Biologia plantarum 2012, 56:502-508 | DOI: 10.1007/s10535-012-0050-1

cDNA-AFLP analysis reveals a set of new genes differentially expressed in cucumber root apexes in response to iron deficiency

G. Vigani1,*, A. Chittó1, P. De Nisi1, G. Zocchi1
1 Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Milano, Italy

Cucumber (Cucumis sativus L.) is considered a model plant for the investigation of Fe deficiency responses, since it strongly exhibits typical strategy I, i.e. increased activities of Fe(III)-chelate reductase, H+-ATPase and iron regulated transporters. In this study, cDNA amplified fragment length polymorphism analysis was employed to identify genes differentially expressed in the root apex following Fe deficiency. The expression patterns of the most interesting transcript derived fragments were validated by semiquantitative reverse trascriptase - polymerase chain reaction. A set of new genes overexpressed under Fe deficiency, such as those coding for calmodulin, SNAP, TIM23 and V-PPase were identified. Furthermore, we also observed that calmodulin protein accumulated in Fe-deficient root apexes.

Keywords: calmodulin; Cucumis sativus; semiquantitative RT-PCR; transcript-derived fragments; transcriptomic analysis
Subjects: calmodulin; transcriptomic analysis; PCR; AFLP; iron; transcript-derived fragments
Species: Cucumis sativus

Received: December 23, 2010; Accepted: June 22, 2011; Published: September 1, 2012Show citation

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Vigani, G., Chittó, A., Nisi, P., & Zocchi, G. (2012). cDNA-AFLP analysis reveals a set of new genes differentially expressed in cucumber root apexes in response to iron deficiency. Biologia plantarum, 56(3), 502-508. doi: 10.1007/s10535-012-0050-1.
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