Biologia plantarum 48:193-198, 2004 | DOI: 10.1023/B:BIOP.0000033444.17603.f2

Sequence Comparison of Plant Ornithine Decarboxylases Reveals High Homology and Lack of Introns

J.F. Jiménez-Bremont1,*, Y.M. Camacho-Villasana2, J.L. Cabrera-Ponce2, A.P. Barba de la Rosa1, N. Ochoa-Alejo2
1 Departamento de Biología Molecular, Instituto Potosino de Investigación Cientifíca y Tecnológica, Tangamanga, San Luis Potosí, México
2 Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Guanajuato, México

We have designed and constructed four oligonucleotides corresponding to the most conserved regions of ornithine decarboxylases (ODC; EC 4.1.1.17) of plant origin. These oligonucleotides were used for the amplification of homologous fragments from several plants (Zea mays, Capsicum annuum, Sorghum bicolor, Phaseolus vulgaris, Carica papaya and Daucus carota). The amplified fragments were cloned and sequenced, revealing high homology to other ODCs. Peptide sequences coded by these fragments were compared by Clustal analyses. These analyses identified the location of the conserved sequences corresponding to the binding sites of substrate and cofactor. Data demonstrated that the plant ODCs fragments lacked intron sequences and were extremely homologous (over 80 %), constituting a compact group separated from other eukaryotic ODCs.

Keywords: Capsicum annuum; Carica papaya; Daucus carota; Phaseolus vulgaris; polyamines; Sorghum bicolor; Zea mays
Subjects: amino acids; arginine decarboxylase; Capsicum annuum; Carica papaya; Clustal analyses; Datura stramonium; Daucus carota; dendrogram, phylogenetic tree; Nicotiana glutinosa; oligonucleotides; ornithine decarboxylases; Phaseolus vulgaris; phylogenetic tree, dendrogram; polyamines; polymerase chain reaction (PCR); Sorghum bicolor; Zea mays

Published: June 1, 2004Show citation

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Jiménez-Bremont, J.F., Camacho-Villasana, Y.M., Cabrera-Ponce, J.L., de la Rosa, A.P.B., & Ochoa-Alejo, N. (2004). Sequence Comparison of Plant Ornithine Decarboxylases Reveals High Homology and Lack of Introns. Biologia plantarum, 48(2), 193-198. doi: 10.1023/B:BIOP.0000033444.17603.f2.
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