stem elongation. Treatment with UZ and GA3 together induced minor stem elongation. Flower induction occurred 3 d earlier in the GA3 and UZ+GA3 treatments than in the control, while the UZ treatment delayed flower initiation for more than 9 d relative to the control.

Keywords: bolting; floral initiation; histone H4 gene; in situ hybridization; lettuce; stem elongation

Received: February 16, 2010; Accepted: October 11, 2010; Published: September 1, 2011Show citation

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Lee, O., & Sugiyama, N. (2011). Histomorphological changes in shoot apices of Lactuca sativa treated with gibberellic acid. Biologia plantarum, 55(3), 479-484. doi: 10.1007/s10535-011-0113-8.
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    References

    1. Bandstädter, J., Rossbach, C., Theres, K.: The pattern of histone H4 expression in the tomato shoot apex changes during development. - Planta 192: 69-74, 1994.
    2. Bernier, G.: Structural and metabolic changes in the shoot apex in transition to flowering. - Can. J. Bot. 49: 803-819, 1971. Go to original source...
    3. Chono, M., Suzuki, Y., Nemoto, K., Yamane, H., Murofushi, N., Yamaguchi, I.: Expression pattern of the CsPK3 auxin-responsive protein kinase gene. - Biosci. Biotechnol. Biochem. 65: 605-612, 2001. Go to original source...
    4. Chouard, P.: Vernalization and its relations to dormancy. - Annu. Rev. Plant Physiol. 11: 191-238, 1960. Go to original source...
    5. Fobert, P.R., Gaudin, V., Lunness, P., Coen, E.S., Doonana, J.H.: Distinct class of cdc2-related genes are differentially expressed during the cell division cycle in plants. - Plant Cell 8: 1465-1476, 1996. Go to original source...
    6. Gocal, G.F.W., Sheldon, C.C., Gubler, F., Moritz, T., Bagnall, D.J., MacMillan, C.P., Li, S.F., Parish, R.W., Dennis, E.S., Weigel, D., King, R.W.: GAMYB-like genes, flowering, and gibberellin signaling in Arabidopsis. - Plant Physiol. 127: 1682-1693, 2001. Go to original source...
    7. Hisamatsu, T., King, R.: The nature of floral signals in Arabidopsis. II. Roles for FLOWERING LOCUS T (FT) and gibberellins. - J. exp. Bot. 59: 3821-3829, 2008. Go to original source...
    8. Jacqmard, A.: Early effects of gibberellic acid on mitotic activity and DNA synthesis in the apical bud of Rudbeckia bicolor. - Physiol. vég. 6: 409-416, 1968.
    9. Jacqmard, A., Gadisseur, I., Bernier, G.: Cell division and morphological changes in the shoot apex of Arabidopsis thaliana during floral transition. - Ann. Bot. 91: 571-576, 2003. Go to original source...
    10. King, R.W., Moritz, T., Evans, L.T., Junttila, O., Herlt, A.J.: Long-day induction of flowering in Lolium temulentum involves sequential increases in specific gibberellins at the shoot apex. - Plant Physiol. 127: 624-632, 2001. Go to original source...
    11. King, R.W., Moritz, T., Evans, L.T., Martin, J., Andersen, C.H., Blundell, C., Kardailsky, I., Chandler, P.M.: Regulation of flowering in the long-day grass Lolium temulentum by gibberellins and the FLOWERING LOCUS T gene. - Plant Physiol. 141: 498-507, 2006. Go to original source...
    12. Kobayashi, M., Weigel, D.: Move on up, it's time for change: mobile signals controlling photoperiod-dependent flowering. - Genes Dev. 21: 2371-2384, 2007. Go to original source...
    13. Kouchi, H., Sekine, M., Hata, S.: Distinct classes of mitotic cyclins are differentially expressed in the soybean shoot apex during the cell cycle. - Plant Cell 7: 1143-1155, 1995. Go to original source...
    14. Lee, O., Nemoto, K., Sugiyama, N.: Histone H4 gene expression in shoot apices associated with floral initiation in lettuce. - J. jap. Soc. hort. Sci. 74: 121-126, 2005. Go to original source...
    15. Metzger, J.D., Hassebrock, A.T.: Selection and characterization of a gibberellin-deficient mutant of Thlaspi arvense (L.). - Plant Physiol. 94: 1655-1662, 1990. Go to original source...
    16. Mendu, M., Silflow, C.D.: Elevated levels of tubulin transcripts accompany the GA3-induced elongation of oat internode segment. - Plant Cell Physiol. 34: 973-983, 1993.
    17. Mutasa-Göttgens, E., Hedden, P.: Gibberellin as a factor in floral regulatory networks. - J. exp. Bot. 60: 1979-1989, 2009.
    18. Mutasa-Göttgens, E., Qi, A., Mathews, A., Thomas, S., Phillips, A., Hedden, P.: Modification of gibberellin signaling (metabolism and signal transduction) in sugar beet: analysis of potential targets for crop improvement. - Transgenic Res. 18: 301-308, 2009. Go to original source...
    19. Nishijima, T.: Gibberellin physiology and control of flowering and bolting of Japanese radish (Raphanus sativus L.). - Bull. nat. Res. Inst. Veg. Ornam. Plants Tea 15: 135-208, 2000.
    20. Peng, J., Harberd, N.P.: Gibberellin deficiency and response mutations suppress the stem elongation phenotype of phytochrome-deficient mutants of Arabidopsis. - Plant Physiol. 113: 1051-1058, 1997. Go to original source...
    21. Roux, F., Touzet, P., Cuguen, J., Le Corre, V.: How to be early flowering: an evolutionary perspective. - Trends Plant Sci. 11: 375-381, 2006. Go to original source...
    22. Sablowski, R.: Flowering and determinacy in Arabidopsis. - J. exp. Bot. 58: 899-907, 2007. Go to original source...
    23. Sachs, R.M.: Stem elongation. - Ann. Rev. Plant Physiol. 16: 73-96, 1965. Go to original source...
    24. Sachs, R.M., Bretz, R.F., Lang, A.: Shoot histogenesis: the early effects of gibberellins upon stem elongation in two rosette plants. - Amer. J. Bot. 46: 376-384, 1959. Go to original source...
    25. Sachs, R.M., Lang, A.: Effect of gibberellins on cell division in Hyoscyamus. - Science 125: 1144-1145, 1957. Go to original source...
    26. Saharan, V.: Effect of gibberellic acid combined with saponin on shoot elongation of Asparagus officinalis. - Biol. Plant. 54: 740-742, 2010. Go to original source...
    27. Sass, J.E.: Botanical Microtechnique. - The Iowa State University Press, Iowa 1958.
    28. Silverstone, A.L., Sun, T.: Gibberellins and the green revolution. - Trends Plant Sci. 5: 1-2, 2000. Go to original source...
    29. Steeves, T.A., Sussex, I.M.: Patterns in Plant Development. 2nd Ed. - Cambridge University Press, Cambridge 1989.
    30. Stuart, D.A., Durnam, D.J., Jones, R.L.: Cell elongation and cell division in elongating lettuce hypocotyls sections. - Planta 135: 249-255, 1977. Go to original source...
    31. Wilson, R.N., Heckman, J.W., Somerville, C.R.: Gibberellin is required for flowering in Arabidopsis thaliana under short days. - Plant Physiol. 100: 403-408, 1992. Go to original source...
    32. Yang, G., Jan, A., Komatsu, S.: Characterization of β-tubulin 4 regulated by gibberellins in rice leaf sheath. - Biol. Plant. 53: 422-428, 2009. Go to original source...
    33. Zanewich, K.P., Rood, S.B., Williams, P.H.: Growth and development of Brassica genotypes differing in endogenous gibberellin content. I. Leaf and reproductive development. - Physiol. Plant. 79: 673-678, 1990. Go to original source...

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