Biologia plantarum 46:187-192, 2003 | DOI: 10.1023/A:1022886107591

Responses of Peanut Somatic Embryos to Thidiazuron

M.V. Joshi1, N.A. Sahasrabudhe2, S. Hazra1
1 Plant Tissue Culture Division, National Chemical Laboratory, Pune-, India
2 Biochemical Sciences Division, National Chemical Laboratory, Pune-, India

Induction of both somatic embryogenesis and organogenesis in presence of thidiazuron is reported in peanut tissues. However the histological evidence of thidiazuron induced somatic embryogenesis was unclear. Thidiazuron triggered multiple shoot differentiation in the plumule of the embryos. Keeping in view the ability of thidiazuron to induce both organogenesis and embryogenesis in peanut tissues, experiments were conducted to define the pathway of morphogenesis in the plumule of rooted somatic embryos. On exposure to thidiazuron, projections appeared from the plumule. These projections closely resemble the somatic embryos. However histological examination revealed that these are caulogenic buds and not somatic embryos. In concurrence with the earlier reports on thidiazuron induced organogenesis it is concluded that this growth regulator induces organogenic response in peanut tissues.

Keywords: Arachis hypogaea; caulogenesis; organogenesis
Subjects: Arachis hypogaea; caulogenesis; cytokinins; in vitro culture, somatic embryogenesis; peanut, in vitro culture; thidiazuron

Prepublished online: March 1, 2003; Published: September 1, 2003Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Joshi, M.V., Sahasrabudhe, N.A., & Hazra, S. (2003). Responses of Peanut Somatic Embryos to Thidiazuron. Biologia plantarum, 46(2), 187-192. doi: 10.1023/A:1022886107591.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Akasaka, Y., Daimon, H., Mii, M.: Improved plant regeneration from cultured leaf segments in peanut (Arachis hypogaea L.) by limited exposure of thidiazuron.-Plant Sci. 156: 169-175, 2000. Go to original source...
    2. Ammirato, P.V.: Organizational events during somatic embryogenesis.-In: Green, C.D., Somer, D., Hackett, W.P., Biesboer, D.D. (ed.): Plant Tissue and Cell Culture. Pp. 57-81. Alan Liss, New York 1987.
    3. Baker, C.M., Wetzstein, H.Y.: Somatic embryogenesis and plant regeneration from leaflets of peanut (Arachis hypogaea).-Plant Cell Rep. 11: 71-75, 1992. Go to original source...
    4. Chengalrayan, K., Hazra, S., Gallo-Meagher, M.: Histological analysis of somatic embryogenesis and organogenesis induced from mature zygotic embryo-derived leaflets of peanut (Arachis hypogaea L).-Plant Sci. 161: 415-421, 2001. Go to original source...
    5. Chengalrayan, K., Mhaske, V.B., Hazra, S.: In vitro regulation of morphogenesis in peanut (Arachis hypogaea L.).-Plant Sci. 110: 259-268, 1995. Go to original source...
    6. Chengalrayan, K., Mhaske, V.B., Hazra, S.: High-frequency conversion of peanut somatic embryos.-Plant Cell Rep. 16: 783-786, 1997. Go to original source...
    7. Chengalrayan, K., Sathaye, S.S., Hazra, S.: Somatic embryogenesis from mature embryo-derived leaflets of peanut (Arachis hypogaea L.).-Plant Cell Rep. 13: 578-581, 1994. Go to original source...
    8. Durham, R., Parrot, W.: Repetitive somatic embryogenesis from peanut cultures in liquid medium.-Plant Cell Rep. 11: 122-125, 1992. Go to original source...
    9. Eapen, S., George, L., Rao, P.S.: Plant regeneration through somatic embryogenesis in peanut (Arachis hypogaea L.).-Biol. Plant. 35: 499-504, 1993. Go to original source...
    10. Gill, R., Saxena, P.K.: Direct somatic embryogenesis and regeneration of plants from seedling explants of peanut (Arachis hypogaea): promotive role of thidiazuron.-Can. J. Bot. 70: 1186-1192, 1992. Go to original source...
    11. Hazra, S., Sathaye, S.S., Mascarenhas, A.F.: Direct somatic embryogenesis in peanut (Arachis hypogaea).-Biotechnology 7: 949-951, 1989.
    12. Kanyand, M., Peterson, C.M., Prakash, C.S.: The differentiation of emergences into adventitious shoots in peanut, Arachis hypogaea (L.).-Plant Sci. 126: 87-95, 1997. Go to original source...
    13. Li, Z., Jarret, R.L., Pittman, R.N., Demski, J.M.: Shoot organogenesis from cultured seed explants of peanut (Arachis hypogaea L.) using thidiazuron.-In Vitro cell. dev. Biol. Plant 30: 187-191, 1994. Go to original source...
    14. Maheswaran, G., Williams, E.G.: Origin and development of somatic embryoids formed directly on immature embryos of Trifolium repens in vitro.-Ann. Bot. 56: 619-630, 1985. Go to original source...
    15. McKently, A.H.: Direct somatic embryogenesis from axes of mature peanut embryos.-In Vitro cell. dev. Biol. Plant 27: 197-200, 1991. Go to original source...
    16. McKently, A.H.: Effect of genotype on somatic embryogenesis from axes of mature peanut embryos.-Plant Cell Tissue Organ Cult. 42: 251-254, 1995. Go to original source...
    17. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    18. Murthy, B.N.S., Murch, S.J., Saxena, P.K.: Thidiazuron-induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea): endogenous growth regulator levels and significance of cotyledons.-Physiol. Plant 94: 268-276, 1995. Go to original source...
    19. Ozias-Akins, P.: Plant regeneration from immature embryos of peanut.-Plant Cell Rep. 8: 217-218, 1989. Go to original source...
    20. Ozias-Akins, P., Singsit, C., Branch, W.D.: Interspecific hybrid inviability in crosses of Arachis hypogaea × A. stenosperma can be overcome by in vitro embryo maturation or somatic embryogenesis.-J. Plant Physiol. 140: 207-212, 1992. Go to original source...
    21. Raghavan, V. (ed.): Experimental Embryogenesis in Vascular Plants.-Academic Press, London 1976.
    22. Raghavan, V. (ed.): Embryogenesis in Angiosperms: A Developmental and Experimental Study.-Cambridge University Press, New York 1986.
    23. Reddy, R.L., Reddy, G.M.: Factors affecting direct somatic embryogenesis and plant regeneration in groundnut, Arachis hypogaea L.-Indian J. exp. Biol. 31: 57-60, 1993.
    24. Sabitha Rani, A., Reddy, G.M.: Induction of somatic embryogenesis from young leaflets of cultivated and wild species of groundnut.-Indian J. exp. Biol. 34: 569-571, 1996.
    25. Sharma, A.K., Sharma, A.: Chromosome Techniques: Theory and Practice.-Butterworths, London 1980.
    26. Thakur, R.C., Hosoi, Y., Ishii, K.: Rapid in vitro propagation of Matteuccia struthiopteris (L.) Todaro-an edible fern.-Plant Cell Rep. 18: 203-208, 1998. Go to original source...
    27. Trigiano, R.N., Beaty, R.M., Graham, E.T.: Somatic embryogenesis from immature embryos of redbud (Cercis canadensis).-Plant Cell Rep. 7: 149-150, 1988. Go to original source...
    28. Venkatachalam, P., Geetha, N., Khandelwal, A., Shaila, M.S., Lakshmi Sita, G.: Induction of direct somatic embryogenesis and plant regeneration from cotyledon explants of Arachis hypogaea L.-Curr. Sci. 77: 269-273, 1999.
    29. Venkatachalam, P., Kavi Kishor, P.B., Jayabalan, N.: High frequency somatic embryogenesis and efficient plant regeneration from hypocotyl explants of groundnut.-Curr. Sci. 72: 271-275, 1997.
    30. Victor, J.M.R., Murch, S.J., Krishnaraj, S., Saxena, P.K.: Somatic embryogenesis and organogenesis in peanut: the role of thidiazuron and N6-benzylaminopurine in the induction of plant morphogenesis.-Plant Growth Regul. 28: 9-15, 1999b. Go to original source...
    31. Victor, J.M.R., Murthy, B.N.S., Murch, S.J., Krishnaraj, S., Saxena, P.K.: Role of endogenous purine metabolism in thidiazuron-induced somatic embryogenesis of peanut (Arachis hypogaea L.).-Plant Growth Regul. 28: 41-47, 1999a. Go to original source...
    32. Wetzstein, H.Y., Baker, C.M.: The relationship between somatic embryo morphology and conversion in peanut (Arachis hypogaea L.).-Plant Sci. 92: 81-89, 1993. Go to original source...

    Return to the content