Biologia plantarum 45:581-584, 2002 | DOI: 10.1023/A:1022385327873

Micropropagation of Spilanthes acmella Murr.

K.V. Saritha1, E. Prakash2, N. Ramamurthy2, C.V. Naidu1
1 Department of Biotechnology, S.V. University, Tirupati, India
2 Biotechnology Research Centre, Tirupati, India

Multiple shoots of Spilanthes acmella Murr. were induced from hypocotyl segments obtained from 1-week-old seedlings on Murashige and Skoog's (MS) medium containing benzyladenine (BA), isopentenyl adenine, and naphthaleneacetic acid (NAA). High frequency shoot proliferation (95 %) and maximum number of shoots per explant (10 ± 0.6) were recorded with 0.5 mg dm-3 BA in combination with 0.1 mg dm-3 NAA. A proliferation was achieved by repeatedly subculturing the nodal segments on shoot multiplication medium. About 95 % of the in vitro shoots developed roots after transfer to half strength MS medium containing indole-3-butyric acid (1.0 mg dm-3). 95 % of the plantlets were successfully acclimatized and established in soil. Transplanted plantlets showed normal flowering without any morphological variation.

Keywords: direct regeneration; growth regulators; medicinal plant; tissue culture
Subjects: azocaseinase; cytokinins; in vitro culture, micropropagation; in vitro culture, nutrient media modification; Spilanthes acmella

Published: December 1, 2002Show citation

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Saritha, K.V., Prakash, E., Ramamurthy, N., & Naidu, C.V. (2002). Micropropagation of Spilanthes acmella Murr. Biologia plantarum, 45(4), 581-584. doi: 10.1023/A:1022385327873.
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