Biologia plantarum 48:179-184, 2004 | DOI: 10.1023/B:BIOP.0000033442.15611.7d

Agrobacterium-Mediated Transformation and Plant Regeneration of Triticum aestivum L.

N. Mitić1, R. Nikolić1, S. Ninković2, J. Miljuš-Djukić3, M. Nešković2
1 Center for Agricultural and Technological Research, Agriculture Research Institute "Serbia", Zaječar
2 Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade
3 Institute for Molecular Genetic and Genetic Engineering, Belgrade

The use of two Agrobacterium tumefaciens strains for transformation of Triticum aestivum L. cv. Vesna was studied. Immature embryos, isolated 15 d after pollination, were co-cultivated with the super-binary LBA4404/pTOK233 and the binary AGL1/pDM805 vectors. While the transient GUS-intron expression was high (69.9 and 80.0 %), the number of plants regenerated on selective media containing hygromycin or phosphinotricin did not exceed 0.4 and 0.13 %, respectively. Nevertheless, the regenerated plants were fertile and produced seeds. The T0 plants, as well as the T1 seedlings, displayed the activity in the β-glucuronidase histochemical assay and a positive signal in PCR analysis for the presence of uidA gene sequences in their genomes. The data suggest that the transformation of wheat cv. Vesna with both Agrobacterium strains is feasible.

Keywords: bar gene; GUS expression; hpt gene; immature embryos; regeneration; transgenic plants
Subjects: Agrobacterium-mediated transformation; Agrobacterium tumefaciens; β-glucuronidase; hygromycin; immature embryos; in vitro culture, nutrient media modification; in vitro culture, regeneration, organogenesis; nutrient medium, Murashige and Skoog; phosphinotricin; polymerase chain reaction (PCR); transformation, Agrobacterium-mediated; transgenic plants

Published: June 1, 2004Show citation

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Mitić, N., Nikolić, R., Ninković, S., Miljuš-Djukić, J., & Nešković, M. (2004). Agrobacterium-Mediated Transformation and Plant Regeneration of Triticum aestivum L. Biologia plantarum, 48(2), 179-184. doi: 10.1023/B:BIOP.0000033442.15611.7d.
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