Biologia plantarum 2012, 56:409-414 | DOI: 10.1007/s10535-011-0233-1

Arabidopsis thaliana in vitro shoot regeneration is impaired by silencing of TIR1

M. Qiao1, Z. -J. Zhao1, F. -N. Xiang1,*
1 The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, School of Life Sciences, Shandong University, Jinan, Shandong, P.R. China

Arabidopsis shoots regenerate from root explants through a two-step process consisting of pre-incubation on an auxin-rich callus induction medium (CIM), followed by transfer to a cytokinin-rich shoot induction medium (SIM). The auxin receptor gene TIR1 was up-regulated when explants were transferred to SIM. The CIM pre-incubation is required for its up-regulation. The tir1-1, TIR1 knockdown mutant, reduced the efficiency of shoot regeneration in tissue culture, while its over-expression mutant significantly improved efficiency. TIR1 promoter::GUS fusion analysis demonstrated that TIR1 expression was in the shoot and the newly emerging leaves. After 10 d on SIM, several cytokinin related genes (CDKB1;1, CKS1, IPT4 and ARR15), which associate with shoot regeneration, were up-regulated in plants over-expressing TIR1 and some of these were down-regulated in the tir1-1 mutant. Thus, TIR1 appears to be involved in regulating shoot regeneration.

Keywords: auxin receptor gene; auxin-rich medium; cytokinin related genes; cytokinin-rich medium; root explants
Subjects: auxin receptor gene; auxin-rich medium; cytokinin related genes; cytokinin-rich medium; root explants; 2.4-dichlorophenoxyacetic acid; kinetin; indoleacetic acid; GUS expression; PCR; transgenic plants; in vitro culture
Species: Arabidopsis thaliana

Received: February 22, 2011; Accepted: July 14, 2011; Published: September 1, 2012Show citation

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Qiao, M., Zhao, Z.-J., & Xiang, F.-N. (2012). Arabidopsis thaliana in vitro shoot regeneration is impaired by silencing of TIR1. Biologia plantarum, 56(3), 409-414. doi: 10.1007/s10535-011-0233-1.
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