Biologia plantarum 46:277-280, 2003 | DOI: 10.1023/B:BIOP.0000022265.66891.73

Transgenic Rice Plants Expressing Bacillus subtilis Protoporphyrinogen Oxidase Gene Show Low Herbicide Oxyfluorfen Resistance

S.B. Ha1, S.B. Lee1, D.E Lee1, J.O. Guh2, K. Back1
1 Department of Genetic Engineering, Agricultural Plant Stress Research Center, Chonnam National University, Gwangju, South Korea
2 Division of Applied Plant Science, Chonnam National University, Gwangju, South Korea

Transgenic rice plants harbouring Bacillus subtilis protoporphyrinogen oxidase (Protox) gene, which is targeted into plastid, were generated by Agrobacterium-mediated transformation using a rice (Oryza sativa L. cv. Dongjin) and their gene integration at T1 generation by Southern and mRNA expression in T2 generation by Northern blotting were analyzed. Their herbicide-resistant trait was further confirmed by in vitro leaf segment assay and in planta bioassays such as seed germination assay and measurement of growth inhibition. The herbicide oxyfluorfen resistance in transgenic rice plants was not very high. The results showed that the Protox from B. subtilis can not be applicable as a gene source to generate a high level oxyfluorfen tolerance in plants.

Keywords: diphenylether herbicide; herbicide-resistant transgenic rice; leaf disc assay; Oryza sativa
Subjects: Agrobacterium-mediated tranformation; Agrobacterium tumefaciens; Bacillus subtilis; diphenylether herbicide; genetic transformation; herbicide resistance, oxyfluorfen; Oryza sativa; oxyfluorfen; protoporphyrinogen oxidase; resistance/tolerance to herbicides; rice, transgenic, protoporphyrinogen oxidase; transgenic plants

Published: September 1, 2003Show citation

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Ha, S.B., Lee, S.B., Lee, D.E., Guh, J.O., & Back, K. (2003). Transgenic Rice Plants Expressing Bacillus subtilis Protoporphyrinogen Oxidase Gene Show Low Herbicide Oxyfluorfen Resistance. Biologia plantarum, 46(2), 277-280. doi: 10.1023/B:BIOP.0000022265.66891.73.
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