Biologia plantarum 50:15-20, 2006 | DOI: 10.1007/s10535-005-0069-7

Evaluation of heterologous promoters in transgenic Populus tremula × P. alba plants

C. Studart-Guimaraes1, C. Lacorte1, A. C. M. Brasileiro1,*
1 Embrapa Recursos Geneticos e Biotecnologia, Laboratorio de Transferencia de Genes, Parque Estacao Biologica (PqEB), Brasilia-DF, Brazil

The pattern and expression level of β-glucuronidase (gus) reporter gene regulated by six heterologous promoters were studied in transgenic Populus tremula × P. alba plants obtained by Agrobacterium-mediated transformation. Binary vector constructs used contained the following promoter sequences: the CaMV35S from cauliflower mosaic virus; its duplicated version fused to the enhancer sequence from alfalfa mosaic virus; CsVMV from cassava vein mosaic virus; ubiquitin 3 from Arabidopsis thaliana (UBQ3); S-adenosyl-L-methionine synthetase (Sam-s) from soybean; and the rolA from Agrobacterium rhizogenes. Histochemical staining of root, stem and leaf tissues showed phloem and xylem-specific gus expression under rolA promoter, and constitutive expression with the other putative constitutive promoters. Quantitative GUS expression of 10 - 15 independently transformed in vitro grown plants, containing each promoter, was determined by fluorimetric GUS assays. The UBQ3-gus fusion induced the highest average expression level, although an extensive variation in expression levels was observed between independent transgenic lines for all the constructs tested.

Keywords: GUS; poplar; transformation; transgene
Subjects: Agrobacterium rhizogenes; antioxidants, antioxidative enzymes; Arabidopsis thaliana; β-glucuronidase; in vitro culture, embryogenesis, somatic embryogenesis; poplar; Populus alba; Populus tremula; Southern blot analysis; transgenic plants (cf. also in vitro…)

Received: February 16, 2004; Accepted: October 5, 2004; Published: March 1, 2006Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Studart-Guimaraes, C., Lacorte, C., & Brasileiro, A.C.M. (2006). Evaluation of heterologous promoters in transgenic Populus tremula × P. alba plants. Biologia plantarum, 50(1), 15-20. doi: 10.1007/s10535-005-0069-7.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Atkinson, H.J., Urwin, P.E., McPherson, M.J.: Engineering Plants for Nematode Resistance.-Annu. Rev. Phytopathol. 41: 1-25, 2003. Go to original source...
    2. Bhattacharyya, S., Dey, N., Maiti, I.B.: Analysis of cis-sequence of subgenomic transcript promoter from the Figwort mosaic virus and comparison of promoter activity with the cauliflower mosaic virus promoters in monocot and dicot cells.-Virus Res. 90: 47-62, 2002. Go to original source...
    3. Brasileiro, A.C.M., Tourneur, C., Leple, J.C., Combes, V., Jouanin, L.: Expression of the mutant Arabidopsis thaliana acetolactate synthase gene confers chlorsulfuron resistance to transgenic poplar plants.-Transgenic Res. 1: 133-141, 1992. Go to original source...
    4. Chunlin, S., Zhen, Z., Guifang, X., Altosaar, I., Pingzhang, F.: Construction of plant vectors with high level expression of B.t. toxin gene and studies on their expression behavior in transgenic tobaccos.-Chin. J. Biotechnol. 15: 203-210, 1999.
    5. Confalonieri, M., Balestrazzi, A., Bisoffi, S., Cella, R.: Factors affecting Agrobacterium tumefaciens-mediated transformation in several black poplar clones.-Plant Cell Tissue Organ Cult. 43: 215-222, 1995.
    6. Curtis, I.S., Power, J.B., Hedden, P., Phillips, A., Lowe, K.C., Ward, D.A., Davey, M.R.: Transformation and characterization of transgenic plants of Solanum dulcamara L.-incidence of transgene silencing.-Ann. Bot. 86: 63-71, 2000. Go to original source...
    7. Datla, R.S.S., Bekkaoui, F., Hammerlindl, J.K., Pilate, G., Dunstan, D.I., Crosby, W.L.: Improved high-level constitutive foreign gene expression in plants using an AMV RNA4 untranslated leader sequence.-Plant Sci. 94: 139-149, 1993. Go to original source...
    8. Falco, S.C., Zhongsen, L.: S-adenosyl-L-methionine synthetase promoter and its use in expression of transgenic genes in plants.-Patent Number WO 0037662, 2000.
    9. Feuillet, C., Lauvergeat, V., Deswarte, C., Pilate, G., Boudet, A., Grima-Pettenati, J.: Tissue-and cell-specific expression of a cinnamyl alcohol dehydrogenase promoter in transgenic poplar plants.-Plant mol. Biol. 27: 651-667, 1995. Go to original source...
    10. Fillatti, J.A., Haissig, B., McCown, B., Comai, L., Reimenschneider, D.: Development of glyphosate-tolerant Populus plants through expression of a mutant aroA gene from Salmonella typhimurium.-In: Hanover, J.W., Keathley, D.E. (ed.): Genetic Manipulation of Woody Plants. Pp. 243-249. Plenum Press, New York-London 1988. Go to original source...
    11. Gittins, J.R., Pellny, T.K., Biricolti, S., Hiles E.R., Passey, A.J., James D.J.: Transgene expression in the vegetative tissues of apple driven by the vascular-specific rolC and CoYMV promoters.-Transgenic Res. 12: 391-402, 2003. Go to original source...
    12. Grima-Pettenati, J., Goffner, D.: Lignin genetic engineering revisited.-Plant Sci. 145: 51-65, 1999. Go to original source...
    13. Guivarc'h, A., Carneiro, M., Vilaine, F., Pautot, V., Chriqui, D.: Tissue-specific expression of the rolA gene mediates morphological changes in transgenic tobacco.-Plant mol. Biol. 30: 125-134, 1996. Go to original source...
    14. Han, K.H., Meilan, R., Ma, C., Strauss, S.H.: An Agrobacterium tumefaciens transformation protocol effective on a variety of cottonwood hybrids (genus Populus).-Plant Cell Rep. 19: 315-320, 2000. Go to original source...
    15. Holsters, M., de Waele, D., Depicker, A., Messens, E., van Montagu, M., Schell, J.: Transfection and transformation of Agrobacterium tumefaciens.-Mol. gen. Genet. 163: 181-187, 1978. Go to original source...
    16. Holtorf, S., Apel, K., Bohlmann, H.: Comparison of different constitutive and inducible promoters for the overexpression of transgenes in Arabidopsis thaliana.-Plant mol. Biol. 29: 637-646, 1995. Go to original source...
    17. Jefferson, R.A., Kavanagh, T.A., Bevan, M.W.: GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.-EMBO J. 6: 3901-3907, 1987. Go to original source...
    18. Kamo, K., Blowers, A., McElroy, D.: Effect of the cauliflower mosaic virus 35S, actin, and ubiquitin promoters on uidA expression from a bar-uidA fusion gene in transgenic Gladiolus plants.-In Vitro cell. dev. Biol. Plant 36: 13-20, 2000. Go to original source...
    19. Kooter, J.M., Matzke, M.A., Meyer, P.: Listening to the silent genes: transgene silencing, gene regulation and pathogen control.-Trends Plant Sci. 4: 340-347, 1999. Go to original source...
    20. Kumar, S., Fladung M.: Gene stability in transgenic aspen (Populus). II. Molecular characterization of variable expression of transgene in wild and hybrid aspen.-Planta 213: 731-740, 2001. Go to original source...
    21. Lacorte, C., Barros, D.: Expression of gus and gfp genes in garlic (Allium sativum L.) cells following particle bombardment transformation.-J. Plant Biotechnol. 2: 135-142, 2000.
    22. Leple, J.C., Brasileiro, A.C.M., Michel, M.F., Delmotte, F., Jouanin, L.: Transgenic poplars: expression of chimeric genes using four different constructs.-Plant Cell Rep. 11: 137-141, 1992. Go to original source...
    23. Lindroth, A.M., Gronroos, R., Clapham, D., Svensson, J., Von Arnold, S.: Ubiquitous and tissue-specific gus expression in transgenic roots conferred by six different promoters in one coniferous and three angiosperm species.-Plant Cell Rep. 18: 820-828, 1999. Go to original source...
    24. McCabe, E.E., Swain, W.F., Martinelli, B.J., Christou, P.: Stable transformation of soybean (Glycine max) by particle bombardment.-Bio/Technology 6: 923-926, 1988.
    25. Mijnsbrugge, K.V., Van Montagu, M., Inze, D., Boerjan, W.: Tissue-specific expression conferred by the S-adenosyl-L-methionine synthase promoter of Arabidopsis thaliana in transgenic poplar.-Plant Cell Physiol. 37: 1108-1115, 1996. Go to original source...
    26. Norris, S.R., Meyer, S.E., Callis, J.: The intron of Arabidopsis thaliana polyubiquitin genes is conserved in location and is a quantitative determinant of chimeric gene expression.-Plant mol. Biol. 21: 895-906, 1993. Go to original source...
    27. Peleman, J., Boerjan, W., Engler, G., Seurinck, J., Botterman, J., Alliotte, T., Van Montagu, M., Inze, D.: Strong cellular preference in the expression of a housekeeping gene of Arabidopsis thaliana S-adenosylmethionine synthetase.-Plant Cell 1: 81-93, 1989a. Go to original source...
    28. Peleman, J., Saito, K., Cottyn, B., Engler, G., Seirinck, J., Van Montagu, M., Inze, D.: Structure and expression of the S-adenosylmethionine synthetase gene family in Arabidopsis thaliana.-Gene 84: 359-369, 1989b. Go to original source...
    29. Rishi, A.S., Nelson, N.D., Goyal, A.: Genetic modification for improvement of Populus.-Physiol. mol. Biol. Plants 7: 7-21, 2001.
    30. Rogers, S.O., Bendich, A.J.: Extraction of DNA from plant tissue.-In: Gelvin, S.B., Schilperoort, R.A. (ed.): Plant Molecular Biology Manual. Pp. 1-10. Kluwer Academic Publishers, Dordrecht 1988. Go to original source...
    31. Tanaka, A., Mita, S., Ohta, S., Kyozuka, J., Shimamoto, K., Nakakura, K.: Enhancement of foreing gene expression by a dicot intron in rice but not in tobacco is correlated with an increased level of mRNA and an efficient splicing of the intron.-Nucl. Acids Res. 18: 6767-6770, 1990. Go to original source...
    32. Verdaguer, B., De Kochko, A., Beachy, R.N., Fauquet, C.: Isolation and expression in transgenic tobacco and rice plants, of the cassava vein mosaic virus (CVMV) promoter.-Plant mol. Biol. 31: 1129-1139, 1996. Go to original source...
    33. Yibrah, H.S., Gronroos, R., Lindroth, A., Franzen, H., Clapham, D., Von Arnold, S.: Agrobacterium rhizogenes-mediated induction of adventitious rooting from Pinus contorta hypocotyls and the effect of 5-azacytidine on transgene activity.-Transgenic Res. 5: 75-85, 1996. Go to original source...

    Return to the content