Biologia plantarum 46:613-615, 2003 | DOI: 10.1023/B:BIOP.0000041073.13794.0c

Molecular Cloning of ADP-Glucose Pyrophosphorylase Large Subunit cDNA from Oncidium

C.R. Li1, X.B. Zhang1, C.S. Hew1
1 Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore, Singapore

A full-length cDNA for ADP-glucose pyrophosphorylase large subunit (AGPL) was isolated from tropical epiphytic orchid Oncidium hybrid Goldiana. The cDNA was 1754 bp in length with an open reading frame of 1551 bp encoding 517 amino acids. The deduced amino acid sequence showed 73 % identity with those of potato isoform 3 (AGPL3) and Arabidopsis thaliana isoform 1 (AGPL1), 71 % identity with that of barley isoform BLPL. RT-PCR analysis showed that AGPL was expressed in mature leaf, immature leaf, developing inflorescence and flower of Oncidium. No expression was detected in roots.

Keywords: barley isoform BLPL; hybrid Goldiana; potato isoform AGPL3; RACE
Subjects: ADP-glucose pyrophosphorylase; amino acids; Arabidopsis thaliana; glucose biosynthesis; molecular cloning; Oncidium sp.; reverse transcriptase polymerase chain reaction (RT-PCR); saccharide biosynthesis

Published: December 1, 2003Show citation

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Li, C.R., Zhang, X.B., & Hew, C.S. (2003). Molecular Cloning of ADP-Glucose Pyrophosphorylase Large Subunit cDNA from Oncidium. Biologia plantarum, 46(4), 613-615. doi: 10.1023/B:BIOP.0000041073.13794.0c.
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