Biologia plantarum 55:647, 2011 | DOI: 10.1007/s10535-011-0163-y

Auxin is involved in the regulation of leaf and root development by LAF1 under short day conditions

B. S. Park1, W. G. Sang1, J. T. Song3, B. H. Lee4, J. H. Kim4, H. S. Seo1,2,*
1 Department of Plant Science, Seoul National University, Seoul, Korea
2 Korea Bio-MAX Institute, Seoul National University, Seoul, Korea
3 School of Applied Biosciences, Kyungpook National University, Daegu, Korea
4 Department of Biology, Kyungpook National University, Daegu, Korea

LAF1 (Long after far-red radiation 1) is a R2R3 Myb transcription factor and a signal transducer of far-red radiation. To investigate the role of LAF1 in leaf and root development, the leaf growth and vein patterning in laf1 mutants under short day conditions were examined. The length of rosette leaves was reduced and the width of the midvein was increased in laf1 mutants compared to their wild-type (WT) counterparts. In addition, cell size and cell number were both decreased in the laf1 mutant in comparison to the WT plant. A comparative analysis of gene expression showed that the transcript levels of PIN and IAA genes, encoding auxin carrier and response proteins, were decreased in laf1 mutants. LAF1 expression was also shown to be induced by 1-naphthaleneacetic acid. These results suggest that both auxin transport and auxin responses are impaired in laf1 mutants, and that LAF1 is involved in the regulation of leaf and root development mediated by auxin signaling under short day conditions.

Keywords: IAA; midvein; mutant; NAA; PIN; short day

Received: December 9, 2009; Accepted: May 13, 2010; Published: December 1, 2011Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Park, B.S., Sang, W.G., Song, J.T., Lee, B.H., Kim, J.H., & Seo, H.S. (2011). Auxin is involved in the regulation of leaf and root development by LAF1 under short day conditions. Biologia plantarum, 55(4), 647. doi: 10.1007/s10535-011-0163-y.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Ballesteros, M. L., Bolle, C., Lois, L. M., Moore, J. M., Vielle-Calzada, J. P., Grossniklaus, U., Chua, N. H.: LAF1, a MYB transcription activator for phytochrome A signaling. - Genes Dev. 15: 2613-2625, 2001. Go to original source...
    2. Barlier, I., Kowalczyk, M., Marchant, A., Ljung, K., Bhalerao. R., Bennett. M., Sandberg, G., Bellini, C.: The SUR2 gene of Arabidopsis thaliana encodes the cytochrome P450 CYP83B1, a modulator of auxin homeostasis. - Proc. nat. Acad. Sci. USA 97: 14819-14824, 2000. Go to original source...
    3. Benjamins, R., Scheres, B.: Auxin: the looping star in plant development. - Annu Rev Plant Biol. 59: 443-465, 2008. Go to original source...
    4. Canamero, R.C., Bakrim, N., Bouly, J.P., Garay, A., Dudkin, E.E., Habricot, Y., Ahmad, M.: Cryptochrome photoreceptors cry1 and cry2 antagonistically regulate primary root elongation in Arabidopsis thaliana. - Planta 224: 995-1003, 2006. Go to original source...
    5. Chen, M., Chory, J., Fankhauser, C.: Light signal transduction in higher plants. - Annu. Rev. Genet. 38: 87-117, 2004. Go to original source...
    6. Cheng, Y., Dai, X., Zhao, Y.: Auxin biosynthesis by the Yucca flavin monooxygenases controls the formation of floral organs and vascular tissues in Arabidopsis. - Genes Dev. 20: 1790-1799, 2006. Go to original source...
    7. Clack, T., Mathews, S., Sharrock, R.A.: The phytochrome apoprotein family in Arabidopsis is encoded by five genes: the sequences and expression of PHYD and PHYE. - Plant mol. Biol. 25: 413-427, 1994. Go to original source...
    8. Clough, S.J., Bent, A.F.: Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. - Plant J. 16: 735-743, 1998. Go to original source...
    9. Cluis, C.P., Mouchel, C.F., Hardtke, C.S.: The Arabidopsis transcription factor HY5 integrates light and hormone signalling pathways. - Plant J. 38: 332-347, 2004. Go to original source...
    10. De Smet, I., Jürgens, G.: Patterning the axis in plants - auxin in control. - Curr. Opin. Genet. Dev. 17: 337-343, 2007. Go to original source...
    11. Devlin, P.F., Yanovsky, M.J., Kay, S.A.: A genomic analysis of the shade avoidance response in Arabidopsis. - Plant Physiol. 133: 1617-1629, 2003. Go to original source...
    12. Ďurkovič, J., Bukovská, J.: Adventitious performance in micropropagated Cornus mas. - Biol. Plant. 53: 715-718, 2009.
    13. Haughn, G.W., Sommerville, C.: Sulfonylurea-resistant mutants of Arabidopsis thaliana. - Mol. gen. Genet. 204: 430-434, 1986. Go to original source...
    14. Hamann, T., Mayer, U., Jürgens, G.: The auxin-insensitive bodenlos mutation affects primary root formation and apicalbasal patterning in the Arabidopsis embryo. - Development 126: 1387-1395, 1999.
    15. Hobbie, L., McGovern, M., Hurwitz, L.R., Pierro, A., Liu, N.Y., Bandyopadhyay, A., Estelle, M.: The axr6 mutants of Arabidopsis thaliana define a gene involved in auxin response and early development. - Development 127: 23-32, 2000.
    16. Jang, I.-C., Yang, J.-Y., Seo, H.S., Chua, N.-H.: HFR1 is targeted by COP1 E3 ligase for post-translational proteolysis during phytochrome A signaling. Genes Dev. 19: 593-602, 2005. Go to original source...
    17. Jang, I.-C., Yang, S.W., Yang, J.Y., Chua, N.-H.: Independent and interdependent functions of LAF1 and HFR1 in phytochrome A signaling. - Genes Dev. 21: 2100-2111, 2007. Go to original source...
    18. Laxmi, A., Pan, J., Morsy, M., Chen, R.: Light plays an essential role in intracellular distribution of auxin efflux carrier PIN2 in Arabidopsis thaliana. - PloS One 3: e1510, 2008. Go to original source...
    19. Lin, R., Wang, H.: Two homologous ATP-binding cassette transporter proteins, AtMDR1 and AtPGP1, regulate Arabidopsis photomorphogenesis and root development by mediating polar auxin transport. - Plant Physiol. 138: 949-964, 2005. Go to original source...
    20. Mattsson, J., Sung, Z.R., Berleth T.: Responses of plant vascular systems to auxin transport inhibition. - Development 126: 2979-2991, 1999.
    21. Paponov, I.A., Teale, W.D., Trebar, M., Blilou, I., Palme, K.: The PIN auxin efflux facilitators: evolutionary and functional perspectives. - Trends Plant Sci. 10: 170-177, 2005. Go to original source...
    22. Peres, L.E.P., Zsögön, A., Kerbauy, G.B.: Abscisic acid and auxin accumulation in Catasetum fimbriatum roots growing in vitro with high sucrose and mannitol content. - Biol. Plant. 53: 560-564, 2009. Go to original source...
    23. Przemeck, G.K., Mattsson, J., Hardtke, C.S., Sung, Z.R., Berleth, T.: Studies on the role of the Arabidopsis gene MONOPTEROS in vascular development and plant cell axialization. - Planta 200: 229-237, 2006.
    24. Raven, J.A.: Transport of indoleacetic acid in plant cells in relation to pH and electrical potential gradients, and its significance for polar IAA transport. - New Phytol. 74: 163-172, 1975. Go to original source...
    25. Rubery, P.H., Sheldrake, A.R.: Carrier-mediated auxin transport. - Planta 118: 101-121, 1974. Go to original source...
    26. Salisbury, F.J., Hall, A., Grierson, C.S., Halliday, K.J.: Phytochrome coordinates Arabidopsis shoot and root development. - Plant J. 50: 429-438, 2007. Go to original source...
    27. Seo, H.S., Watanabe, E., Tokutomi, S., Nagatani, A., Chua, N.H.: Photoreceptor ubiquitination by COP1 E3 ligase desensitizes phytochrome A signaling. - Genes Dev. 18: 617-622, 2004. Go to original source...
    28. Seo, H.S., Yang, J.-Y., Ishikawa, M., Bolle, C., Ballesteros, M., Chua, N.-H.: LAF1 ubiquitination by COP1 controls photomorphogenesis and is stimulated by SPA1. - Nature 423: 995-999, 2003. Go to original source...
    29. Sharrock, R.A., Quail, P.H.: Novel phytochrome sequences in Arabidopsis thaliana: structure, evolution, and differential expression of a plant regulatory photoreceptor family. - Genes Dev. 3: 1745-1757, 1989. Go to original source...
    30. Sibout, R., Sukumar, P., Hettiarachchi, C., Holm, M., Muday, G.K., Hardtke, C.S.: Opposite root growth phenotypes of hy5 versus hy5 hyh mutants correlate with increased constitutive auxin signaling. - PLoS Genet. 2: e202, 2006. Go to original source...
    31. Sidler, M., Hassa, P., Hasan, S., Ringli, C., Dudler, R.: Involvement of an ABC transporter in a developmental pathway regulating hypocotyl cell elongation in the light. - Plant Cell 10: 1623-1636, 1998. Go to original source...
    32. Sieburth, L.E.: Auxin is required for leaf vein pattern in Arabidopsis. - Plant Physiol. 121: 1179-1190, 1999. Go to original source...
    33. Sieburth, L.E., Deyholos, M.K.: Vascular development: the long and winding road. - Curr. Opin. Plant Biol. 9: 48-54, 2006. Go to original source...
    34. Teale, W.D., Paponov, I.A., Palme, K.: Auxin in action: signalling, transport and the control of plant growth and development. - Nat. Rev. mol. Cell. Biol. 7: 847-859, 2006. Go to original source...
    35. Tian, Q., Uhlir, N.J., Reed, J.W.: Arabidopsis SHY2/IAA3 inhibits auxin-regulated gene expression. - Plant Cell 14: 301-319, 2002. Go to original source...
    36. Tian, Q., Nagpal, P., Reed, J.W.: Regulation of Arabidopsis SHY2/IAA3 protein turnover. - Plant J. 36: 643-651, 2003. Go to original source...
    37. Zhao, Y., Christensen, S.K., Fankhauserm, C., Cashman, J.R., Cohen, J.D., Weigel, D., Chory, J.: A role for flavin monooxygenase-like enzymes in auxin biosynthesis. - Science 291: 306-309, 2001. Go to original source...

    Return to the content