Organic Chemistry

Tutor	Prof. Ing. Michal Hocek, PhD. DSc.
Team	Michal Hocek
Topic	New biorthogonal reactions on DNA
Faculty	FCHT or FPBT VŠCHT, PřF UK
Abstract	New bioorthogonal reactions (chemical or enzymatic) in major or minor groove of DNA will be developer and studied for applications in DNA labelling, bioconjugations, and detection and imaging of epigenetic bases or DNA lesions. Leading references: 1. Dadová, J.; Orság, P.; Pohl, R.; Brázdová, M.; Fojta, M.; Hocek, M. "Vinylsulfonamide and acrylamide modification of DNA for cross-linking with proteins" Angew. Chem. Int. Ed. 2013, 52, 10515-10518. 2. Olszewska, A.; Pohl, R.; Brázdová, M.; Fojta, M.; Hocek, M. "Chloroacetamide-linked nucleotides and DNA for cross-linking with peptides and proteins" Bioconjug. Chem. 2016, 27, 2089–2094. 3. Slavíčková, M.; Janoušková, M.; Šimonová, A.; Cahová, H.; Kambová, M.; Šanderová, H.; Krásný, L.; Hocek, M. "Turning off transcription with bacterial RNA polymerase through CuAAC click reactions of DNA containing 5-ethynyluracil" Chem. Eur. J. 2018, 24, 8311-8314.