Intranet

Metabolomics

Metabolomics

The service department provides metabolomics analysis of biological materials (mainly blood serum and plasma) using liquid chromatography–mass spectrometry (LC–MS):

  • Combined untargeted and targeted analysis of complex lipids
  • Combined untargeted and targeted analysis of polar metabolites and exposome compounds
  • Targeted analysis of specific low-abundant lipids in cooperation with the Department of Adipose Tissue Biology

 

For combined untargeted and targeted methods liquid chromatography coupled with high-resolution mass spectrometry is used (Thermo Q Exactive Plus). Targeted analysis of specific lipids is performed using LC with a quadrupole/linear ion trap analyzer operated in multiple reaction monitoring (SCIEX QTRAP 5500).

 

The department processes metabolomic data using up-to-date software programs, including also identification of lipids, polar metabolites, and other detected compounds using MS/MS spectral library search. Merging untargeted and targeted workflows allows for high coverage of metabolites and, in many cases, their absolute quantification.

 

The data obtained are further processed using one-dimensional or multidimensional statistical methods.

Contacts

Institute of Physiology CAS
Department of Metabolomics

Videnska 1083
142 20 Prague 4
Czech Republic

tel.: +420 241 062 187

fax: +420 241 062 280

 
tomas.cajka@fgu.cas.cz

People

doc. Ing. Tomáš Čajka, Ph.D.
head of the department
 

Mgr. Petra Domašinská

research assistant

LC–MS, GLP

 

Ing. Veronika Palůchová

research assistant

LC–MS

 

Ing. Jiří Hricko

research assistant

LC–MS, GLP

 

Ing. Monika Černá

research assistant

LC–MS

 

Ing. Michaela Nováková

research assistant

statistics, bioinformatics