Biologia plantarum 53:733-736, 2009 | DOI: 10.1007/s10535-009-0133-9

An efficient regeneration system of barley cultivars from leaf base segments

H. -P. Li1,2, T. Huang1, C. -X. Wang1, Y. -C. Liao1,3,*
1 Molecular Biotechnology Laboratory of Triticeae Crops, Huazhong Agricultural University, Wuhan, PR China
2 College of Life Science and Technology, Huazhong Agricultural University, Wuhan, PR China
3 College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, PR China

A simple and reliable regeneration system from leaf bases of barley (Hordeum vulgare L.) has been developed. The in vitro regeneration frequencies of seven commercial barley genotypes were compared using segments from the first leaves of 5-d-old seedlings. The regeneration frequency ranged from 31.56 to 72.22 % among the barley genotypes. Murashige and Skoog medium supplemented with 6-benzyladenine (0.5 mg dm-3) and kinetin (0.5 mg dm-3) was optimum for the regeneration. Longitudinal cut of the segments or the removal of coleoptiles further increased plantlet regeneration frequency.

Keywords: abscisic acid; benzyladenine; explants; Hordeum vulgare; kinetin
Subjects: auxins; barley; cytokinins; Hordeum vulgare; in vitro culture, indirect regeneration

Received: November 4, 2007; Accepted: April 25, 2008; Published: December 1, 2009Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Li, H.-P., Huang, T., Wang, C.-X., & Liao, Y.-C. (2009). An efficient regeneration system of barley cultivars from leaf base segments. Biologia plantarum, 53(4), 733-736. doi: 10.1007/s10535-009-0133-9.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Chung, H.H., Chen, J.T., Chang, W.C.: Plant regeneration through direct somatic embryogenesis from leaf explants of Dendrobium. - Biol. Plant. 51: 346-350, 2007. Go to original source...
    2. Dahleen, L.S., Bregitzer, P.: An improved media system for high regeneration rates from barley immature embryo-derived callus cultures of commercial cultivars. - Crop Sci. 42:934-938, 2002. Go to original source...
    3. Gless, C., Lörz, H., Jähne-Gärtner, A.: Transgenic oat plants obtained at high efficiency by microprojectile bombardment of leaf base segments. - J. Plant Physiol. 152: 151-157, 1998a. Go to original source...
    4. Gless, C., Lörz, H., Jähne-Gärtner, A.: Establishment of a highly efficient regeneration system from leaf base segments of oat (Avena sativa L.). - Plant Cell Rep. 17: 441-445, 1998b. Go to original source...
    5. Haliloglu, K.: Efficient regeneration system from wheat leaf base segments. - Biol. Plant. 50: 326-330, 2006. Go to original source...
    6. Maheswari, M., Lakshmi, J.N., Yadav, S.K., Varalaxmi, Y., Lakshmi, A.V., Vanaja, M., Venkateswarlu, B.: Efficient plant regeneration from shoot apices of sorghum. - Biol. Plant. 50: 741-744, 2006. Go to original source...
    7. Mingozzi, M., Morini, S.: In vitro cultivation of donor quince shoots affects subsequent morphogenesis in leaf explants. - Biol. Plant. 53: 141-144, 2009. Go to original source...
    8. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. - Physiol. Plant. 15: 473-497, 1962. Go to original source...
    9. Orban, N., Boldizsar, I., Boka, K.: Structural and chemical study of callus formation from leaves of Rubia tinctorum. - Biol. Plant. 51: 421-429, 2007. Go to original source...
    10. Pasternak, T.P., Rudas, V.A., Lörz, H., Kumlehn, J.: Embryogenic callus formation and plant regeneration from leaf base segments of barley (Hordeum vulgare L.). - J. Plant Physiol. 155: 371-375, 1999. Go to original source...
    11. Rengel, Z., Jelaska, S.: Somatic embryogenesis and plant regeneration from seedling tissues of Hordeum vulgare L. - Plant Physiol. 124: 385-392, 1986. Go to original source...
    12. Sujatha, M., Kumar, D.V.: In vitro bud regeneration of Carthamus tinctorius and wild Carthamus species from leaf explants and axillary buds. - Biol. Plant. 51: 782-786, 2007. Go to original source...
    13. Wang, C.T., Wei, Z.M.: Embryogenesis and regeneration of green plantlets from wheat (Triticum aestivum) leaf base. - Plant Cell Tissue Organ Cult. 77: 149-156, 2004. Go to original source...

    Return to the content