Biologia plantarum 46:635-636, 2003 | DOI: 10.1023/B:BIOP.0000041079.47888.ed

Molecular Cloning of a Phosphoenolpyruvate Carboxylase cDNA from Tropical Epiphytic CAM Orchid

C.R. Li1, X.B. Zhang2, C.S. Hew1
1 Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore
2 Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore

A full-length cDNA encoding phosphoenolpyruvate carboxylase (PEPC) was isolated from tropical epiphytic CAM orchid Mokara Yellow. The cDNA designated as Mpepc1 is 3 450 bp in length with an open reading frame of 2 862 bp encoding 954 amino acids. The deduced amino acid sequence of Mpepc1 shows 83 % identity with pepc2 of sorghum, 82 % with pepc1 and pepc2 of maize and 81 % with pepc of Arabidopsis thaliana. RT-PCR analysis showed that Mpepc1 was expressed in mature leaves, immature leaves, and aerial roots of M. Yellow. No expression was detected in the flower.

Keywords: amino acid sequence; Mokara Yellow; RACE
Subjects: amino acids; Arabidopsis thaliana; Arachnis hookeriana; Ascocenda sp.; Crassulacean acid metabolism; molecular cloning; orchids, phosphoenolpyruvate carboxylase; phosphoenolpyruvate carboxylase; reverse transcriptase polymerase chain reaction (RT-PCR)

Published: December 1, 2003Show citation

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Li, C.R., Zhang, X.B., & Hew, C.S. (2003). Molecular Cloning of a Phosphoenolpyruvate Carboxylase cDNA from Tropical Epiphytic CAM Orchid. Biologia plantarum, 46(4), 635-636. doi: 10.1023/B:BIOP.0000041079.47888.ed.
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