Biologia plantarum 2014, 58:231-240 | DOI: 10.1007/s10535-013-0387-0

Expression pattern of AtABCC13/MRP11 reveals developmental, hormonal, and nutritional regulations

T. El Guizani1,2, N. Blanc1, S. Triki2, B. St-Pierre1, E. Ducos1,*
1 EA2106, Biomolécules et Biotechnologies Végétales, Université François Rabelais de Tours, Tours, France
2 Faculté des Sciences de Tunis, Laboratoire de Biochimie, Tunis, Tunisia

ATP binding cassette (ABC) proteins constitute one of the largest families of transporters. In Arabidopsis thaliana, over 100 genes encoding ABC transporters have been identified. Here, we described the expression pattern of AtABCC13/MRP11, a member of the multidrug resistance associated protein MRP/ABCC subfamily, previously uncharacterized. The histochemical analysis of transgenic Arabidopsis harboring AtABCC13 promoter-β-glucuronidase gene fusion shows that the AtABCC13 expression was specifically associated with vascular bundles. Moreover, AtABCC13 displayed a complex hormonal regulation. β-glucuronidase (GUS) fluorimetric assays revealed that the gene expression was induced by gibberellic acid and downregulated by naphthalene acetic acid, abscisic acid, and zeatin. Because AtABCC13 is also expressed during seed development and during germination, its expression was assessed upon exposure to various nutrients: nitrate, phosphate, and sucrose stimulated the AtABCC13 expression in seedlings, whereas their lack strongly reduced it.

Keywords: ABC transporters; abscisic acid; gibberellic acid; β-glucuronidase; minerals; naphthalene acetic acid; plant development; saccharose; vascular bundles; zeatin
Subjects: ABC transporters; abscisic acid; gibberellic acid; β-glucuronidase; auxin; vascular bundles; root development; flowers; cytokinin; seed development; transgenic plans
Species: Arabidopsis thaliana

Received: May 2, 2013; Revised: July 16, 2013; Accepted: July 19, 2013; Published: June 1, 2014Show citation

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Guizani, T., Blanc, N., Triki, S., St-Pierre, B., & Ducos, E. (2014). Expression pattern of AtABCC13/MRP11 reveals developmental, hormonal, and nutritional regulations. Biologia plantarum, 58(2), 231-240. doi: 10.1007/s10535-013-0387-0.
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