Biologia plantarum 2014, 58:311-318 | DOI: 10.1007/s10535-014-0395-8

Exogenous 24-epibrassinolide ameliorates high temperature-induced inhibition of growth and photosynthesis in Cucumis melo

Y. P. Zhang1,2, J. He3, S. J. Yang2, Y. Y. Chen1,2,*
1 Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, P.R. China
2 Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai, P.R. China
3 College of Biosciences and Biotechnology, Yangzhou University, Yangzhou, P.R. China

This study was carried out to better understand the role of 24-epibrassinolide (EBR) in thermotolerance of melon (Cucumis melo L.). The melon seedlings were pretreated with various concentrations of EBR (0, 0.05, 0.1, 0.5, 1.0, and 1.5 mg dm-3) as foliar spray and then exposed to a high temperature (HT) stress. Exogenous EBR (0.5-1.5 mg dm-3) alleviated HT-caused growth suppression. In parallel, 1.0 mg dm-3 EBR attenuated the decrease in chlorophyll content, net photosynthetic rate, stomatal conductance, maximum quantum efficiency of photosystem (PS) II, quantum yield of PS II, and photochemical quenching of chlorophyll a fluorescence in HT-stressed plants, and inhibited transpiration rate and non-photochemical quenching. Furthermore, exogenous EBR also significantly reduced the content of malondialdehyde (MDA) and increased the content of soluble proteins and free proline, and activities of antioxidant enzymes including superoxide dismutase, guaiacol peroxidase, catalase, and ascorbate peroxidase under the HT stress. The results show that protective effects of EBR against the HT stress in the melon seedlings were most likely mediated through the improvement of photosynthesis and the stimulation of antioxidant capacity.

Keywords: ascorbate peroxidase; brassinosteroids; catalase; chlorophyll; malondialdehyde; melon; net photosynthetic rate; peroxidase; photosystem II; proline; stomatal conductance; superoxide dismutase; transpiration rate
Subjects: brassinosteroids; temperature - high; ascorbate peroxidase; catalase; chlorophyll; malondialdehyde; photosynthetic rate; peroxidase; photosystem II; proline; stomatal conductance; transpiration rate; superoxide dismutase; melon
Species: Cucumis melo

Received: January 21, 2013; Revised: August 20, 2013; Accepted: September 2, 2013; Published: June 1, 2014Show citation

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Zhang, Y.P., He, J., Yang, S.J., & Chen, Y.Y. (2014). Exogenous 24-epibrassinolide ameliorates high temperature-induced inhibition of growth and photosynthesis in Cucumis melo. Biologia plantarum, 58(2), 311-318. doi: 10.1007/s10535-014-0395-8.
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    References

    1. Ábrahám, E., Rigó, G., Székely, G., Nagy, R., Koncz, C., Szabados, L.: Light-dependent induction of proline biosynthesis by abscisic acid and salt stress is inhibited by brassinosteroid in Arabidopsis. - Plant mol. Biol. 51: 363-372, 2003. Go to original source...
    2. Aebi, H.: Catalase. - Methods Enzymol. 105: 121-126, 1984. Go to original source...
    3. Ahammed, G.J., Zhou, Y.H., Xia, X.J., Mao, W.H., Shi, K., Yu, J.Q.: Brassinosteroid regulates secondary metabolism in tomato towards enhanced tolerance to phenanthrene. - Biol. Plant. 57: 154-158, 2013. Go to original source...
    4. Aebi, H.: Catalase in vitro. - Methods Enzymol. 105: 121-126, 1984. Go to original source...
    5. Ainsworth, E.A., Ort, D.R.: How do we improve crop production in a warming world? - Plant Physiol. 154: 526-530, 2010. Go to original source...
    6. Ali, B., Hasan, S., Hayat, S., Hayat, Q., Yadav, S., Fariduddin, Q., Ahmad, A.: A role for brassinosteroids in the amelioration of aluminium stress through antioxidant system in mung bean (Vigna radiata L. Wilczek). - Environ. exp. Bot. 62: 153-159, 2008. Go to original source...
    7. Ali, B., Hayat, S., Ahmad, A.: 8-Homobrassinolide ameliorates the saline stress in chickpea (Cicer arietinum). - Environ. exp. Bot. 59: 217-223, 2007. Go to original source...
    8. Asthir, B., Koundal, A., Bains, N.S.: Putrescine modulates antioxidant defense response in wheat under high temperature stress. - Biol. Plant. 56: 757-761, 2012. Go to original source...
    9. Bajguz, A., Hayat, S.: Effects of brassinosteroids on the plant responses to environmental stresses. - Plant Physiol. Biochem. 47: 1-8, 2009. Go to original source...
    10. Bates, L.S., Waldren, R.P., Teare, I.D.: Rapid determination of free proline for water-stress studies. - Plant Soil 39: 205-207, 1973. Go to original source...
    11. Beauchamp, C., Fridovich, I.: Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. - Anal Biochem. 44: 276-287, 1971. Go to original source...
    12. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal Biochem. 72: 248-254, 1976. Go to original source...
    13. Calatayud, A., Barreno, E.: Response to ozone in two lettuce varieties on chlorophyll a fluorescence, photosynthetic pigments and lipid peroxidation. - Plant Physiol. Biochem. 42: 549-555, 2004. Go to original source...
    14. Dhaubhadel, S., Browning, K.S., Gallie, D.R., Krishna, P.: Brassinosteroid functions to protect the translational machinery and heat-shock protein synthesis following thermal stress. - Plant J. 29: 681-691, 2002. Go to original source...
    15. Ding, H.D., Zhu, X.H., Zhu, Z.W., Yang, S.J., Zha, D.S., Wu, X.X.: Amelioration of salt-induced oxidative stress in Solanum melongena L. by application of 24-epibrassinolide. - Biol. Plant. 56: 767-770, 2012. Go to original source...
    16. Dubey, R.S.: Photosynthesis in plants under stressful conditions. - In: Pessarakli, M. (ed.):Handbook of Photosynthesis. 2nd Ed. Pp. 717-737, CRC Press, Taylor and Francis Group, New York 2005.
    17. Fariduddin, Q., Yusuf, M., Hayat, Q., Ahmad, A.: Effects of 28-homobrassinolide on antioxidant capacity and photosynthesis in Brassica juncea plants exposed to different levels of copper. - Environ. exp. Bot. 66: 418-424, 2009. Go to original source...
    18. Farooq, M., Basra, S.M.A., Wahid, A., Rehman, H.: Exogenously applied nitric oxide enhances the drought tolerance in fine grain aromatic rice (Oryza sativa L.). J. Agron. Crop Sci. 195: 254-261, 2009. Go to original source...
    19. Farquhar, G.D., Sharkey, T.D.: Stomatal conductance and photosynthesis. - Annu. Rev. Plant Physiol. 33: 317-345, 1982. Go to original source...
    20. Gilmore, A.M.: Mechanistic aspects of xanthophyll cycle dependent photo-protection in higher plant chloroplasts and leaves. - Physiol. Plant 99: 197-209, 1997. Go to original source...
    21. Goda, H., Shimada, Y., Asami, T., Fujioka, S., Yoshida, S.: Microarray analysis of brassinosteroid-regulated genes in Arabidopsis. - Plant Physiol. 130: 1319-1334, 2002. Go to original source...
    22. Hasan, S.A., Hayat, S., Ahmad, A.: Brassinosteroids protect photosynthetic machinery against the cadmium induced oxidative stress in two tomato cultivars. - Chemosphere 84: 1446-1451, 2011. Go to original source...
    23. Hasan, S.A., Hayat, S., Ali, B.B., Ahmad. A.: 28-Homobrassinolide protects chickpea (Cicer arietinum) from cadmium toxicity by stimulating antioxidants. - Environ. Polut. 151: 60-66, 2008. Go to original source...
    24. Hayat, S., Hasan, S., Yusuf, M., Hayat, Q., Ahmad, A.: Effect of 28-homobrassinolide on photosynthesis, fluorescence and antioxidant system in the presence or absence of salinity and temperature in Vigna radiata. - Environ. exp. Bot. 69: 105-112, 2010. Go to original source...
    25. Hayat, S., Yadav, S., Wani, A., Irfan, S.M., Ahmad, A.: Comparative effect of 28-homobrassinolide and 24-epibrassinolide on the growth, carbonic anhydrase activity and photosynthetic efficiency of Lycopersicon esculentum. - Photosynthetica 49: 397-404, 2011. Go to original source...
    26. Janeczko, A., Oklestkova, J., Pociecha, E., Koscielniak, J., Mirek, M.: Physiological effects and transport of 24-epibrassinolide in heat-stressed barley. - Acta. Physiol. Plant. 33: 1249-1259, 2011. Go to original source...
    27. Jiang, M.Y., Zhang, J.H.: Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. - Plant Cell Physiol. 42: 1265-1273, 2001. Go to original source...
    28. Kagale, S., Divi, U.K., Krochko, J.E., Séller, W.A., Krishna, P.: Brassinosteroid confers tolerance in Arabidopsis thaliana and Brassica napus to a range of abiotic stresses. - Planta 225: 353-364, 2007.
    29. Li, Y.H., Liu, Y.J., Xu, X.L., Jin, M., An, L.Z., Zhang, H.: Effect of 24-epibrassinolide on drought stress-induced changes in Chorispora bungeana. - Biol. Plant. 56: 192-196, 2012. Go to original source...
    30. Lichtenthaler, H.K.: Cholorophylls and carotenoids: pigments of photosynthetic biomembranes. - Methods Enzymol. 148: 350-382, 1987. Go to original source...
    31. Liu, Y., Zhao, Z., Si, J., Di, C., Han, J., An, L.: Brassinosteriods alleviate chilling induced oxidative damage by enhancing antioxidant defense system in suspension cultured cells of Chorispora bungeana. - Plant Growth. Regul. 59: 207-214, 2009. Go to original source...
    32. Mavi, K., Demir, I.: Controlled deterioration and accelerated ageing tests predict relative seedling emergence potential of melon seed lots. - HortScience 42: 1431-1435, 2007. Go to original source...
    33. Mazorra, L.M., Holton N., Bishop G.J., Núñez M.: Heat shock response in tomato brassinosteroid mutants indicates that thermotolerance is independent of brassinosteroid homeostasis. - Plant Physiol. Biochem. 49: 1420-1428, 2011. Go to original source...
    34. Mittler, R.: Oxidative stress, antioxidants and stress tolerance. - Trends Plant Sci. 7: 405-410, 2002. Go to original source...
    35. Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. - Plant Cell Physiol. 22: 867-880, 1981.
    36. Nickel, R.S., Cunningham, B.A..: Improved peroxidase assay method using leuco-2,3',6-trichloroindophenol and application to comparative measurements of peroxidase catalysis. - Anal. Biochem. 27: 292-299, 1969. Go to original source...
    37. Ogweno, J.O., Son, X.S., Shi, K., Hu, W.H., Mao, W.H., Zhou, Y.H., Yu, J.Q., Nogués, S.: Brassinosteroid alleviate heatinduced inhibition of photosynthesis by increasing carboxylation efficiency and enhancing antioxidant systems in Lycopersicon esculentum. - J. Plant Growth Regul. 27: 49-57, 2008. Go to original source...
    38. Ramakrishna, B., Rao, S.S.R.: 24-Epibrassinolide alleviated zinc-induced oxidative stress in radish (Raphanus sativus L.) seedlings by enhancing antioxidative system. - Plant Growth Regul. 68: 249-259, 2012. Go to original source...
    39. Sasse, J.M.: Physiological actions of brassinosteroids: an update. - J. Plant Growth Regul. 22: 276-288, 2003. Go to original source...
    40. Saygideger, S., Deniz, F.: Effect of 24-epibrassinolide on biomass, growth and free proline concentration in Spirulina platensis(Cyanophyta)under NaCl stress. - Plant Growth Regul. 56: 219-223, 2008. Go to original source...
    41. Shahid, M.A., Pervez, M.A., Balal, R.M., Mattson, N.S., Rashid, A., Ahmad, R., Ayyub, C.M., Abbas, T.: Brassinosteroid (24-epibrassinolide) enhances growth and alleviates the deleterious effects induced by salt stress in pea (Pisum sativum L.). - Aust. J. Crop Sci. 5: 500-510, 2011.
    42. Singh, I., Shono, M.: Physiological and molecular effects of 24-epibrassinolide, a brassinosteroid, on thermotolerance of tomato. - Plant Growth Regul. 47: 111-119, 2005. Go to original source...
    43. Wahid, A., Gelani, S., Ashraf, M., Foolad, M.R.: Heat tolerance in plants: an overview. - Environ. exp. Bot. 61: 199-223, 2007. Go to original source...
    44. Wise, R.R., Olson, A.J., Schrader, S.M., Sharkey, T.D.: Electron transport is the functional limitation of photosynthesis in field-grown Pima cotton plants at high temperature. - Plant Cell Environ. 27: 717-724, 2004. Go to original source...
    45. Xia, X.J., Wang, Y.J., Zhou, Y.H., Tao, Y., Mao, W.H., Shi, K., Asami, T., Chen, Z., Yu, J.Q.: Reactive oxygen species are involved in brassinosteroids-induced stress tolerance in Cucumis sativus. - Plant Physiol. 150: 801-814, 2009. Go to original source...
    46. Yu, J.Q., Huang, L.F., Hu, W.H., Zhou, Y.H., Mao, W.H., Ye, S.F., Nogues, S.: A role for brassinosteroids in the regulation of photosynthesis in Cucumis sativus. - J. exp. Bot. 55: 1135-1143, 2004. Go to original source...
    47. Zhang, J., Li, D.M., Gao, Y., Yu, B., Xia, C.X., Bai, J.G.: Pretreatment with 5-aminolevulinic acid mitigates heat stress of cucumber. - Biol. Plant. 56: 780-784, 2012. Go to original source...

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