Biologia plantarum 53:69-74, 2009 | DOI: 10.1007/s10535-009-0010-6

β-1,3-glucanase activity in the stigma of healthy petunia flowers

A. M. Wakelin1, D. W. M. Leung1,*
1 School of Biological Sciences, University of Canterbury, Christchurch, New Zealand

β-1,3-Glucanase activity was detected in extracts of different tissues of healthy mature petunia flowers except the filament. The stigma was studied further as it had the highest enzyme activity and there is a paucity of information on the occurrence of this enzyme in this tissue. Specific activity of the enzyme was found to increase within the stigmatic tissue from early development until just before anthesis. Following non-denaturing polyacrylamide gel electrophoresis at pH 8.8, extracts of dehiscent stigma seem to contain three acidic isoforms of β-1,3-glucanase. Crude extracts of stigma was passed through a pachyman affinity column. A fraction of affinity-purified active β-1,3-glucanase enzyme was found to have no antifungal activity against Trichoderma viride, Phloma clematidina and Cladosporium fulvum.

Keywords: PAGE; pathogenesis-related proteins; Petunia hybrida; Solanaceae.
Subjects: antifungal activity; β-1,3-glucanase; pathogenesis-related (PR) proteins; petunia; Petunia hybrida; stigma

Received: December 7, 2005; Accepted: August 25, 2006; Published: March 1, 2009Show citation

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Wakelin, A.M., & Leung, D.W.M. (2009). β-1,3-glucanase activity in the stigma of healthy petunia flowers. Biologia plantarum, 53(1), 69-74. doi: 10.1007/s10535-009-0010-6.
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