Biologia plantarum 46:221-225, 2003 | DOI: 10.1023/B:BIOP.0000022255.01125.99

Functioning of the γ-Aminobutyrate Pathway in Wheat Seedlings Affected by Osmotic Stress

I. Bartyzel1, K. Pelczar1, A. Paszkowski1,*
1 Department of Biochemistry, Warsaw Agricultural University, Warszawa, Poland

γ-Aminobutyrate (GABA) was the only amino acid out of three amino acid intermediates of GABA shunt that increased significantly after 28 h from the beginning of osmotic stress induced by 20 % polyethylene glycol 6000 in wheat seedlings. At the same time specific activities of glutamate decarboxylase (GAD) and GABA aminotransferase (GABA-T) two enzymes of GABA pathway did not change as compared with the control plants. The response of two GABA-T activities (with pyruvate or 2-oxoglutarate as amino acid acceptor) to aminooxyacetate, 3-chloro-L-alanine and p-hydroxymercuribenzoate prompted us to suggest that at least two isoforms of GABA-T showing different substrate specificity do exist in wheat leaves.

Keywords: GABA aminotransferase; glutamate decarboxylase; GABA shunt; Triticum aestivum
Subjects: γ-aminobutyrate aminotransferase; amino acids; glutamate dehydrogenase; osmotic stress, γ-aminobutyrate pathway; polyethylene glycol, osmotic stress; Triticum aestivum; wheat, osmotic stress

Published: September 1, 2003Show citation

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Bartyzel, I., Pelczar, K., & Paszkowski, A. (2003). Functioning of the γ-Aminobutyrate Pathway in Wheat Seedlings Affected by Osmotic Stress. Biologia plantarum, 46(2), 221-225. doi: 10.1023/B:BIOP.0000022255.01125.99.
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