Biologia plantarum 56:25-30, 2012 | DOI: 10.1007/s10535-012-0011-8

Adventitious rooting of detached Arabidopsis thaliana leaves

L. R. Correa1, R. J. Stein2, A. G. Fett-Neto1,2,*
1 Departamento de Botânica, Laboratório de Fisiologia Vegetal, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
2 Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

Arabidopsis thaliana Heyhn is a model species in biochemical, physiological and molecular studies for which a plethora of mutants is available. This work aimed at developing a system for rooting of detached leaves, and evaluating time course of several relevant biochemical parameters during rooting assays with and without auxins. The rooting pattern was of the direct type (without callus formation) in all of the treatments and ecotypes analyzed and was rather stable. Considering the different parameters examined, peroxidase activity and contents of phenolic compounds and soluble sugars appeared as the most distinct biochemical markers of the rooting process in this system.

Keywords: auxins; peroxidases; phenolics; root development; sugars
Subjects: auxins; phenolics; root development; sugars; adventitious rooting; indoleacetic acid; indolebutyric acid; naphthaleneacetic acid; peroxidase; chlorophyll; anthocyanins; flavonoids; starch; indoleacetic acid oxidase
Species: Arabidopsis thaliana

Received: October 9, 2009; Accepted: January 17, 2011; Published: March 1, 2012Show citation

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Correa, L.R., Stein, R.J., & Fett-Neto, A.G. (2012). Adventitious rooting of detached Arabidopsis thaliana leaves. Biologia plantarum, 56(1), 25-30. doi: 10.1007/s10535-012-0011-8.
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