Biologia plantarum 2013, 57:325-331 | DOI: 10.1007/s10535-012-0271-3

Metabolism and aluminum accumulation in Plantago almogravensis and P. algarbiensis in response to low pH and aluminum stress

N. Martins1, S. Gonçalves1, A. Romano1,*
1 Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology (IBB/CGB), Faculty of Sciences and Technology, University of Algarve, Faro, Portugal

We investigated the impact of low pH and aluminum on the metabolism and capacity for Al accumulation in shoots of the plantain species Plantago algarbiensis and P. almogravensis. We found that increasing the concentration of Al in the medium increased accumulation of it in the shoots of both plants (although more in P. almogravensis than in P. algarbiensis). The presence of Al in the medium induced proline and saccharide synthesis in P. almogravensis without affecting lipid peroxidation, but increased proline synthesis and lipid peroxidation in P. algarbiensis without affecting the saccharide content. Lipid peroxidation in P. algarbiensis was also enhanced at pH 4.0. The activity of antioxidant enzymes was increased as a response to low pH and Al in both species. Our data indicate that both species can accumulate high levels of Al but they have different sensitivities to low pH and/or the presence of Al in the growth medium.

Keywords: antioxidant enzymes; lipid peroxidation; proline; saccharides; tolerance
Subjects: aluminum; low pH; antioxidant enzymes; lipid peroxidation; proline; saccharides; stress tolerance; malondialdehyde; proteins; catalase; ascorbate peroxidase; peroxidase; superoxide dismutase
Species: Plantago almogravensis; Plantago algarbiensis

Received: April 11, 2012; Accepted: July 3, 2012; Published: June 1, 2013Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Martins, N., Gonçalves, S., & Romano, A. (2013). Metabolism and aluminum accumulation in Plantago almogravensis and P. algarbiensis in response to low pH and aluminum stress. Biologia plantarum, 57(2), 325-331. doi: 10.1007/s10535-012-0271-3.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Aebi, H.E.: Catalase. - In: Bergmeyer, H.U. (ed.): Methods of Enzymatic Analysis. Pp. 273-286. Verlag Chemie, Weinhern 1983.
    2. Andersson, M.E., Brunet, J.: Sensitivity to H and Al ions limiting growth and distribution of the woodland grass Bromus benekennii. - Plant Soil 153: 243-254, 1993. Go to original source...
    3. Baker, A.J.M., Brooks, R.R.: Terrestrial higher plants which hyperaccumulate metallic elements - a review of their distribution, ecology and phytochemistry. - Biorecovery 1: 81-126, 1989.
    4. Barnabas, B., Kovacs, G., Hegedus, A., Erdei, S., Horvath, G.: Regeneration of doubled haploid plants from in vitro selected microspores to improve aluminium tolerance in wheat. - J. Plant Physiol. 156: 217-222, 2000. Go to original source...
    5. Basu, U., Basu, A., Taylor, G.J.: Differential exudation of polypeptides by roots of aluminum-resistant and aluminumsensitive cultivars of Triticum aestivum L. in response to aluminum stress. - Plant Physiol. 106: 151-158, 1994. Go to original source...
    6. Beauchamp, C.O., Fridovich, I.: Superoxide dismutase: improved assays and assays applicable to acrylamide gels. - Anal. Biochem. 44: 276-287, 1971. Go to original source...
    7. Bradford, M.: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-254, 1976. Go to original source...
    8. Branquinho, C., Serrano, H.C., Pinto, M.J., Martins-Loução, M.A.: Revisiting the plant hyperaccumulation criteria to rare plants and earth abundant elements. - Environ. Pollut. 146: 437-443, 2007. Go to original source...
    9. Buurman, P., Jongmans, A.G.: Podzolization - an additional paradigm. - Edafologia 9: 107-114, 2002.
    10. Delhaize, E., Ryan, P.R.: Aluminum toxicity and tolerance in plants. - Plant Physiol. 107: 315-321, 1995. Go to original source...
    11. Egley, G.H., Paul, R.N., Vaughn, K.C., Duke, S.O.: Role of peroxidase in the development of water impermeable seed coats in Sida spinosa L. - Planta 157: 224-232, 1983. Go to original source...
    12. Ezaki, B., Nagao, E., Yamamoto, Y., Nakashima, S., Enomoto, T.: Wild plants, Andropogon virginidus L. and Miscanthus sinensis Anders, are tolerant to multiple stresses including aluminium, heavy metals and oxidative stresses. - Plant Cell Rep. 27: 951-961, 2008. Go to original source...
    13. Foy, C.D.: Physiological effects of hydrogen, aluminium, nad manganese toxicities in acid soil. - In: Adams, F. (ed.): Soil Acidity and Liming. 2nd Ed. Pp. 57-97. ASA, CSSA nad SSSA, Madison 1984.
    14. Giannakoula, A., Moustakas, M., Syros, T., Yupsanis, T.: Aluminum stress induces up-regulation of an efficient antioxidant system in the Al-tolerant maize line but not in the Al-sensitive line. - Environ. exp. Bot. 67: 487-494, 2010. Go to original source...
    15. Gonçalves, S., Martins, N., Romano, A.: Micropropagation nad conservation of endangered species Plantago algarbiensis and P. almogravensis. - Biol. Plant. 53: 774-778, 2009. Go to original source...
    16. Hodges, D.M., Delong, J.M., Forney, C.F., Prange, R.K.: Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. - Planta 207: 604-611, 1999. Go to original source...
    17. Kerven, G.L., Edwards, D.G., Asher, C.J., Hallman, P.S., Kokot, S.: Aluminum determination in soil solution. II. Short-term colorimetric procedures for the measurement of inorganic monomeric aluminum in the presence of organic acid ligands. - Aust. J. Soil Res. 27: 91-102, 1989. Go to original source...
    18. Khan, A.A., McNeilly, T., Collins, C.: Accumulation of amino acids, proline, and carbohydrates in response to aluminum and manganese stress in maize. - J. Plant Nutr. 23: 1303-1314, 2000. Go to original source...
    19. Kinraide, T.B.: Aluminum enhancement of plant growth in acid rooting media. A case of reciprocal alleviation of toxicity by two toxic cations. - Physiol. Plant. 88: 619-625, 1993. Go to original source...
    20. Kochian, L.V., Hoekenga, O.A., Pineros, M.A.: How do crop plants tolerate acid soils? Mechanisms of aluminium tolerance and phosphorus efficiency. - Annu. Rev. Plant Biol. 55: 459-493, 2004. Go to original source...
    21. Liu, P., Xu, G.D., Jiang, X.M., Ying, X.F.: The effect of aluminum on germination of soybean seed. - Seed 1: 30-32, 2003.
    22. Liu, Q., Yang, J.L., He, Y.Y., Zheng, S.J.: Effect of aluminum on cell wall, plasma membrane, antioxidants and root elongation in triticale. - Biol. Plant. 52: 87-92, 2008. Go to original source...
    23. Magné, C., Larher, F.: High sugar content of extracts interferes with colorimetric determination of aminoacid and free proline. - Anal. Biochem. 200: 115-118, 1992. Go to original source...
    24. Martins, N., Gonçalves, S., Palma, T., Romano, A.: the influence of low pH on in vitro growth and biochemical parameters of Plantago almogravensis and P. algarbiensis. - Plant Cell Tissue Organ Cult. 107: 113-121, 2011. Go to original source...
    25. Mittova, V., Guy, M., Tal, M., Volokita, M.: Salinity upregulates the antioxidative system in root mitochondria nad peroxisomes of the wild salt-tolerant tomato species Lycopersicon pennellii. - J. exp. Bot. 55: 1105-1113, 2004. Go to original source...
    26. Murashige, T., Shoog, F.: A revised medium for rapid growth and bio-assays with tobacco tissue cultures. - Physiol. Plant. 15: 473-497, 1962. Go to original source...
    27. Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. - Plant Cell Physiol. 22: 867-880, 1981.
    28. Olivares, E., Peña, E., Marcan, E., Mostacero, J., Aguiar, G., Benítez, M., Rengifo, E.: Aluminum accumulation and its relationship with mineral plant nutrients in 12 pteridophytes from Venezuela. - Environ. exp. Bot. 65: 132-141, 2009. Go to original source...
    29. Shamsi, I.H., Wei, K., Zhang, G.P., Jilani, G.H., Hassan, M.J.: Interactive effects of cadmium and aluminum on growth and antioxidative enzymes in soybean. - Biol. Plant. - 52: 165-169, 2008. Go to original source...
    30. Smirnoff, N.: Plant resistance to environmental stress. - Curr. Opin. Biotechnol. 9: 214-219, 1998. Go to original source...
    31. Tabaldi, L.A., Cargnelutti, D., Castro, G.Y., Gonçalves, J.F., Rauber, R., Bisognin, D.A., Schetinger, M.R.C., Nicoloso, F.T.: Effect of aluminum on the in vitro activity of acid phosphatases of four potato clones grown in three growth systems. - Biol. Plant. 55: 178-182, 2011. Go to original source...
    32. Troll, W., Lindsley, J.: A photometric method for the determination of proline. - J. biol. Chem. 215: 655-660, 1955.
    33. Von Uexküll, H.R., Mutert, E.: Global extent, development nad economic impact of acid soils. - Plant Soil 171: 1-15, 1995. Go to original source...
    34. Wen, X.-P., Ban, Y., Inoue, H., Matsuda, N., Moriguchi, T.: Aluminum tolerance in a spermidine synthase-overexpressing transgenic European pear is correlated with the enhanced level of spermidine via alleviating oxidative status. - Environ. exp. Bot. 66: 471-478, 2009. Go to original source...
    35. Xu, M., You, J., Hou, N., Zhang, H., Chen, G., Yang, Z.: Mitochondrial enzymes and citrate transporter contribute to the aluminium-induced citrate secretion from soybean (Glycine max) root. - Funct. Plant Biol. 37: 478, 2010. Go to original source...
    36. Xu, F.J., Li, G., Jin, C.W., Liu, W.J., Zhang, S.S., Zhang, Y.S., Lin, X.Y.: Aluminum-induced changes in reactive oxygen species accumulation, lipid peroxidation and antioxidant capacity in wheat root tips. - Biol. Plant. 56: 89-96, 2012. Go to original source...
    37. Yadav, S.K., Mohanpuria, P.: Responses of Camellia sinensis cultivars to Cu and Al stress. - Biol. Plant. 53: 737-740, 2009. Go to original source...
    38. Yemm, E.W., Willis, A.J.: The estimation of carbohydrates in plant extracts by anthrone. - Biochem. J. 57: 508-514, 1954. Go to original source...

    Return to the content