Biologia plantarum 50:496-500, 2006 | DOI: 10.1007/s10535-006-0078-1

Micropropagation of Coleus blumei from nodal segments and shoot tips

G. Rani1, D. Talwar1, A. Nagpal1,*, G. S. Virk1
1 Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, India

A rapid and highly-effective method for micropropagation from nodal segment and shoot tip explants was established for Coleus blumei Benth. Nodal segments and shoot tips were inoculated on MS medium containing 0.7 % agar, 3 % commercial sugar, and different combinations of 6-benzyladenine (BA) with indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or α-naphthaleneacetic acid (NAA). Hundred percent shoot induction from both explants was achieved on the medium containing BA (2 mg dm-3) and NAA (1 mg dm-3). Shoot tips were proved to be the better explant in comparison to nodal segments in having high rate of shoot induction and more number of shoots. The same media conditions were found suitable for shoot multiplication. Multiplied shoots rooted best on MS medium supplemented with IBA (2 mg dm-3). Micropropagated plants were successfully established in soil after hardening, with 100 % survival rate.

Keywords: agar concentration; carbon source; growth regulators; shoot differentiation; tissue culture
Subjects: auxins; Coleus blumei; in vitro culture, agar, alginate; in vitro culture, micropropagation, propagation; in vitro culture, regeneration, proliferation, differentiation; medicinal plants; nutrient medium, Murashige and Skoog

Received: January 25, 2005; Accepted: September 21, 2005; Published: December 1, 2006Show citation

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Rani, G., Talwar, D., Nagpal, A., & Virk, G.S. (2006). Micropropagation of Coleus blumei from nodal segments and shoot tips. Biologia plantarum, 50(4), 496-500. doi: 10.1007/s10535-006-0078-1.
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    References

    1. Anand, A., Rao, C.S., Balakrishna, P.: In vitro propagation of Syzygium travancoricum Gamble-an endangered tree species.-Plant Cell Tissue Organ Cult. 56: 59-63, 1999. Go to original source...
    2. Bains, P.S.: A low cost and simple medium for micropropagation of potato.-In Vitro 27: 45A, 1991.
    3. Bhattacharya, S., Bhattacharya, S.: Rapid multiplication of Jasminum officinale L. by in vitro culture of nodal explants.-Plant Cell Tissue Organ Cult. 51: 57-60, 1997. Go to original source...
    4. Bhattacharya, R., Bhattacharya, S.: In vitro multiplication of Coleus forskohlii Briq.: an approach towards shortening the protocol.-In Vitro cell. dev. Biol. Plant 37: 572-575, 2001. Go to original source...
    5. Bringmann, G., Rischer, H., Schlauer, J., Aké Assi, L.: In vitro propagation of Ancistrocladus abbreviatus Airy Shaw (Ancistrocladaceae).-Plant Cell Tissue Organ Cult. 57: 71-73, 1999. Go to original source...
    6. Echeverrigaray, S., Basso, R., Andrade, L.B.: Micropropagation of Lavandula dentata from axillary buds of field-grown adult plants.-Biol. Plant. 49: 439-442, 2005. Go to original source...
    7. George, P.S., Ravishankar, G.A.: In vitro multiplication of Vanilla planifolia using axillary bud explants.-Plant Cell Rep. 16: 490-494, 1997.
    8. Hiregoudar, L.V., Kumar, H.G.A., Murthy, H.N.: In vitro culture of Feronia limonia (L.) Swingle from hypocotyls and internodal explants.-Biol. Plant. 49: 41-45, 2005. Go to original source...
    9. Ibrahim, K.M., Collins, J.C., Collin, H.A.: Characterization of progeny of Coleus blumei following an in vitro selection for salt tolerance.-Plant Cell Tissue Organ Cult. 28: 139-145, 1992. Go to original source...
    10. Iriondo, J.M., De la Iglesia, M., Perez, C.: Micropropagation of Elaeagnus angustifolia from mature trees.-Tree Physiol. 15: 691-693, 1995. Go to original source...
    11. Kannan, R., Jasrai, Y.T.: Micropropagation of medicinal plant-Vitex negundo.-J. med. arom. Plant Sci. 20: 693-696, 1998.
    12. Manjula, S., Thomas, A., Daniel, B., Nair, G.M.: In vitro plant regeneration of Aristolochia indica through axillary shoot multiplication and organogenesis.-Plant Cell Tissue Organ Cult. 51: 145-148, 1997. Go to original source...
    13. Marcotrigiano, M., Boyle, T.H., Morgan, P.A., Ambach, K.L.: Leaf color variants from Coleus shoot cultures.-J. amer. Soc. hort. Sci. 115: 681-686, 1990. Go to original source...
    14. Martin, K.P.: Rapid in vitro multiplication and ex vitro rooting of Rotula aquatica Lour., a rare rhoeophytic woody medicinal plant.-Plant Cell. Rep. 21: 415-420, 2003. Go to original source...
    15. Meinhard, J., Petersen, M., Alfermann, A.W.: Rosmarinic acid in organ cultures of Coleus blumei.-Planta med. 59(Suppl.): 7, 1993.
    16. Meyers, L.S., Grossen, N.E.: Behavioral Research, Theory, Procedure and Design.-W.H. Freeman and Co., San Francisco 1974.
    17. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue culture.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    18. Nayak, S., Sen, S.: Regeneration of Asparagus robustus Hort.-J. Herbs Spices med. Plants 5: 43-50, 1998.
    19. Petersen, M., Szabo, E., Meinhard, J., Karwatzki, B., Gertlowski, C.: Biosynthesis and accumulation of rosmarinic acid in suspension cultures of Coleus blumei.-Plant Cell Tissue Organ Cult. 43: 89-92, 1995. Go to original source...
    20. Ponsamuel, J., Samson, N.P., Anderson, K.P.: In vitro clonal propagation of country potato-an underexploited tuber plant.-In Vitro 30: A71, 1994.
    21. Sen, J., Sharma, A.K.: In vitro propagation of Coleus forskohlii Briq. for forskolin synthesis.-Plant Cell Rep. 9: 696-698, 1991. Go to original source...
    22. Sharma, N., Chandel, K.P.S., Srivastava, V.K.: In vitro propagation of Coleus forskohlii Briq., a threatened medicinal plant.-Plant Cell Rep. 10: 67-70, 1991. Go to original source...
    23. Sharma, S.K., Ramamurthy, V.: Micropropagation of 4-year-old elite Eucalyptus tereticornis trees.-Plant Cell Rep. 19: 511-518, 2000. Go to original source...
    24. Smith, R.H., Murashige, T.: Primordial leaf and phytohormone effects on excised shoot apical meristems of Coleus blumei Benth.-Amer. J. Bot. 69: 1334-1339, 1982. Go to original source...
    25. Sreekumar, S., Seeni, S., Pushpangadan, P.: Micropropagation of Hemidesmus indicus for cultivation and production of 2-hydroxy-4-methoxybenzaldehyde.-Plant Cell Tissue Organ Cult. 62: 211-218, 2000. Go to original source...
    26. Zagrajski, N., Leljak-Levanić, D., Jelaska, S.: Organogenesis and callogenesis in nodal, internodal and leaf explants of Coleus blumei Benth.-Periodicum Biol. 99: 67-76, 1997.

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