Biologia plantarum 2015, 59:65-73 | DOI: 10.1007/s10535-014-0462-1

Transcription of potassium transporter genes of KT/HAK/KUP family in peach seedlings and responses to abiotic stresses

Z.-Z. Song1,2, Y. Yang1, R.-J. Ma1, J.-L. Xu1, M.-L. Yu1,*
1 Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, and Jiangsu Key Laboratory of Horticultural Crop Genetic Improvement, Nanjing, P.R. China
2 State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, P.R. China

Potassium uptake and transport is facilitated by KT/HAK/KUP transporters. In this study, we identified 16 putative K+-uptake transporter genes in peach (Prunus persica). To investigate the role of PpeKUP in maintaing K+ uptake, transport, and homeostasis, we applied abiotic stresses to peach seedlings and analysed physiological reactions and transcriptional responses of PpeKUP genes. The peach seedlings were sensitive to polyethylene glycol (PEG), Pb, and Cd, as evidenced by impaired growth, K+ nutrition, and photosynthetic performance. However, the peach seedlings were tolerant to aluminum. K+ deficiency mainly increased, whereas K+ excess reduced the PpeKUP gene expression in roots. The Al treatments enhanced the PpeKUP transcription in shoots, whereas PEG, Pd, and Cd enhanced the PpeKUP transcription in all tissues. Our findings provided molecular basis for K+ uptake, transport, and homeostasis in the peach seedlings, and revealed potential candidate genes for further functional determination or breeding of peaches.

Keywords: aluminum; cadmium; gene expression; lead; osmotic stress; polyethylene glycol; Prunus persica
Subjects: KT/HAK/KUP; potassium transport; aluminum; cadmium; lead; osmotic stress; polyethylene glycol; gene expression; phylogenetic tree; net photosynthetic rate; stomatal conductance; chlorophyll; proline; peach
Species: Prunus persica

Received: April 3, 2014; Revised: May 21, 2014; Accepted: May 27, 2014; Published: January 1, 2015Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Song, Z.-Z., Yang, Y., Ma, R.-J., Xu, J.-L., & Yu, M.-L. (2015). Transcription of potassium transporter genes of KT/HAK/KUP family in peach seedlings and responses to abiotic stresses. Biologia plantarum, 59(1), 65-73. doi: 10.1007/s10535-014-0462-1.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    Supplementary files

    Download filebpl-201501-0008_S1.pdf

    File size: 119.6 kB

    References

    1. Achary, V.M., Parinandi, N.L., Panda, B.B.: Aluminum induces oxidative burst, cell wall NADH peroxidase activity, and DNA damage in root cells of Allium cepa L. - Environ. mol. Mutagen. 53: 550-60, 2012. Go to original source...
    2. Ashley, M.K., Grant, M., Grabov, A.: Plant responses to potassium deficiencies: a role for potassium transport proteins. - J. exp. Bot. 57: 425-436, 2006. Go to original source...
    3. Baňuelos, M.A., Garciadeblas, B., Cubero, B., Rodríguez-Navarro, A.: Inventory and functional characterization of the HAK potassium transporters of rice. - Plant Physiol. 130: 784-794, 2002. Go to original source...
    4. Basu, U., Godbold, D., Taylor, G.J.: Aluminum resistance in Triticum aestivum associated with enhanced exudation of malate. - J. Plant Physiol. 144: 747-753, 1994. Go to original source...
    5. Bose, J., Rodrigo-Moreno, A., Shabala, S.: ROS homeostasis in halophytes in the context of salinity stress tolerance. - J. exp. Bot. 65: DOI:10.1093/jxb/ert430, 2014. Go to original source...
    6. Choudhary, M., Jetley, U.K., Abash Khan, M., Zutshi, S., Fatma, T.: Effect of heavy metal stress on proline, malondialdehyde, and superoxide dismutase activity in the cyanobacterium Spirulina platensis-S5. - Ecotox. environ. Safety 66: 204-209, 2007. Go to original source...
    7. Davies, C., Shin, R., Liu, W., Thomas, M.R.: Transporters expressed during grape berry (Vitis vinifera L.) development are associated with an increase in berry size and berry potassium accumulation. - J. exp. Bot. 57: 3209-3216, 2006. Go to original source...
    8. Demiral, M.A., Köseoglu, A.T.: Effect of potassium on yield, fruit quality, and chemical composition of greenhouse-grown aalia melon. - J. Plant Nutr. 28: 93-100, 2005. Go to original source...
    9. Fu, H.H., Luan, S.: AtKuP1: a dual-affinity K+ transporter from Arabidopsis. - Plant Cell 10: 63-73, 1998.
    10. Grabov, A.: Plant KT/KUP/HAK potassium transporters: single family - multiple functions. - Ann. Bot. 99: 1035-1041, 2007. Go to original source...
    11. Gupta, M., Qiu, X., Wang, L., Xie, W., Zhang, C.J., Xiong, L.Z., Lian, X.M., Zhang, Q.F.: KT/HAK/KUP potassium transporters gene family and their whole-life cycle expression profile in rice (Oryza sativa). - Mol. Genet. Genomics 280: 437-452, 2008. Go to original source...
    12. Hartz, T.K., Johnstone, P.R., Francis, D.M., Miyao, E.M.: Processing tomato yield and fruit quality improved with potassium fertigation. - HortScience 40: 1862-1867, 2005.
    13. Jung, S., Staton, M., Lee, T., Blenda, A., Svancara, R., Abbott, A., Main, D.: GDR (Genome Database for Rosaceae): integrated web-database for Rosaceae genomics and genetics data. - Nucl. Acids Res. 36: D1034-D1040, 2008.
    14. Kumar, N., Kumar, S., Vats, S.K., Ahuja, P.S.: Effect of altitude on the primary products of photosynthesis and the associated enzymes in barley and wheat. - Photosynth. Res. 88: 63-71, 2006. Go to original source...
    15. Layne, D.R., Bassi, D.: The Peach: Botany, Production and Uses. - CABI, London 2008.
    16. Leser, G.E., Jifon, J.L., Makus, D.J.: Impact of potassium nutrition on postharvest fruit quality: melon (Cucumis melo L) case study. - Plant Soil 335: 117-131, 2009.
    17. Li, M., Li, Y., Li, H., Wu, G.: Overexpression of AtNHX5 improves tolerance to both salt and drought stress in Broussonetia papyrifera (L.) Vent. - Tree Physiol. 31: 349-357, 2011. Go to original source...
    18. Lombardi, L., Sebastiani, L.: Copper toxicity in Prunus cerasifera: growth and antioxidant enzymes responses of in vitro grown plants. - Plant Sci. 168: 797-802, 2005. Go to original source...
    19. Mäser, P., Thomine, S., Schroeder, J.I., Ward, J.M., Hirschi, K., Sze, H., Talke, I.N., Amtmann, A., Maathuis, F.J., Sanders, D., Harper, J.F., Tchieu, J., Gribskov, M., Persans, M.W., Salt, D.E., Kim, S.A., Guerinot, M.L.: Phylogenetic relationships within cation transporter families of Arabidopsis. - Plant Physiol. 126: 1646-1667, 2011.
    20. Mian, A., Oomen, R.J., Isayenkov, S., Sentenac, H., Maathuis, F.J., Véry, A.A.: Over-expression of an Na+-and K+-permeable HKT transporter in barley improves salt tolerance. - Plant J. 68: 468-479, 2011. Go to original source...
    21. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. - Physiol. Plant 15: 473-497, 1962. Go to original source...
    22. Nava, G., Dechen, A.R., Nachtigall, R.G.: Nitrogen and potassium fertilization affect apple fruit quality in Southern Brazil. - Commun. Soil Sci. Plant. 39: 96-107, 2007.
    23. Nieves-Cordones, M., Martinez-Cordero, M.A., Martinez, V., Rubio, F.: An NH4 +-sensitive component dominates high-affinity K+ uptake in tomato plants. - Plant Sci. 172: 273-280, 2007. Go to original source...
    24. Nounjan, N., Nghia, P.T., Theerakulpisut, P.: Exogenous proline and trehalose promote recovery of rice seedlings from salt-stress and differentially modulate antioxidant enzymes and expression of related genes. - J. Plant Physiol. 169: 596-604, 2012. Go to original source...
    25. Ozturk, Z.N., Talame, V., Deyholos, M., Michalowski, C.B., Galbraith, D.W., Gozukirmizi, N., Tuberosa, R., Bohnert, H.J.: Monitoring large-scale changes in transcript abundance in drought- and salt-stressed barley. - Plant mol. Biol. 48: 551-573, 2002. Go to original source...
    26. Rai, R.K., Singh, P., Shrivastava, A.K., Suman, A.: Modulation of low-temperature-induced biochemical changes in bud and root band zones of sugar cane sets by potassium, zinc, and ethrel for improving sprouting. - J. Agr. Food Chem. 56: 11976-11982, 2008. Go to original source...
    27. Ramalho, J.C., Fortunato, A.S., Goulao, L.F., Lidon, F.C.: Cold-induced changes in mineral content in leaves of Coffea spp. Identification of descriptors for tolerance assessment. - Biol. Plant. 57: 495-506, 2013. Go to original source...
    28. Rigas, S., Debrosses, G., Haralampidis, K., Vicente-Agullo, F., Feldmann, K.A., Grabov, A., Dolan, L., Hatzopoulos, P.: Trh1 encodes a potassium transporter required for tip growth in Arabidopsis root hairs. - Plant Cell 13: 139-151, 2001. Go to original source...
    29. Rubio, F., Santa-Maria, G.E., Rodriguez-Navarro, A.: Cloning of Arabidopsis and barley cDNAs encoding HAK potassium transporters in root and shoot cells. - Physiol. Plant 109: 34-43, 2000. Go to original source...
    30. Song, Z.Z., Su, Y.H.: Distinctive potassium-accumulation capability of alligator weed (Alternanthera philoxeroides) links to high-affinity potassium transport facilitated by K+-uptake systems. - Weed Sci. 61: 77-84, 2013. Go to original source...
    31. Song, Z.Z., Yang, S.Y., Zhu, H., Jin, M., Su, Y.H.: Heterologous expression of an alligator weed high-affinity potassium transporter gene enhances salinity tolerance in Arabidopsis. - Amer. J. Bot. 101: 840-850, 2014a. Go to original source...
    32. Song, Z.Z., Yang, S.Y., Zuo, J., Su, Y.H.: Over-expression of ApKUP3 enhances potassium nutritional status and drought tolerance in transgenic rice. - Biol. Plant. 58: 649-658, 2014b. Go to original source...
    33. Upadhyay, A., Upadhyay, A.K., Bhirangi, R.A.: Expression of Na+/H+ antiporter gene in response to water and salinity stress in grapevine rootstocks. - Biol. Plant. 56: 762-766, 2012. Go to original source...
    34. Vallejo, A.J., Peralta, M.L., Santa-Maria, G.E.: Expression of potassium-transporter coding genes, and kinetics of rubidium uptake, along a longitudinal root axis. - Plant Cell Environ. 28: 850-862, 2005. Go to original source...
    35. Véry, A.A., Sentenac, H.: Molecular mechanisms and regulation of K+ transport in higher plants. - Annu. Rev. Plant Biol. 54: 575-603, 2003. Go to original source...
    36. Wang, Y.H., Garvin, D.F., Kochian, L.V.: Rapid induction of regulatory and transporter genes in response to phosphorus, potassium, and iron deficiencies in tomato roots. Evidence for cross talk and root/rhizosphere-mediated signals. - Plant Physiol. 130: 1361-1370, 2002. Go to original source...
    37. Worthington, V.: Nutritional quality of organic versus conventional fruits, vegetables, and grains. - J. Altern. Complem. Med. 7: 161-173, 2001. Go to original source...
    38. Yu, M.L., Ma, R.J., Du, P., Song, H.F., Shen, Z.J., Zhang, Y., Xu, J.L.: [Breeding report on mid-season peach cultivar Xiahui 6.] - J. Fruit Sci. 22: 298-299, 2005. [In Chinese]
    39. Yurtseven, E., Kesmez, G.D., Ünlükara, A.: The effects of water salinity and potassium levels on yield, fruit quality and water consumption of a native Central Anatolian tomato species (Lycopersicon esculantum). - Agr. Water Manage. 78: 128-135, 2005. Go to original source...
    40. Zhao, D., Oosterhuis, D.M., Bednarz, C.W.: Influence of potassium deficiency on photosynthesis, chlorophyll content, and chloroplast ultrastructure of cotton plants. - Photosynthetica 39: 103-109, 2001. Go to original source...

    Return to the content