Biologia plantarum 46:317-320, 2003 | DOI: 10.1023/A:1022835618494

Proline Accumulation Induced by Phosphinothricin in Rice Leaves

Y.-C. Tsai1, Y.T. Hsu1, C.H. Kao1
1 Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China

The effect phosphinothricin (PPT), an inhibitor of glutamine synthetase (GS), on proline accumulation in detached rice leaves was investigated. During 12 h incubation, PPT inhibited GS activity and induced accumulation of NH4 +, and accumulation of proline in the light but not in darkness. Proline accumulation caused by PPT in the light was related to protein hydrolysis, and increase in the contents of precursors of proline, ornithine and arginine. Abscisic acid accumulation was not required for proline accumulation in PPT-treated rice leaves.

Keywords: abscisic acid; arginine; glutamic acid; ornithine; ornithine-δ-aminotransferase; Oryza sativa; proline dehydrogenase; Δ1-pyroline-5-carboxylate reductase
Subjects: abscisic acid (ABA); amino acids; arginine; glutamic acid; glutamine synthetase; ornithine, ornithine-δ-aminotransferase; Oryza sativa; phosphinothricin, proline accumulation; proline accumulation, phosphinothricin; proline dehydrogenase; proteins, phosphinothricin; Δ1-pyroline-5-carboxylate reductase; rice, proline accumulation

Prepublished online: March 1, 2003; Published: September 1, 2003Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Tsai, Y.-C., Hsu, Y.T., & Kao, C.H. (2003). Proline Accumulation Induced by Phosphinothricin in Rice Leaves. Biologia plantarum, 46(2), 317-320. doi: 10.1023/A:1022835618494.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Aspinall, D., Paleg, L.G.: Proline accumulation: physiological aspects.-In: Paleg, L.G., Aspinall, D. (ed.): The Physiology and Biochemistry of Drought Resistance in Plants. Pp. 280-295. Academic Press, Sydney 1981.
    2. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    3. Brown, D.H., Fowden, L.: Metabolism of δ-acetylornithine in two leguminous species.-Phytochemistry 5: 887-892, 1966. Go to original source...
    4. Chen, C.T., Chen, L.M., Lin, C.C., Kao, C.H.: Regulation of proline accumulation in detached rice leaves exposed to excess copper.-Plant Soil 160: 283-290, 2001. Go to original source...
    5. Delauney, A.J., Verma, D.P.S.: Proline biosynthesis and osmoregulation in plants.-Plant J. 4: 215-223, 1993. Go to original source...
    6. Downs, C.G., Christey, M.C., Maddocks, D., Seelye, J.F., Stevenson, D.G.: Hairy roots of Brassica napus: I. Applied glutamine overcomes the effect of phosphinothricin treatment.-Plant Cell Rep. 14: 37-40, 1994. Go to original source...
    7. Fedina, I.S., Georgieva, K., Grigorova, I.: Light-dark changes in proline content of barley leaves under salt stress.-Biol. Plant. 45: 59-63, 2002. Go to original source...
    8. Hurst, P.L., King, G.A., Borst, W.M.: Postharvest inhibition of glutamine synthetase activity with phosphinothricin reduces the shelf-life of asparagus.-Postharvest Biol. Technol. 3: 327-334, 1993. Go to original source...
    9. Lea, P.J., Joy, K.W., Ramos, J.P., Guerrero, M.G.: The action of 2-amino-4-(methylphosphinyl)-butanoic acid (phosphinothricin) and its 2-oxo-derivative on the metabolism of cyanobacteria and higher plants.-Phytochemistry 23: 1-6, 1984. Go to original source...
    10. Leason, M.D., Cunliffe, D., Parkin, D., Lea, P.J., Miflin, B.J.: Inhibition of pea leaf glutamine synthetase by methionine sulfoximine, phosphinothricin and other glutamate analogue. - Phosphinothricin and other glutamate analogue.-Phytochemistry 21: 855-857, 1982. Go to original source...
    11. Lin, C.C., Kao, C.H.: Regulation of ammonium-induced proline accumulation in detached rice leaves.-Plant Growth Regul. 35: 69-74, 2001. Go to original source...
    12. Lin, J.-N., Kao, C.H.: Water stress, ammonium, and leaf senescence in detached rice leaves.-Plant Growth Regul. 26: 165-169, 1998. Go to original source...
    13. Lin, J.-N., Wang, J.-W., Kao, C.H.: Effect of abscisic acid and water stress on the senescence of detached rice leaves.-Biol. Plant. 42: 313-316, 1999. Go to original source...
    14. Lingnowski, E.M., Splittstoesser, W.E.: The change in arginine levels and metabolism of urea and ornithine in Cucurbita moschatta seedlings.-Physiol. Plant. 25: 225-229, 1971. Go to original source...
    15. McDonell, E.M., Coughlan, S.J., Wyn Jones, R.G.: Differential effects of abscisic acid on glycinebetaine and proline accumulation in three plant species.-Z. Pflanzenphysiol. 109: 207-213, 1983. Go to original source...
    16. Miflin, B.J., Lea, P.J.: The pathway of nitrogen assimilation in plants.-Phytochemistry 15: 873-885, 1976. Go to original source...
    17. Palmer, C.E., Oelck, M.: The relationship of phosphinothricin to growth and metabolism in cell cultures of Brassica napus L.-J. Plant Physiol. 141: 105-110, 1992.
    18. Perez-Garcia, A., de Vicente, A., Canton, F.R., Cazorla, F.M., Codina, J.C., Garcia-Gutierrez, A., Canovas, F.M.: Light-dependent changes of tomato glutamine synthetase in response to Pseudomonas syringae infection or phosphinothricin treatment.-Physiol. Plant. 102: 377-384, 1998. Go to original source...
    19. Sauer, H., Wild, A., Ruhle, W.: The effects of phosphinothricin (gluphosinate) on photosynthesis II. The causes of inhibition of photosynthesis.-Z. Naturforsch. 42c: 270-278, 1987. Go to original source...
    20. Tachibana, K., Watanabe, T., Seikizawa, Y., Takematsu, T.: Accumulation of ammonium in plants treated with bialaphos.-J. Pestic. Sci. 11: 33-37, 1986. Go to original source...
    21. Tsai, Y.-C., Chen, C.T., Hsu, Y.T., Kao, C.H.: The toxicity induced by phosphinothricin and methionine sulfoximine in rice leaves is mediated through ethylene but not abscisic acid.-Plant Growth Regul., in press, 2002. Go to original source...
    22. Tsai, Y.-C., Kao, C.H.: Light-dependent ammonium ion toxicity of rice leaves in response to phosphinothricin.-Biol. Plant. 45: 569-573, 2002. Go to original source...
    23. Wendler, C., Barniske, M., Wild, A.: Effect of phosphinothricin (glufosinate) on photosynthesis and photorespiration of C3 and C4 plants.-Photosynth. Res. 24: 55-61, 1990. Go to original source...
    24. Wendler, C., Wild, A.: Effect of phosphinothricin on photosynthesis and photorespiration.-Z. Naturforsch. 45c: 535-537, 1990. Go to original source...
    25. Wild, A., Sauer, H., Ruhle, W.: The effect of phosphinothricin (glufosinate) on photosynthesis. I. Inhibition of photosynthesis and accumulation of ammonia.-Z. Naturforsch. 42c: 263-269, 1987. Go to original source...
    26. Yang, C.W., Kao, C.H.: Ammonium in relation to proline accumulation in detached rice leaves.-Plant Growth Regul. 30: 139-144, 2000. Go to original source...
    27. Yoshiba, Y., Kiyosue, T., Nakashima, K., Yamagueni-Shinozaki, K., Shinozaki, K.: Regulation of levels of proline as an osmolyte in plants under water stress.-Plant Cell Physiol. 38: 1095-1107, 1997. Go to original source...

    Return to the content