Biologia plantarum - Latest articles
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Identification and characterization of catalase genes in Eleusine coracanaunder abiotic stressesOriginal Papers
S. SINGH, R. CHOPPERLA, S. KHAN, N. REDDY, J.C. PADARIA, A. MOLKUMAR, A.U. SOLANKE
Biologia Plantarum 63: 440-447, 2019 | DOI: 10.32615/bp.2019.048
Reactive oxygen species (ROS) are byproducts of metabolic processes such as respiration and photosynthesis in plants. Production of ROS leads to rapid cell damage, and plants developed a complex system of enzymatic and non-enzymatic antioxidants to scavenge these ROS. Catalase is an important enzyme, which plays a key role in elimination of toxic effects of hydrogen peroxide and plays a major role as an antioxidant. When characterizing heat responsive genes in finger millet (Eleusine coracana L.) using a suppression subtractive hybridization (SSH) library, we isolated two catalase genes and named them as EcCATA1 and EcCATB1....
Sense- and antisense-mediated resistance against Sri Lankan cassava mosaic virus (SLCMV) in Nicotiana benthamianaOriginal Papers
A. GOGOI, A. KALDIS, I. DASGUPTA, B.K. BORAH, A. VOLOUDAKIS
Biologia Plantarum 63: 455-464, 2019 | DOI: 10.32615/bp.2019.079
Sri Lankan cassava mosaic virus (SLCMV) is the principal causal agent of cassava mosaic disease in the Indian subcontinent. To gain resistance against the virus, the coat protein (CP) gene, namely the AV1 of SLCMV-Adivaram isolate, was cloned in either sense or antisense orientation under the Cauliflower mosaic virus 35S promoter, and transgenic Nicotiana benthamiana plants were obtained through Agrobacterium-mediated transformation. A total of eight T1 transgenic lines, four harboring the CP-sense construct and four harboring the CP-antisense construct were challenged with agro-infectious clones...
Multifunctional proline rich proteins and their role in regulating cellular proline content in plants under stressReviews
R.S. GUJJAR, A.D. PATHAK, S.G. KARKUTE, K. SUPAIBULWATANA
Biologia Plantarum 63: 448-454, 2019 | DOI: 10.32615/bp.2019.078
Proline rich proteins (PRPs), earlier famous as animal salivary proteins, have now been proven as indispensable plant proteins. They are highly rich in proline amino acid residues at the N-terminus whereas a characteristic eight cysteine motif is located at the C-terminus. The PRPs support a number of developmental processes from germination to plant death. Under normal environmental conditions, PRP genes express customarily in different plant parts depending on the specific function to be carried out. During abiotic stresses, PRP genes exhibit an uneven pattern of transcriptional regulation depending on the time and intensity of stress....
The intensity of and recovery from photoinhibition under drought in a thermotolerant common bean compared to drought tolerant genotypesOriginal Papers
D.C. MACEDO, G.R. LIMA, R.L.N. BARROS, C. PIMENTEL
Biologia Plantarum 63: 465-473, 2019 | DOI: 10.32615/bp.2019.076
The chlorophyll a fluorescence parameters of four Phaseolus vulgaris L. genotypes were evaluated under drought in two greenhouse experiments. Under severe water stress, the thermotolerant genotype 'Diplomata' maintained significantly higher values of predawn leaf water potential (Ψw), maximum Fv/Fm and effective (ΦPSII) quantum yield of photosystem II , and non-photochemical quenching than 'Ouro Negro', in the first experiment, and 'A 285' and 'A 222', in the second one. Among these parameters, Fv/Fm showed more differences that discriminated between the genotype...
Exogenous salicylic acid alleviates the oxidative damageof Arabidopsis thaliana by enhancing antioxidant defense systemsunder high lightOriginal Papers
Z.C. YANG, N. WU, L. TANG, X.H. YAN, M. YUAN, Z.W. ZHANG, S. YUAN, H.Y. ZHANG, Y.E. CHEN
Biologia Plantarum 63: 474-483, 2019 | DOI: 10.32615/bp.2019.074
To understand the protective roles of salicylic acid (SA) under high light, we investigated oxidative damage of Arabidopsis thaliana under high light in the presence or absence of SA. The results indicate that the high light led to an increase in the levels of proline, soluble sugars, reactive oxygen species, malondialdehyde, and electrolyte leakage, and a decrease in stomatal conductance (gs). Activities of six antioxidant enzymes increased significantly under the high light for 1 h. However, the high light for 3 h decreased the activities of peroxidase, superoxide dismutase, and catalase. In addition, we found that exogenous SA effectively...
Overexpression of the dominant negative nbexo70d1 mutantionconfers tolerance to salt stress in transgenic tobaccoOriginal Papers
N.N. TRINH, H.T. LE, T.P. NGUYEN
Biologia Plantarum 63: 484-495, 2019 | DOI: 10.32615/bp.2019.058
The vesicle trafficking process, which involves exocytotic and endocytotic pathways, has been reported to play a role in regulating plant responses to different environmental stresses. The Exo70 protein is important for the localization of the exocyst in the plasma membrane; however, its role in the physiology of stress tolerance is currently unclear. In this study, we characterized NbExo70D1, an Exo70 gene from tobacco (Nicotiana benthamiana). It was shown to have a role in the plant response to salt stress. More specifically, tolerance to salt stress is conferred by the overexpression of the dominant negative nbexo70d1 domain...
Influence of girdling on flower sex ratio, biochemical constituents, and fruit set intensity in mango (Mangifera indica L.)Original Papers
K. S. SHIVASHANKARA, G. A. GEETHA, and T. K. ROY
Biologia Plantarum 63: 432-439, 2019 | DOI: 10.32615/bp.2019.064
The study was conducted in mango to understand the relationship of various metabolites with flower sex ratio (hermaphrodite/male) and fruit set using stem girdling technique. Girdling one year old branches was carried out in two cultivars, Mangifera indica 'Alphonso' and 'Totapuri' to retain 50, 100 and 150 leaves to vary the content of hormones, sugars, total carbohydrates, and amino acids. The increased leaf number was found to increase the total carbohydrates, glucose, fructose, and other sugars. Girdling also significantly increased the concentration of abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), indole acetic acid,...
RNDr. Tomáš Gichner, DrSc.Obituary
Diana Stavreva
Biologia Plantarum 63: iv-v, 2019
A leading figure in the field of plant genetics
RNDr. Tomáš Gichner, DrSc.
passed away on 6th January 2019 at the age of 83.
Transcriptome profiling in Salix matsudana during refilling xylem vessels after embolismOriginal Papers
Y.C. Yu, J.X. Liu, Z.Y. Sun
Biologia Plantarum 63: 425-431, 2019 | DOI: 10.32615/bp.2019.061
The objective of this study is to understand the role of bark water uptake in refilling of xylem vessels after embolism in Salix matsudana. Further, we determined expression of genes closely related to xylem embolism repair. The isolated branch segments of S. matsudana were soaked in deionized water and after 2 h, the micro-computed tomography (CT) images, volume of xylem sap, osmotic potential of xylem sap, and the content of starch and soluble sugar were measured. At the same time, Illumina high-throughput sequencing (Hi Seq TM) was applied to obtain the xylem transcriptome profiling. The results showed that the soaked branches...
RNDr. Bohdan Slavík, DrSc.Obituary
J. Krekule
Biologia Plantarum 63: iii, 2019
A leading figure in the field of plant physiology
RNDr. Bohdan Slavík, DrSc.
passed away on 1st August 2018 at the age of 93.
Rosmarinic acid accumulation in Melissa officinalis shoot cultures is mediated by ABAOriginal Papers
S.-M. Mousavi, L. Shabani
Biologia Plantarum 63: 418-424, 2019 | DOI: 10.32615/bp.2019.057
Plant responses to elicitors are the result of a series of highly modulated consecutive changes in hormones or reactive oxygen species (ROS). Abscisic acid (ABA) is a stress hormone that coordinates the complex networks of stress responses and its content is rapidly changed in response to stresses. This study evaluated the effects of application of ABA (0, 5, 25, 50, and 100 µM) to shoot cultures of lemon balm (Melissa officinalis L.) in Murashige and Skoog (MS) liquid medium on growth, H2O2 production, rosmarinic acid (RA) content, total phenolic compound accumulation, phenylalanine-ammonia lyase (PAL)...
Growth, secondary metabolism, and related gene expression in response to interactions of nitrogen and sulfur in Isatis indigoticaOriginal Papers
Y.J. Miao, R.J. Qu, J.T. Sha, Y.W. Cao, J.L. Guan, J. Xu, X.Q. Tang, F.Q Wang, J. Yang
Biologia Plantarum 63: 411-417, 2019 | DOI: 10.32615/bp.2019.053
Nitrogen and sulfur are major elements influencing plant growth and production of secondary metabolites. They interact to each other, but little is known about it in Isatis indigotica Fort. plants. In this study, 15 different treatments representing all possible combinations of 3 N treatments (N1, N2, and N3, corresponding to 5, 15, and 25 mM N, respectively) and five S treatments (S0, S1, S2, S3, and S4, corresponding to 0.00, 1.25, 2.50, 5.00 and 7.50 mM S, respectively) were used, and plant growth, indigo and indirubin yields, and expressions of genes encoding enzymes involved in N and S metabolisms were measured. The results show that the...
Application of abscisic acid prevents leaf senescence at decreased irradianceBrief Communications
W. Kupis, E. Tomal, E. Niewiadomska
Biologia Plantarum 63: 405-410, 2019 | DOI: 10.32615/bp.2019.052
Several lines of evidence suggest that plant hormone abscisic acid (ABA) and the activity of alternative respiratory pathway are somehow interrelated. In this paper, we tested how an application of ABA affects the alternative oxidase (AOX) pathway and leaf senescence in Arabidopsis thaliana (L.) Heynh. Different effects of ABA application on the AOX activity were observed under high irradiance (HI) and low irradiance (LI). The expression of AOX1D gene was stimulated, whereas the expression of senescence marker gene WRKY53 was decreased due to ABA treatment. We also noticed that changes of AOX genes AOX1A and AOX1D,...
Somatic mutations, DNA methylation, and expression of DNA repair genes in Arabidopsis thaliana treated with 5-azacytidineOriginal Papers
K.V. Kiselev, Z.V. Ogneva, A.S. Dubrovina, N.N. Nityagovsky, A.R. Suprun
Biologia Plantarum 63: 398-404, 2019 | DOI: 10.32615/bp.2019.051
An inhibitor of DNA methylation 5-azacytidine (5A) is a chemical analog of the nucleoside cytidine. This study investigated the influence of 5A-induced DNA hypomethylation on the accumulation of somatic DNA mutations (nucleotide substitutions, indels) in the Actin2 3′ untranslated region, nuclear internal transcribed spacer ITS1-5.8rRNA-ITS2, and the ribulose-1,5-bisphosphate carboxylase/oxygenase gene of Arabidopsis thaliana and analyzed concurrent changes in the expression of DNA methyltransferase and DNA repair genes. The 5A treatment (20 mg per 100 g of soil) decreased DNA methylation, and the detected 5A-induced demethylation...
N-terminal domains of AhAREB1 protein are necessary for transcriptional characteristics and negative regulation of the AhNCED1 geneOriginal Papers
L. Hong, C.Y. He, H. Shen, S. Liu, X. Liu, X.L. Li, L. Li
Biologia Plantarum 63: 388-397, 2019 | DOI: 10.32615/bp.2019.050
A transcription factor gene Arachis hypogaea abscisic acid (ABA) responsive element binding protein 1 (AhAREB1) has been isolated from peanut previously. Here, the function of different domains from AhAREB1 was investigated using construct series containing AhAREB1 full-length and truncated fragments to transform peanut hairy roots and pAhNCED1 (promoter of Arachis hypogaea 9-cis-epoxycarotenoid dioxygenase 1) GUS/Col Arabidopsis thaliana, respectively. The results of real-time quantitative PCR, transient expression, and chromosome immunoprecipitation (ChIP) assay all showed that AhAREB1 negatively regulated...
EditorialEditorial
J. Pospisilova
Biologia Plantarum 63: i, 2019
Dear members of editorial board, referees, authors, and readers,
First, I greet you at the beginning of the year 2019 and wish you a steady health, a lot of happiness, and a great success in your professional life. Simultaneously, I would like to express many thanks for all the work done and attention paid to our journal in previous years.
Suitable reference genes for real-time quantitative PCR in Salsola laricifilia under five abiotic stressesOriginal Papers
Y.-F. Zhang, Z.-B. Wen, Y. Wang, Y.-L. Wang, Y. Feng
Biologia Plantarum 63: 380-387, 2019 | DOI: 10.32615/bp.2019.044
Salsola laricifolia, a typical C3-C4 intermediate desert plant, is an important for understanding gene evolution and mechanisms for drought resistance. The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a preferred choice for gene expression studies, but it requires stable reference genes for normalization. Therefore, we tested the expression stability of five candidate reference genes in S. laricifolia: EF1α (elongation factor 1-α), ACT (actin), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), TUB (tubulin), and 18S (18S ribosomal RNA). The...
Overexpression of BdRHP1 improves drought tolerance and reduces yield loss in riceOriginal Papers
D.-E. Zeng, Z.-M. Cai, Y.-S. Liu
Biologia Plantarum 63: 371-379, 2019 | DOI: 10.32615/bp.2019.043
Drought is a major factor decreasing the growth, development, and productivity of rice in about one-third of the world area. The characterization of genes imparting tolerance to drought in rice, is an attractive strategy for genetic engineering to improve drought tolerance. It is demonstrated that ectopic overexpression of Brachypodium distachyon RING-H2 finger gene (designated as BdRHP1) enhances drought tolerance in rice at both the vegetative and reproductive stages. When subjected to drought, positive transgenic lines showed delayed wilting, and improved recovery after rewatering. However, the transgenic plants exhibited...
The effects of gibberellic acid on Allium cepa root tip meristematic cellsOriginal Papers
B. Tütünoğlu, Ö. Aksoy, R. Özbek, F. Uçkan
Biologia Plantarum 63: 365-370, 2019 | DOI: 10.32615/bp.2019.042
Gibberellic acid (GA) is a natural plant growth regulator (PGR) which stimulates germination, vegetative growth, flowering, and fruit formation. However, when high concentrations of GA are used, it inhibits plant growth and development and causes abnormalities in the plant tissue. In our study, we determined the effects of different concentrations of GA on Allium cepa L. var. cepa roots. Increasing concentrations of GA (50 - 5 000 mg dm-3) were used in A. cepa root growth inhibition tests. Further, random amplified polymorphic DNA technique was used for determination of possible genotoxic effects of 600 - 1200...
Differential expressions of citrus CAMTAs during fruit development and responses to abiotic stressesOriginal Papers
Z.G. Ouyang, L.F. Mi, H.H. Duan, W. Hu, J.M. Chen, T. Peng, B.L. Zhong
Biologia Plantarum 63: 354-364, 2019 | DOI: 10.32615/bp.2019.041
Calmodulin-binding transcription activators (CAMTAs) play important roles in plant growth, developmental processes, and responses to abiotic and biotic factors. Recently, five CAMTA members were identified in Citrus sinensis, however, very little is known about the molecular regulation of these CAMTAs in citrus during fruit development and under abiotic stresses. In this study, the different expression profiles of CsCAMTA genes were found in different tissues and different fruit developmental stages. The CsCAMTA genes also displayed distinct expression patterns after heat, cold, salt, and drought stresses. Furthermore, the...
Gibberellin A3 mediated decreased transcriptional rate, mRNA stability and non-competitive inhibition of DNA methyltransferases in tobaccoOriginal Papers
R. Manoharlal, G.V.S. Saiprasad, V. Kaikala
Biologia Plantarum 63: 343-353, 2019 | DOI: 10.32615/bp.2019.040
The present work aims to examine the underlying molecular mechanism(s) for the reduced transcriptions and activities of DNA methyltransferases (NtDNMTs) by gibberellin A3 (GA3) in tobacco (Nicotiana tabacum L). Nuclear run-on and cordycepin chase assays demonstrated a significant reduction in the transcriptional rate and transcript half-lives of NtMET1 and NtCMT3 by GA3. Lineweaver-Burk plot analysis revealed a non-competitive mode of inhibition of NtDNMTs activity by GA3. Taken together, both in vivo and in vitro studies clearly demonstrated the altered transcriptional and post-transcriptional regulation...
OsCaM1-1 overexpression in the transgenic rice mitigated salt-induced oxidative damageOriginal Papers
T. Kaewneramit, T. Buaboocha, P. Sangchai, N. Wutipraditkul
Biologia Plantarum 63: 335-342, 2019 | DOI: 10.32615/bp.2019.039
Various physiological and biochemical parameters associated with improved salinity tolerance in the transgenic rice lines overexpressing OsCaM1-1 gene and wild-type KDML105 were compared 3 d after exposure to 150 mM NaCl. The results showed higher relative water content, relative growth rate, content of photosynthetic pigments (chlorophylls a, b, and carotenoids), DPPH scavenging activity, and activities of superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase in the transgenic plants when compared with the wild-type and control, KDML105 transformed with blank vector, whereas H2O2...
Overexpression of the UGT76E12 gene modulates seed germination, growth, and response to NaCl, mannitol, and abscisic acidOriginal Papers
L. Chen, W.-S. Wang, Q. Liu, R.-R. Dong, Y.-J. Li, T.-T Chen, B.-K. Hou
Biologia Plantarum 63: 328-334, 2019 | DOI: 10.32615/bp.2019.038
Seed germination and following seedling growth are largely affected by environmental conditions. However, the genes involved in adaptations to these conditions are largely unknown. In this study, we cloned and characterized an Arabidopsis uridine diphosphate glycosyltransferase gene UGT76E12 and investigated its function in seed germination and plant growth under adverse environments. We found that UGT76E12 gene expression was induced by NaCl, mannitol, and abscisic acid (ABA) treatments. Under these treatments, the UGT76E12 overexpression lines exhibited a delayed seed germination and cotyledon growth compared with...
Cerium nitrate improves salt tolerance of wheat seedlings by regulating the antioxidant capacity of chloroplastsBrief Communications
X.D. Chen, C.J. Shan
Biologia Plantarum 63: 323-327, 2019 | DOI: 10.32615/bp.2019.037
The effects of Ce(NO3)3 on the antioxidant capacity in the chloroplasts of salt-stressed wheat (Triticum aestivum L.) seedlings were investigated. The results showed that salt stress (40, 80, 120, or 160 mM NaCl) significantly increased the activities of ascorbate peroxidase, glutathione reductase, superoxide dismutase, catalase, and glutathione peroxidase, the content of malondialdehyde (MDA) and hydrogen peroxide (H2O2), but significantly reduced the ratios of reduced ascorbate (AsA) to dehydroascorbate (DHA) and reduced glutathione to oxidizided glutathione (GSH/GSSG), the content of chlorophylls...
Biochemical examination of non-transgenic and transgenic soybean plants under drought stress conditionsOriginal Papers
C.G. Gonçalves, A.C. Silva Jr., L.R. Alves, M.R.R. Pereira, P.L. Gratão, D. Martins
Biologia Plantarum 63: 314-322, 2019 | DOI: 10.32615/bp.2019.036
Drought is one of the major environmental stresses limiting crop production worldwide because both the duration and the severity of the stress are critical. Plants display a variety of physiological and biochemical stress responses towards prevailing drought stress thus making it a complex phenomenon. To address the modulation of stress responses, we used Glycine max cv. MG/BR 46 Conquista (non-transgenic) and cv. BRS Valiosa (transgenic) to analyze the effects of progressive drought stress during two stages of soybean development (V2 and V4) on physiological and biochemical parameters. Three minimum soil water potentials (Ψs) were established:...
The RNA-seq transcriptome analysis identified genes related to rice seed dormancyOriginal Papers
K. Xie, J. Bai, Y.Y. Yang, N.B. Duan, Y.M. Ma, T. Guo, F.Y. Yao, H.F. Ding
Biologia Plantarum 63: 308-313, 2019 | DOI: 10.32615/bp.2019.035
Plant hormones play important roles in seed dormancy and dormancy breaking. We measured the hormone content in rice (Oryza sativa L. cv. Nona Bokra) seeds at different stages and with or without imbibition treatment. We identified 1 265 differentially expressed genes (DEGs) between dormant and dormancy-broken seeds using RNA-seq analysis: 1 015 genes were significantly up-regulated, while 250 genes were significantly down-regulated. Sixteen DEGs were selected as related to seed dormancy, and their expressions were validated using quantitative PCR. Three DEGs were in the same position as two reported dormancy QTLs, suggesting that they...
Effect of aluminum and fluoride on R2R3-MYB transcription factor characterization and expression in Camellia sinensisOriginal Papers
Y. Wang, P. Chang, J. Pan, J. Zhu, C. Cui, X. Ye, Y. Ma, X. Zhu, W. Fang, C. Jiang
Biologia Plantarum 63: 298-307, 2019 | DOI: 10.32615/bp.2019.034
Tea plant (Camellia sinensis) is a typical plant that accumulates abundant aluminum (Al) and fluoride (F). Al and F play vital roles in the growth and development in tea plant. MYB (myeloblastosis) is one of the largest transcription factor families in plant, which plays an important role in the stress responses. As the largest and multifunctional subfamily of MYB, R2R3-MYB subfamily participates in multiple abiotic stresses in plant. However, there are few studies about R2R3-MYB in tea plant, especially in the accumulation of Al and F during its growth. Here, we identified 43 CsMYB genes from the transcriptome database of tea plant...
Chl a fluorescence and proteomics reveal protection of the photosynthetic apparatus to dehydration in tolerant but not in susceptible wheat cultivarsOriginal Papers
M. Nykiel, P. Lisik, J. Dębski, B. Florea, K. Rybka
Biologia Plantarum 63: 287-297, 2019 | DOI: 10.32615/bp.2019.033
Seedlings of spring wheat (Triticum aestivum L.) cultivars, Ethos and Zebra, differing in drought tolerance were dehydrated to reach a water saturation deficit (WSD) in leaves ~15, 30, and 50 %. Ethos, the drought tolerant cultivar, dried slower in comparison with Zebra and regrew in 70 % upon rehydration. The effect of dehydration on photosystem II was evaluated by Chl a fluorescence (OJIP transients). The inflection point of double normalized curves (ΔWOJ) calculated for Ethos was negative for seedlings with 15 % WSD, nearly zero for those with 30 % WSD, and about +0.05 for those with 50 % WSD. In case of Zebra, the...
Shoot proliferation and organogenesis on Arbutus unedo: physiological analysis under water stressOriginal Papers
J.F. Martins, S. Correia, B. Correia, G. Pinto, J.M. Canhoto
Biologia Plantarum 63: 278-286, 2019 | DOI: 10.32615/bp.2019.032
Strawberry tree (Arbutus unedo) is a small perennial tree that grows spontaneously in the Mediterranean basin, Ireland, and Portugal. In this work, strawberry tree clones were established in vitro from epicormic shoots obtained from a young tree, an adult tree, and from a seedling. They were propagated by axillary shoot buds proliferation on solid and in liquid media, and also in a modified De Fossard medium with 9 µM benzylaminopurine. The organogenesis from calli obtained from apical leaves of the in vitro grown shoots from the three genotypes was carried out in the same basal liquid medium supplemented with 9 µM...
Heterologous expression of the AtDREB1A gene in tomato confers tolerance to chilling stressOriginal Papers
S.G. Karkute, R. Krishna, W.A. Ansari, B. Singh, P.M. Singh, M. Singh, A.K. Singh
Biologia Plantarum 63: 268-277, 2019 | DOI: 10.32615/bp.2019.031
Tomato is highly sensitive to chilling stress (0 - 12 °C) which severely affects plant growth and development. Transgenic tomato plants expressing the AtDREB1A gene under the control of the rd29A promoter were evaluated for its tolerance to chilling stress by exposing them to 4 °C for 5 d. The cold stress caused an increase in production of reactive oxygen species, however, transgenic plants had an effective antioxidant system due to an enhanced synthesis of catalase (CAT), superoxide dismutase (SOD), and ascorbate and so the reduced content of hydrogen peroxide and superoxide anions. Transgenic plants showed a slightly less reduction of chlorophyll...